Characterization of a bovine herpesvirus type 4 isolated from the spinal cord of a cow with astasia

Abstract

A bovine herpesvirus (BHV) strain designated B11-41, was recently isolated from the spinal cord of a cow with astasia. By BHV type specific polymerase chain reaction (PCR), a BHV-4 specific fragment was amplified from the DNA of strain B11-41. The nucleotide sequence of the PCR product revealed high homologies (98.4% and 99.8%) with those of two BHV-4 strains (Movar 33/63 and 86-068). The BamHI restriction endonuclease cleavage patterns of the B11-41 were more similar to those of BHV-4 than those of other types of BHV. BHV-4 is divided into two groups, European and American. The EcoRI, and BamHI, and HindIII restriction endonuclease analysis demonstrated that strain B11-41 was closely related to the American type of BHV-4. It was concluded that B11-41 was identified as a BHV-4 that belongs to the American group. Additionally, the results of this study may indicate that the nervous system is one of the sites of viral latency in natural infection.

Latency and Persistence of Bovine Herpesvirus Type 4, Strain B11-41, in Bovine Nervous Tissues.

“…Viruses: Strain B11-41 of BoHV-4, isolated from the spinal cord of a cow with astasia, was used in this study [21,30]. The Los Angeles strain of bovine herpesvirus type 1 (BoHV-1) [18], preserved in the National Institute of Animal Health, Tsukuba, Japan, was used as a control to determine the specificity of the enzyme-linked immunosorbent assay (ELISA).…”

“…However, since gammaherpesviruses usually persist latently in lymphocytes, and since BoHV-4 has been isolated from a wide variety of clinical conditions in cattle, it is not evident that persistent infection in the nervous system is a common biological property of all BoHV-4 strains. Recently strain B11-41 of BoHV-4, which belongs to the DN 599-like group, was isolated from the spinal cord of a cow with astasia in Japan [21,30]. Our aims in the present study were to investigate the pathogenicity and tissue tropism of the virus in cattle.…”

Characterization of Bovine Herpesvirus Type 4 Isolated from Cattle with Mastitis and Subclinical Infection by the Virus among Cattle

“…Movar-like viruses have mainly been isolated in Europe, and DN 599-like viruses have mainly isolated in North America. In Japan, strain B11-41 of BoHV-4, which belongs to the DN 599-like group, was first isolated from the spinal cord of a cow with astasia [24]. Recently, a BoHV-4 strain was isolated from a mammary lesion of a cow with clinical mastitis from a farm in Fukushima prefecture in Japan [17].…”

“…DNA was extracted from the cell pellet using a commercially available test kit (SepaGene, Sanko Junyaku Co., Ltd., Tokyo, Japan) following the manufacturer's instructions, dissolved in a small volume of TE buffer (10 mM Tris-HCl, pH 8.0 and 1 mM EDTA) and then the DNA concentration was measured. Strain B11-41 [24], Movar 33/63 and 86-068 [2,18] were used as controls for BoHV-4. BoHV-1 (strain 758) [19] and BoHV-2 (Minnesota strain) [25] were also used as controls for bovine herpesviruses.…”

Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2.

“…Other animal DNA viruses used in this study are: a Los Angeles strain of bovine herpesvirus 1 (BoHV-1) [11], a WC-11 strain of alcelaphine herpesvirus 1 (AlHV-1) [12], ovine herpesvirus 2 (OvHV-2) [13], an HH-1 strain of equine herpesvirus 1 (EHV-1) [8], a YS-81 strain of pseudorabies virus (PRV) [2], and a Fukuroi strain of bovine adenovirus 7 (BAdV-7) [7]. Additionally, DNA samples extracted from a Chiba strain of parapoxvirus (PPV) [9] and a B11-41 strain of bovine herpesvirus 4 (BoHV-4) [15] were provided by Dr. H. Sentsui, National Institute of Animal Health, Japan.…”