Characterization of Bovine Herpesvirus Type 4 Isolated from Cattle with Mastitis and Subclinical Infection by the Virus among Cattle

Izumi, Yasuhiro; Tsuduku, Satoko; Murakami, Kenji; Tsuboi, T.; Konishi, Misako; Haritani, Makoto; Kamiyoshi, Takeshi; Kimura, Kumiko; Sentsui, Hiroshi

doi:10.1292/jvms.68.189

Cited by 23 publications

(25 citation statements)

References 21 publications

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“…Our results suggest that BoHV-4 can potentially have a role in bovine diseases, i.e., can affect the respiratory and reproductive systems of animals. The possibility that BoHV-4 may play a causal role in the development of disease is still uncertain, because the virus has been found in both clinical cases (4, 18, 21, 22, 25, 28) and healthy asymptomatic animals. Nevertheless, an increasing number of studies consider BoHV-4 as a risk factor for reproductive tract infections (21, 22, 31).…”

Section: Discussionmentioning

confidence: 99%

Whole Genome Sequencing of a Canadian Bovine Gammaherpesvirus 4 Strain and the Possible Link between the Viral Infection and Respiratory and Reproductive Clinical Manifestations in Dairy Cattle

et al. 2017

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Bovine gammaherpesvirus 4 (BoHV-4) is a herpesvirus widespread in cattle populations, and with no clear disease association. Its genome contains a long unique coding region (LUR) flanked by polyrepetitive DNA and 79 open reading frames (ORFs), with unique 17 ORFs, named Bo1 to Bo17. In 2009, a BoHV-4 strain was isolated (FMV09-1180503: BoHV-4-FMV) from cattle with respiratory disease from Quebec, Canada, and its LUR was sequenced. Despite the overall high similarity, BoHV-4-FMV had the most divergent LUR sequence compared to the two known BoHV-4 reference strain genomes; most of the divergences were in the Bo genes and in the repeat regions. Our phylogenetic analysis based on DNA polymerase and thymidine kinase genes revealed that virus isolate was BoHV-4 gammaherpesvirus and clustered it together with European BoHV-4 strains. Because BoHV-4-FMV was isolated from animals presenting respiratory signs, we have updated the BoHV-4 Canadian cattle seroprevalence data and tried to find out whether there is a link between clinical manifestation and BoHV-4 seropositivity. An indirect immunofluorescence assay (IFA) was performed with nearly 200 randomized sera of dairy cattle from two Canadian provinces, Quebec (n = 100) and Ontario (n = 91). An additional set of sera obtained from Quebec, from the healthy (n = 48) cows or from the animals experiencing respiratory or reproductive problems (n = 75), was also analyzed by IFA. BoHV-4 seroprevalence in Canadian dairy cattle was 7.9% (Quebec: 6% and Ontario: 9.9%). Among animals from the Quebec-based farms, diseased animals showed higher BoHV-4 seropositivity than healthy animals (P < 0.05), with a significant 2.494 odds ratio of being seropositive in sick compared to healthy animals. Although there is no established direct link between BoHV-4 and specific diseases, these seroprevalence data suggest the possible involvement of BoHV-4 in dairy cattle diseases.

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Section: Discussionmentioning

confidence: 99%

Whole Genome Sequencing of a Canadian Bovine Gammaherpesvirus 4 Strain and the Possible Link between the Viral Infection and Respiratory and Reproductive Clinical Manifestations in Dairy Cattle

et al. 2017

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“…La transmisión horizontal ocurre por contacto directo e indirecto mediante secreciones y fómites. El principal modo de transmisión del BoHV-4 aún no ha sido establecido con certeza; hay evidencias experimentales de excreción viral intermitente y detección de ADN viral en leche 23,51,90 . Aunque los datos sobre aislamiento y detección de virus en semen son escasos 17,41,72,76 , se comprobó la transmisión de cepas endometriotrópicas europeas por esta vía 47 .…”

Section: Epidemiologíaunclassified

“…El BoHV-4 ha sido aislado tanto de bovinos sanos como de ejemplares con una amplia variedad de signos clínicos (conjuntivitis, enfermedad respiratoria, vaginitis, metritis, linfosarcoma, dermatitis, endometritis supurativa posparto, mastitis) en diferentes partes del mundo 7,21,51,69,75 . También se ha detectado alto nivel de infección por BoHV-4 en bovinos lecheros con repetición de celo 53 .…”

Section: Epidemiologíaunclassified

“…El BoHV-4 ha sido aislado en una amplia variedad de casos clínicos, así como de animales aparentemente sanos, en diferentes partes del mundo 6,7,23,51,69,75,88 . Si bien no se ha reconocido como agente causal de una entidad patológica específica, se asocia mayormente con infecciones del tracto reproductivo de los bovinos, particularmente durante el período posparto 17 .…”

Section: Introductionunclassified

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Herpesvirus bovino 4 (BoHV-4): aspectos generales de su biología y situación en la República Argentina

Morán

Pérez

Odeón

et al. 2015

Revista Argentina de Microbiología

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Bovine herpesvirus 4 (BoHV-4) has been isolated from cattle with respiratory infections, vulvovaginitis, mastitis, abortions, endometritis and from apparently healthy animals throughout the world. Although it has not yet been established as causal agent of a specific disease entity, it is primarily associated with reproductive disorders of cattle. This virus can infect a wide range of species, either in vivo or in vitro. Two groups of prototype strains were originated from the first isolates: the DN599-type strains (American group) and the Movar-type strains (European group). In Argentina, BoHV-4 was isolated and characterized in 2007 from vaginal discharge samples taken from cows that had aborted. So far, more than 40 isolates, mainly associated with aborting bovine females have been registered in our country.

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“…BoHV-4 has been isolated from cattle with respiratory infections, vulvovaginitis, mastitis, abortions, endometritis and apparently healthy animals throughout the world (Wellemans et al 1986, Miyano et al 2004, Izumi et al 2006, Bilge-Dagalp et al 2010, 2012. lions, domestic cats and rabbits (Kruger et al 2000, Lin et al 2000, Kálmán & Egyed 2005, Dewals et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves

Morán

Pérez

Garcia

et al. 2017

VR&R

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Although bovine herpesvirus type 4 (BoHV-4) is primarily associated with reproductive disorders of cattle, it can produce a variety of clinical signs. To determine the distribution, the presence and type of microscopic lesions caused by BoHV-4 strains of different genotypes an in vivo model, calves were infected with three phylogenetically different Argentinean BoHV-4 strains. Samples from nasal and ocular secretions, peripheral blood leukocytes, tissues and serum were analyzed. BoHV-4 was isolated from nasal and ocular secretions at 7 and 14 days post-inoculation (dpi). Viral DNA was detected by nested PCR in peripheral blood leukocytes at 14 and 21 dpi for two out of three strains and in tissues, such as nervous system, trachea, pulmonary and retropharyngeal lymph nodes, spleen and kidney, at 21 dpi. Antibody levels detected by viral seroneutralization test were mostly low and varied widely for the different strains. The tissue distribution of the BoHV-4 strains and the variations observed in the levels of neutralizing antibodies indicate that certain differences can be established among the patterns of biological behavior of each strain. This is an initial step to get insight into the biological characteristics of Argentinean BoHV-4 isolates. However, further evaluation involving a higher number of inoculated animals will be required to be conclusive on this aspect.

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Characterization of Bovine Herpesvirus Type 4 Isolated from Cattle with Mastitis and Subclinical Infection by the Virus among Cattle

Cited by 23 publications

References 21 publications

Whole Genome Sequencing of a Canadian Bovine Gammaherpesvirus 4 Strain and the Possible Link between the Viral Infection and Respiratory and Reproductive Clinical Manifestations in Dairy Cattle

Whole Genome Sequencing of a Canadian Bovine Gammaherpesvirus 4 Strain and the Possible Link between the Viral Infection and Respiratory and Reproductive Clinical Manifestations in Dairy Cattle

Herpesvirus bovino 4 (BoHV-4): aspectos generales de su biología y situación en la República Argentina

Tissular Distribution of Argentinean Strains of Bovine Herpesvirus Type 4 (BoHV-4) in Experimentally-Infected Calves

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