Characterization, expression, enzymatic activity, and functional identification of cathepsin S from black rockfish Sebastes schlegelii

Wang, Guanghua; He, Shuwen; Du, Xiang; Xie, Bing; Gu, Qin-qin; Zhang, Min; Hu, Yonghua

doi:10.1016/j.fsi.2019.08.012

Cited by 12 publications

(1 citation statement)

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“…As cathepsins play vital roles in immune responses to bacterial infections, clarification of the molecular mechanism for cathepsins in response to A. salmonicida infection is expected to promote the development of effective strategies in bacterial disease management for S. schlegelii . Previous studies reported three members of the cathepsin family (CTSB, CTSK, and CTSSb) in S. schlegelii [ 23 , 36 , 37 ]. In this study, we first systematically identified all possible members of the cathepsin superfamily in S. schlegelii .…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Evolutionary Analysis, and Expression Patterns of Cathepsin Superfamily in Black Rockfish (Sebastes schlegelii) following Aeromonas salmonicida Infection

Zhang

Chen

et al. 2022

Marine Drugs

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Cathepsins are lysosomal cysteine proteases belonging to the papain family and play crucial roles in intracellular protein degradation/turnover, hormone maturation, antigen processing, and immune responses. In the present study, 18 cathepsins were systematically identified from the fish S. schlegelii genome. Phylogenetic analysis indicated that cathepsin superfamilies are categorized into eleven major clusters. Synteny and genome organization analysis revealed that whole-genome duplication led to the expansion of S. schlegelii cathepsins. Evolutionary rate analyses indicated that the lowest Ka/Ks ratios were observed in CTSBa (0.13) and CTSBb (0.14), and the highest Ka/Ks ratios were observed in CTSZa (1.97) and CTSZb (1.75). In addition, cathepsins were ubiquitously expressed in all examined tissues, with high expression levels observed in the gill, intestine, head kidney, and spleen. Additionally, most cathepsins were differentially expressed in the head kidney, gill, spleen, and liver following Aeromonas salmonicida infection, and their expression signatures showed tissue-specific and time-dependent patterns. Finally, protein–protein interaction network (PPI) analyses revealed that cathepsins are closely related to a few immune-related genes, such as interleukins, chemokines, and TLR genes. These results are expected to be valuable for comparative immunological studies and provide insights for further functional characterization of cathepsins in fish species.

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Section: Introductionmentioning

confidence: 99%