Identification and characterization of a cathepsin K homologue that interacts with pathogen bacteria in black rockfish, Sebastes schlegelii

He, Shuwen; Du, Xiang; Wang, Guanghua; Wang, Jingjing; Xie, Bing; Gu, Qin-qin; Zhang, Min; Gu, Hanjie

doi:10.1016/j.fsi.2020.01.050

Cited by 10 publications

(7 citation statements)

References 42 publications

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“…In addition to normal physiological functions [ 26 , 27 , 28 ], Cathepsin K also exhibits deleterious effects on the body, as evidenced by its role in the progression of a variety of tumors. Over the past few years, accumulated data have shown overexpression of Cathepsin K in multiple cancer types, indicating its role in tumor progression and its potential diagnostic and prognostic values.…”

Section: Discussionmentioning

confidence: 99%

The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

et al. 2020

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(1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lung fibroblast MRC-5 cells using Western blot and immunofluorescence assay. Cathepsin K was transiently overexpressed or knocked down using transfection with a recombinant plasmid and siRNA, respectively, to test the effects on cell proliferation, migration, invasion, and on the mammalian target of rapamycin (mTOR) signaling pathway. (3) Results: Expression of Cathepsin K was increased significantly in A549 cells and diffused within the cytoplasm compared to the MRC-5 cells used as control. Cathepsin K overexpression promoted the proliferation, migration, and invasion of A549 cells, accompanied by mTOR activation. Cathepsin K knockdown reversed the above malignant behavior and inhibited the mTOR signaling activation, suggesting that Cathepsin K may promote the progression of NSCLC by activating the mTOR signaling pathway. (4) Conclusion: Cathepsin K may potentially represent a viable drug target for NSCLC treatment.

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Section: Discussionmentioning

confidence: 99%

The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

et al. 2020

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“…GO analysis showed that these target genes were mainly enriched in the 3,245 GO term process (Figure 3E). Analysis of the KEGG metabolic pathway showed that these genes were involved in metabolic pathways (158), MAPK signaling pathway (47), calcium signaling pathway (35), protein processing in the endoplasmic reticulum (28), focal adhesion (28), tight junction (24), and apoptosis (25) (Figure 3F).…”

Section: Identification Of De Mirnas Between E Tarda-infected and Control Groupsmentioning

confidence: 99%

“…Therefore, studies on black rockfish immune-related genes can expand our understanding of ncRNA that related to immune response and regulatory mechanisms, and are also helpful to guide the prevention and control of its diseases. Previous studies have investigated several immune-related genes, such as high mobility group box 1, c-type lectin, galectin-8, cathepsin K, chemokine ligand 25, and melanocortin-4, in black rockfish in response to pathogen stimulation (32)(33)(34)(35)(36)(37). However, no systematic report on the interactions of ncRNAs and coding RNAs in S. schlegelii during infection has been performed.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

Cao

et al. 2021

Front. Immunol.

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Sebastes schlegelii, an important aquaculture species, has been widely cultured in East Asian countries. With the increase in the cultivation scale, various diseases have become major threats to the industry. Evidence has shown that non-coding RNAs (ncRNAs) have remarkable functions in the interactions between pathogens and their hosts. However, little is known about the mechanisms of circular RNAs (circRNAs) and coding RNAs in the process of preventing pathogen infection in the intestine in teleosts. In this study, we aimed to uncover the global landscape of mRNAs, circRNAs, and microRNAs (miRNAs) in response to Edwardsiella tarda infection at different time points (0, 2, 6, 12, and 24 h) and to construct regulatory networks for exploring the immune regulatory mechanism in the intestine of S. schlegelii. In total, 1,794 mRNAs, 87 circRNAs, and 79 miRNAs were differentially expressed. The differentially expressed RNAs were quantitatively validated using qRT-PCR. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that most of the differentially expressed mRNA genes and the target genes of ncRNAs were related to immune signaling pathways, such as the NF-κB signal pathway, pathogen recognition receptors related to signaling pathways (Toll-like receptors and Nod-like receptors), and the chemokine signaling pathway. Based on these differentially expressed genes, 624 circRNA-miRNA pairs and 2,694 miRNA-mRNA pairs were predicted using the miRanda software. Integrated analyses generated 25 circRNA-miRNA-mRNA interaction networks. In a novel_circ_0004195/novel-530/IκB interaction network, novel_530 was upregulated, while its two targets, novel_circ_0004195 and IκB, were downregulated after E. tarda infection. In addition, two circRNA-miRNA-mRNA networks related to apoptosis (novel_circ_0003210/novel_152/apoptosis-stimulating of p53 protein 1) and interleukin (novel_circ_0001907/novel_127/interleukin-1 receptor type 2) were also identified in our study. We thus speculated that the downstream NF-κB signaling pathway, p53 signaling pathway, and apoptosis pathway might play vital roles in the immune response in the intestine of S. schlegelii. This study revealed a landscape of RNAs in the intestine of S. schlegelii during E. tarda infection and provided clues for further study on the immune mechanisms and signaling networks based on the circRNA-miRNA-mRNA axis in S. schlegelii.

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“…Cathepsin H plays important roles in the intracellular degradation of proteins and antigen processing [ 21 ] and is also required for disease treatment and lung development [ 22 ]. Cathepsin K stands out for several reasons, such as its central role in bone resorption, bone erosion, and immune regulation [ 23 ]. Cathepsin L has been revealed to play vital roles in controlling normal mucosal epithelial homeostasis and supporting the host immune defense against infection in mammal mucosal surfaces [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification, Evolutionary Analysis, and Expression Patterns of Cathepsin Superfamily in Black Rockfish (Sebastes schlegelii) following Aeromonas salmonicida Infection

Zhang

Chen

et al. 2022

Marine Drugs

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Cathepsins are lysosomal cysteine proteases belonging to the papain family and play crucial roles in intracellular protein degradation/turnover, hormone maturation, antigen processing, and immune responses. In the present study, 18 cathepsins were systematically identified from the fish S. schlegelii genome. Phylogenetic analysis indicated that cathepsin superfamilies are categorized into eleven major clusters. Synteny and genome organization analysis revealed that whole-genome duplication led to the expansion of S. schlegelii cathepsins. Evolutionary rate analyses indicated that the lowest Ka/Ks ratios were observed in CTSBa (0.13) and CTSBb (0.14), and the highest Ka/Ks ratios were observed in CTSZa (1.97) and CTSZb (1.75). In addition, cathepsins were ubiquitously expressed in all examined tissues, with high expression levels observed in the gill, intestine, head kidney, and spleen. Additionally, most cathepsins were differentially expressed in the head kidney, gill, spleen, and liver following Aeromonas salmonicida infection, and their expression signatures showed tissue-specific and time-dependent patterns. Finally, protein–protein interaction network (PPI) analyses revealed that cathepsins are closely related to a few immune-related genes, such as interleukins, chemokines, and TLR genes. These results are expected to be valuable for comparative immunological studies and provide insights for further functional characterization of cathepsins in fish species.

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Identification and characterization of a cathepsin K homologue that interacts with pathogen bacteria in black rockfish, Sebastes schlegelii

Cited by 10 publications

References 42 publications

The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

Integrated Analysis of circRNA-miRNA-mRNA Regulatory Networks in the Intestine of Sebastes schlegelii Following Edwardsiella tarda Challenge

Genome-Wide Identification, Evolutionary Analysis, and Expression Patterns of Cathepsin Superfamily in Black Rockfish (Sebastes schlegelii) following Aeromonas salmonicida Infection

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