Characterization and refinement of zein-based gels

Gagliardi, Agnese; Froiio, Francesca; Salvatici, Maria Cristina; Paolino, Donatella; Fresta, Massimo; Cosco, Donato

doi:10.1016/j.foodhyd.2019.105555

Cited by 55 publications

(31 citation statements)

References 38 publications

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“…The stability of the GMO-nanostructures was evaluated using a Turbiscan Lab ® Expert (Formulaction, Toulouse, France) apparatus following the variation of their backscattering (ΔBS) profiles as a function of time. The data were processed using a Turby Soft 2.0 (Formulaction, Toulouse, France) and expressed as Turbiscan stability index (TSI) versus time [ 27 , 28 ]. The TSI values were calculated using Equation (1) with a specific computer program:

where x i is the backscattering for each minute of measurement, x BS is the mean x i , and n is the number of scans [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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Glyceryl monooleate (GMO) is one of the most popular amphiphilic lipids, which, in the presence of different amounts of water and a proper amount of stabilizer, can promote the development of well defined, thermodynamically stable nanostructures, called lyotropic liquid crystal dispersions. The aim of this study is based on the design, characterization, and evaluation of the cytotoxicity of lyotropic liquid crystal nanostructures containing a model anticancer drug such as doxorubicin hydrochloride. The drug is efficiently retained by the GMO nanosystems by a remote loading approach. The nanostructures prepared with different non-ionic surfactants (poloxamers and polysorbates) are characterized by different physico-chemical features as a function of several parameters, i.e., serum stability, temperature, and different pH values, as well as the amount of cryoprotectants used to obtain suitable freeze-dried systems. The nanostructures prepared with poloxamer 407 used as a stabilizer show an increased toxicity of the entrapped drug on breast cancer cell lines (MCF-7 and MDA-MB-231) due to their ability to sensitize multidrug-resistant (MDR) tumor cells through the inhibition of specific drug efflux transporters. Moreover, the interaction between the nanostructures and the cells occurs after just a few hours, evidencing a huge cellular uptake of the nanosystems.

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where x i is the backscattering for each minute of measurement, x BS is the mean x i , and n is the number of scans [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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“…Several studies have described the rheological behavior of specific fractions of zein such as α- and γ-zein dispersions, obtained after the purification of the protein [ 17 , 18 , 19 ], and different materials such as glycerol, β-carotene, acrylic acid, propylene glycol alginate, tannic acid, pea protein, citric acid and acetic anhydride were added to modulate the gelling features of zein [ 20 , 21 , 22 , 23 , 24 , 25 ]. However, our research team recently used commercial zein, at a considerably lower cost than that of the purified form, to prepare gels (up to 20% w / v of protein) without any chemical refinement or the addition of gelling agents; for the first time, the characterization of zein gels was performed under static and dynamic conditions [ 26 ]. During the phases of characterization of a formulation, the effects of the compound(s) to be entrapped on the characteristics of the system need to be evaluated; for this reason, different molecules were added to the zein gels in order to investigate their impact on the features of these protein systems.…”

Section: Introductionmentioning

confidence: 99%

Influence of Various Model Compounds on the Rheological Properties of Zein-Based Gels

et al. 2020

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The controlled release of a compound entrapped in a biocompatible formulation is a sought-after goal in modern pharmaceutical technology. Zein is a hydrophobic protein which has several advantageous properties that make it suitable for use as a biocompatible and degradable material under physiological conditions. It is, therefore, proposed for different biomedical and pharmaceutical applications. In particular, due to its gelling properties, it can be used to form a polymeric network able to preserve biomolecules from harsh environments. The current study was designed to investigate the influence of different probes on the rheological properties of gels made up of zein, in order to characterize the systems as a function of the polymer concentration. Four model compounds characterized by different physico-chemical properties were entrapped in zein gels, and different behaviors (viscoelastic or pronounced solid-like characteristics) of the systems were observed. Zein-based gels showed various release profiles of the encapsulated compounds, suggesting that there are different interaction rates between the probes and the polymeric matrix.

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“…Zein-based materials have been used as vehicles in pharmaceutical formulations [20,52]. For instance, zein microspheres [53] and bionanocomposites [54] have been used for drug delivery.…”

Section: Biomedical Applications: Drug Releasementioning

confidence: 99%

“…In the present study, we explore the use of zein, the most abundant constitutive protein in maize seeds, as a suitable material for developing 3D printing materials. Zein, as a byproduct of corn syrup or cornstarch production, is a widely abundant and cost-effective plant-derived biopolymer [19,20]. It is a prolamine, a protein rich in the amino acid proline, and occurs as aggregates linked by disulfide bonds [21,22].…”

Section: Introductionmentioning

confidence: 99%

“…Zein is biodegradable, edible, and has been classified as "generally recognized as safe" (GRASS) by the FDA [24]. It has been used in biomedical applications, such as the coating for pharmaceutical capsules [19,20,23] or the fabrication of scaffolds for cell culture [25]. To our knowledge, zein has never been explored as a 3D printing material, even though concentrated solutions exhibit shear thinning properties [26], which are highly favorable for extrusion-based 3D printing.…”

Section: Introductionmentioning

confidence: 99%

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Biofabrication using maize protein: 3D printing using zein formulations

Negrete

Aceves-Colin

Rivera-Flores

et al. 2020

Preprint

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The use of three-dimensional (3D) printing for biomedical applications has expanded exponentially in recent years. However, the current portfolio of 3D printable inks is still limited. For instance, only a few protein matrices have been explored as printing/bioprinting materials. Here, we introduce the use of zein, the primary constitutive protein in maize seeds, as a 3D-printable material. Zein-based inks were prepared by dissolving commercial zein powder in ethanol with or without polyethylene glycol (PEG400) as a plasticizer. The rheological characteristics of our materials, studied during 21 days of aging/maturation, showed an increase in the apparent viscosity as a function of time in all formulations. The addition of PEG 400 decreased the apparent viscosity. Inks with and without PEG400 and at different maturation times were tested for printability in a BioX bioprinter. We optimized the 3D printing parameters for each ink formulation in terms of extrusion pressure and linear printing velocity. Higher fidelity structures were obtained with inks that had maturation times of 10 to 14 days. We present different proof-of-concept experiments to demonstrate the versatility of the engineered zein inks for diverse biomedical applications. These include printing of complex and/or free-standing 3D structures, materials for controlled drug release, and scaffolds for cell culture.

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Characterization and refinement of zein-based gels

Cited by 55 publications

References 38 publications

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Influence of Various Model Compounds on the Rheological Properties of Zein-Based Gels

Biofabrication using maize protein: 3D printing using zein formulations

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