Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent

Tonello, Giuseppe; Daglio, M.; Zaccarelli, Nadia; Sottofattori, Enzo; Mazzei, Mauro; Balbi, Alessandro

doi:10.1016/0731-7085(96)01788-8

Cited by 88 publications

(69 citation statements)

References 9 publications

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“…Such PEs reportedly contained enzymes such as alkaline acid phosphatase and glutamic oxalo acetic transaminase, as well as nucleic acids, vitamins, amino acids, steroids, fatty acids, and minerals [2]. PEs are a traditional medicine used for wound healing, because of immune tropic and anti-inflammatory activities, and as a cosmetic supplement in many Asian countries [3]. In Asia, subcutaneous or intramuscular injection of Human PE (HPE), a hydrolysate of the human placenta commercially named Laennec (Japan Bio Products, Tokyo, Japan), has been recently used for the treatment of various disorders including climacteric symptoms, non-healing wounds and chronic varicose ulcers [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Yoshikawa

Takano²,

Ishigaki³

et al. 2013

Gynecol Obstet (Sunnyvale)

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Placental Extract (PE) has been used as a folk remedy in Asian countries. Human PE is clinically used for the treatment of skin wrinkles because it promotes skin regeneration. In this study, we found that Porcine PE (PPE) promoted collagen production in Normal Human Dermal Fibroblasts (NHDFs). Treatment of NHDFs with PPE at concentrations of 12.5-100 μg/mL enhanced their proliferation and secretion of procollagen C peptide. We also found that expression of intracellular Matrix Metalloproteinase-9 (MMP-9) in PPE-treated NHDFs was decreased in a dose-dependent manner. These results indicate that PPE might affect facial wrinkling partly by enhancing fibroblast proliferation and collagen secretion while inhibiting MMP-9 expression in NHDFs.

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Section: Introductionmentioning

confidence: 99%

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Yoshikawa

Takano²,

Ishigaki³

et al. 2013

Gynecol Obstet (Sunnyvale)

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“…Polydeoxyribonucleotide (PDRN), an active component composed of different lengths of deoxyribonucleotide polymers extracted from placenta activates the salvage pathway for the biosynthesis of nucleosides, nucleotides and nucleic acids [29]. In addition, PDRN were shown to exhibit wound healing function especially for the treatment of ulcers, wounds and scars [28,30].…”

Section: Nucleic Acidsmentioning

confidence: 99%

Placental therapy: An insight to their biological and therapeutic properties

Pan¹,

Chan²,

Wong³

et al. 2017

J Med Therap

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The placenta, supporting the growth and development of the fetus, are rich in amino acids, peptides, vitamins, trace elements and growth factors, nutrients and biological active components. Placental therapy was applied to promote from the recovery of diseases and tissue regeneration since the early 1900s. Since then, placental therapy was applied in various clinical applications as they were shown to exhibit remarkable therapeutic attributes ranging from anti-oxidant, antimicrobial, anti-inflammatory, pain reduction, hair growth promotion, health improvement, cellular proliferation, tissue regeneration and wound healing properties in vivo. This review further summarizes the historical aspects, cellular mechanism, nutrient content and therapeutics properties of the placenta.

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“…It was obtained from fish for human consumption by using Mastelli's peculiar methods, by means of purification and high-temperature sterilization procedures that ensure a very high percentage of purified DNA and the absence of active proteins and peptides [23].…”

Section: Pdrnmentioning

confidence: 99%

Anti Inflammatory Property of PDRN—An <i>in Vitro</i> Study on Cultured Macrophages

Castellini¹,

Belletti²,

Govoni³

et al. 2017

ABB

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Skin aging and most age-related diseases are associated with a low-grade chronic inflammation. The nucleoside adenosine, a potent endogenous antiinflammatory agent, is deeply involved in inflammatory diseases and, by interaction with the adenosine A 2 receptor (A 2A R) it immediately promotes a mechanism of defence against the inflammatory damage. The aim of our study was to investigate whether polydeoxyribonucleotide (PDRN), a mixture of deoxyribonucleotides polymers of different lengths that like adenosine, binds the A 2A receptor, can reduce the inflammatory state in the macrophage cell line. RAW264.7, murine macrophage cells, were incubated with PDRN in the presence and in the absence of lipopolysaccaride (LPS), which was the major component of the outer membrane of gram-negative bacteria and which acted as a strong macrophage activator. We assessed the production of nitric oxide and the secretion of inflammatory mediators (i.e., TNF-α, IL-10, IL-12 and VEGF-A). Our data showed that PDRN produced a significant decrease of inflammation in macrophages pre-stimulated with LPS, assessed in terms of the nitric oxide content (p < 0.001) and cytokines secretion (p < 0.001). Moreover, PDRN stimulated the release of the vascular endothelial growth factor (VEGF-A), which promoted wound healing. Our study suggested that PDRN, by binding the A 2A receptor, contributed to a great extent towards reducing inflammation.

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Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent

Cited by 88 publications

References 9 publications

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Placental therapy: An insight to their biological and therapeutic properties

Anti Inflammatory Property of PDRN—An <i>in Vitro</i> Study on Cultured Macrophages

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Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent

Cited by 88 publications

References 9 publications

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Placental therapy: An insight to their biological and therapeutic properties

Anti Inflammatory Property of PDRN—An &lt;i&gt;in Vitro&lt;/i&gt; Study on Cultured Macrophages

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Anti Inflammatory Property of PDRN—An <i>in Vitro</i> Study on Cultured Macrophages