Characterization and Identification of Cryptic Biopeptides inCarya illinoinensis(Wangenh K. Koch) Storage Proteins

Abstract

The objective of this research was to identify and characterize the encoded peptides present in nut storage proteins of Carya illinoinensis. It was found, through in silico prediction, proteomic analysis, and MS spectrometry, that bioactive peptides were mainly found in albumin and glutelin fractions. Glutelin was the major fraction with ~53% of the nut storage proteins containing at least 21 peptides with different putative biological activities, including antihypertensives, antioxidants, immunomodulators, protease inhibitors, and inhibitors of cell cycle progression in cancer cells. Data showed that using 50 μg/mL tryptic digests of enriched peptides obtained from nut glutelins is able to induce up to 19% of apoptosis in both HeLa and CasKi cervical cancer cells. To our knowledge, this is the first report that shows the potential value of the nut-encoded peptides to be considered as adjuvants in cancer therapies.

Quantitative In Silico Evaluation of Allergenic Proteins from Anacardium occidentale, Carya illinoinensis, Juglans regia and Pistacia vera and Their Epitopes as Precursors of Bioactive Peptides

“…Detailed review about biologically active peptides from walnut has been recently published [ 13 ]. Cashew ( Anacardium occidentale ), pecan ( Carya illinoinensis ), and pistachio ( Pistacia vera ) also are known as sources of peptides possessing various biological activities [ 24 , 25 , 26 ]. Known peptides isolated form cashew and pistachio revealed ACE inhibitory activity [ 24 , 25 ].…”

“…Known peptides isolated form cashew and pistachio revealed ACE inhibitory activity [ 24 , 25 ]. Peptides identified in the pecan hydrolysates represent diverse biological activities including ACE and DPPIV inhibition, antioxidative, anticancerogenic and other activities [ 26 ].…”