Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits

Auesukaree, Choowong; Koedrith, Preeyaporn; Saenpayavai, Pornpon; Asvarak, Thipa; Benjaphokee, Suthee; Sugiyama, Minetaka; Kaneko, Yoshinobu; Harashima, Satoshi; Boonchird, Chuenchit

doi:10.1016/j.jbiosc.2012.03.012

Cited by 47 publications

(37 citation statements)

References 32 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, the expression level of cdc encoding pyruvate kinase, which is involved in ATP production, in SC90 was greater than that in DBKKU Y-53, suggesting that SC90 required more ATP for growth and ethanol production at high temperature. The results in this study were in good agreement with Auesukaree et al [20], who observed high expression levels of genes encoding the small HSP, HSP70, HSP90, and HSP100 family and those genes encoding trehalose-6-phosphate synthase, neutral trehalase, and glycogen synthase in S. cerevisiae after heat shock and long-term heat exposure at 37˝C. Piper et al [68] reported that several HSPs are constitutively expressed at appropriate temperatures and play a crucial role in folding and assembling proteins.…”

Section: Real-time Rt-pcr Analysis Of Gene Expressionsupporting

confidence: 93%

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

et al. 2016

View full text Add to dashboard Cite

Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37˝C and 40˝C under the optimum cultural condition were 106.82 g¨L´1 and 85.01 g¨L´1, respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g¨L´1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40˝C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved.

show abstract

Section: Real-time Rt-pcr Analysis Of Gene Expressionsupporting

confidence: 93%

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

et al. 2016

View full text Add to dashboard Cite

show abstract

“…cerevisiae strains used in this study were laboratory strain W303 ( MATa/MATa ade2 - 1/ade2 - 1 his3 - 11,15/his3 - 11,15 leu2 - 3,112/leu2 - 3,112 trp1 - 1/trp1 - 1 ura3 - 1/ura3 - 1 ) (Kurtzman and Robnett 1998), industrial ethanol-producing strain TISTR5606 obtained from the Thailand Institute of Scientific and Technological Research, and the thermotolerant strains C3225, C3253, C3723, C3751, C3867, C3891, C4275, and C4377 (Auesukaree et al 2012) obtained from the yeast collection of the Department of Biotechnology, Faculty of Science, Mahidol University. YPDA (1% yeast extract, 2% peptone, 2% glucose, and 0.04% adenine) and YPDA10 (YPDA medium containing 10% glucose) media were prepared as described previously (Benjaphokee et al 2012; Burke et al 2000).…”

Section: Methodsmentioning

confidence: 99%

“…Each RNA sample was converted to cDNA by using iScript™ cDNA synthesis kit with reverse transcriptase (Bio-Rad, Hercules, CA, USA). Quantitative RT-PCR experiments were performed on a LightCycler Real-Time PCR (Roche, Mannheim, Germany) using KAPA SYBR FAST qPCR kit (Kapa Biosystems, Wilmington, MA, USA) and 200 nM specific primer pairs (SSA4-1088F and SSA4-1150R for SSA4 gene, HSP82-387F and HSP82-465R for HSP82 gene, and ACT1-458F and ACT1-529R for ACT1 gene) as described previously (Auesukaree et al 2012). Relative gene expression was calculated using the 2 − ΔΔCT method and normalized to ACT1 mRNA levels.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous report, the continuous high-level expression of heat stress-responsive genes, including those encoding heat shock proteins (HSPs) and trehalose metabolic enzymes, was shown to be involved in an acquisition of thermotolerance in S. cerevisiae (Auesukaree et al 2012). HSPs are known to function in folding unfolded and misfolded proteins, and mediating protein transport and degradation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

et al. 2016

Self Cite

View full text Add to dashboard Cite

High-temperature ethanol fermentation has several benefits including a reduction in cooling cost, minimizing risk of bacterial contamination, and enabling simultaneous saccharification and fermentation. To achieve the efficient ethanol fermentation at high temperature, yeast strain that tolerates to not only high temperature but also the other stresses present during fermentation, e.g., ethanol, osmotic, and oxidative stresses, is indispensable. The C3253, C3751, and C4377 Saccharomyces cerevisiae strains, which have been previously isolated as thermotolerant yeasts, were found to be multiple stress-tolerant. In these strains, continuous expression of heat shock protein genes and intracellular trehalose accumulation were induced in response to stresses causing protein denaturation. Compared to the control strains, these multiple stress-tolerant strains displayed low intracellular reactive oxygen species levels and effective cell wall remodeling upon exposures to almost all stresses tested. In response to simultaneous multi-stress mimicking fermentation stress, cell wall remodeling and redox homeostasis seem to be the primary mechanisms required for protection against cell damage. Moreover, these strains showed better performances of ethanol production than the control strains at both optimal and high temperatures, suggesting their potential use in high-temperature ethanol fermentation.

show abstract

“…Furthermore, the increase in global temperature in recent years has posed a serious challenge to industrial fermentation processing since abundant energy has to be spent for large cooling systems to maintain an optimum fermentation temperature. Hence, exploring and application of useful thermophilic ethanologenic microorganisms including bacteria have attracted much interests [6,7,8,9] due to their profits.…”

Section: Introductionmentioning

confidence: 99%

Screening Thermo- and Ethanol Tolerant Bacteria for Ethanol Fermentation

Dung¹,

Phong²

2013

AJMR

View full text Add to dashboard Cite

The thermophilic bacteria receive considerably interest nowadays because of a current challenge of increasing global temperature. Particularly for ethanol production, the thermo-ethanologenic bacteria possess advantages due to lower contamination risk, cost saving in industrial scale, and the wide range of sugars utilization. In this study, 13 bacterial isolates obtained from the previous isolation study were tested for their fermentative capacity and ethanol tolerance at high temperatures. Five bacterial isolates HM2, M2, MC3, MR1 and RD were found to be tolerant up to 12% ethanol. Of which HM2, M2 and MR1 could ferment glucose well at 30, 35 and 40°C, particularly isolates HM2 and MR1 could perform the fermentative capacity at 45°C and even 50°C. In the presence of 12, 16, and 20% w/v glucose, isolates HM2, M2, and MR1 showed the high fermentation rate by giving high gas production; however, the rate slightly decreased in the presence of 24% w/v glucose. The fermentative performance by these three isolates could happen at different pH levels of 4.0, 5.0 and 6.0. The favourable conditions of ethanol fermentation were found at 18.5% glucose, pH 5.0, and 33°C for isolate HM2 and at 14% glucose, pH 5.5, and 40°C for isolate MR1. The results of sequencing analysis of partial 16S rRNA gene showed that the gene sequences of the selected isolate HM2 shared 99% similarity with Bacillus subtilis.

show abstract

Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits

Cited by 47 publications

References 32 publications

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

Screening Thermo- and Ethanol Tolerant Bacteria for Ethanol Fermentation

Contact Info

Product

Resources

About