Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

Kitichantaropas, Yasin; Boonchird, Chuenchit; Sugiyama, Minetaka; Kaneko, Yoshinobu; Harashima, Satoshi; Auesukaree, Choowong

doi:10.1186/s13568-016-0285-x

Cited by 72 publications

(42 citation statements)

References 41 publications

(74 reference statements)

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“…Since some antioxidant proteins such as Gpx2p and Trx1p have been reported to be down-regulated in response to ethanol and heat stresses, respectively 25 , a decrease of intracellular antioxidants may also contribute to an increase in ROS accumulation under stress conditions. Previously, it has been shown that the synthesis of trehalose, a disaccharide involved in protecting intracellular proteins against damage and denaturation through its function as a protein stabilizer 26 , was inhibited under high glucose condition 27 , 28 . It is therefore possible that, due to a loss of trehalose-mediated protein stabilization, the high glucose stress might also cause protein denaturation, which in turn elevates intracellular ROS levels.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

Burphan

Tatip

Limcharoensuk

et al. 2018

Sci Rep

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During fermentation, yeast cells encounter a number of stresses, including hyperosmolarity, high ethanol concentration, and high temperature. Previous deletome analysis in the yeast Saccharomyces cerevisiae has revealed that SOD1 gene encoding cytosolic Cu/Zn-superoxide dismutase (SOD), a major antioxidant enzyme, was required for tolerances to not only oxidative stress but also other stresses present during fermentation such as osmotic, ethanol, and heat stresses. It is therefore possible that these fermentation-associated stresses may also induce endogenous oxidative stress. In this study, we show that osmotic, ethanol, and heat stresses promoted generation of intracellular reactive oxygen species (ROS) such as superoxide anion in the cytosol through a mitochondria-independent mechanism. Consistent with this finding, cytosolic Cu/Zn-SOD, but not mitochondrial Mn-SOD, was required for protection against oxidative stress induced by these fermentation-associated stresses. Furthermore, supplementation of ROS scavengers such as N-acetyl-L-cysteine (NAC) alleviated oxidative stress induced during very high gravity (VHG) fermentation and enhanced fermentation performance at both normal and high temperatures. In addition, NAC also plays an important role in maintaining the Cu/Zn-SOD activity during VHG fermentation. These findings suggest the potential role of ROS scavengers for application in industrial-scale VHG ethanol fermentation.

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Section: Discussionmentioning

confidence: 99%

Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

Burphan

Tatip

Limcharoensuk

et al. 2018

Sci Rep

Self Cite

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“…Up-regulation of heat stress response genes, such as LRE1 , WSC1 , SGT2 , and a variety of heat shock proteins, was observed in all samples in response to ethanol. In stress-tolerant S. cerevisiae strains, intracellular trehalose accumulates and heat shock protein genes are continuously induced in response to stresses that damage proteins, including heat, ethanol, osmotic, and oxidative stress (Kitichantaropas et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…250 µL extract was incubated with 1 mL 80% sulfuric acid solution containing 0.2% anthrone in a boiling water bath for 5 min. Absorbance at 620 nm was measured and compared with samples containing known concentrations of trehalose (Sigma-Aldrich) (Kitichantaropas et al 2016 ; Mahmud et al 2009 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

Miao

Xiong

et al. 2018

AMB Expr

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Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v). Annotation and statistical analyses suggest that multiple genes involved in ergosterol biosynthesis, trehalose metabolism, and stress response are differentially expressed under these conditions. The up-regulation of molecular chaperones HSP90 and HSP70, and also genes associated with the ubiquitin–proteasome proteolytic pathway suggests that ethanol stress may cause aggregation of misfolded proteins. Finally, ethanol stress in I. orientalis appears to have a nitrogen starvation effect, and many genes involved in nutrient uptake were up-regulated.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0568-5) contains supplementary material, which is available to authorized users.

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“…In addition to the clinical interest of these studies, they could also be relevant for biotechnological purposes derived from the interest in the optimization of fermentation processes [ 27 , 93 ] or production of cell wall polysaccharides (i.e., glucans, mannoproteins, chitin) for functional foods and pharmaceutical and cosmetic purposes [ 94 ].…”

Section: Perspectivesmentioning

confidence: 99%

The CWI Pathway: Regulation of the Transcriptional Adaptive Response to Cell Wall Stress in Yeast

Sanz

García

Rodrı́guez-Peña

et al. 2017

JoF

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Fungi are surrounded by an essential structure, the cell wall, which not only confers cell shape but also protects cells from environmental stress. As a consequence, yeast cells growing under cell wall damage conditions elicit rescue mechanisms to provide maintenance of cellular integrity and fungal survival. Through transcriptional reprogramming, yeast modulate the expression of genes important for cell wall biogenesis and remodeling, metabolism and energy generation, morphogenesis, signal transduction and stress. The yeast cell wall integrity (CWI) pathway, which is very well conserved in other fungi, is the key pathway for the regulation of this adaptive response. In this review, we summarize the current knowledge of the yeast transcriptional program elicited to counterbalance cell wall stress situations, the role of the CWI pathway in the regulation of this program and the importance of the transcriptional input received by other pathways. Modulation of this adaptive response through the CWI pathway by positive and negative transcriptional feedbacks is also discussed. Since all these regulatory mechanisms are well conserved in pathogenic fungi, improving our knowledge about them will have an impact in the developing of new antifungal therapies.Keywords: cell wall integrity; mitogen-activated protein kinase (MAPK); signal transduction; transcription; gene expression; antifungal Cell Wall Organization and Structure in S. cerevisiaeYeast cell integrity depends on the cell wall, an essential structure necessary not only for maintaining morphology but also for protecting cells against environmental stress conditions [1,2]. The cell wall is a macromolecular complex mainly composed of β-1,3-glucan, β-1,6-glucan, chitin and mannoproteins. Chitin, although a minor component, is essential for cell survival. β-1,3-glucan is the most abundant component of the yeast cell wall and serves as a backbone to which the other cell wall components are linked. The reducing ends of β-1,6-glucan and chitin are attached to the non-reducing end of β-1,3-glucan chains by an uncharacterized link and a β-1,4-linkage, respectively [3][4][5]. β-1,6-glucan is also attached to the chitin through β-1,3-linked oligogluco-residues that branch off the glucan. There is also a fraction of free chitin. Cell wall mannoproteins, including those involved in adhesion, cell wall remodeling, structural proteins and somatic antigens [6][7][8][9] can be linked directly to the β-1,3-glucan via alkali-labile bonds [9,10] and indirectly via β-1,6-glucan through a glycosylphosphatidylinositol (GPI) anchor [9,11]. The structure of the fungal cell wall is very well conserved among fungi despite several differences in cell wall composition exist [12]. Among fungal glucans, diversities in configuration, position of glyosidic bonds and branching have been reported [13]. β-1,3-glucan is the major cell wall component and is present in all the fungal species analyzed, whereas other polysaccharides including β-1,3/β-1,4-glucan, β-1,6-glucan or α-1,3-glucan a...

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Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation

Cited by 72 publications

References 41 publications

Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

Transcriptome profiling of Issatchenkia orientalis under ethanol stress

The CWI Pathway: Regulation of the Transcriptional Adaptive Response to Cell Wall Stress in Yeast

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