Characterization and control of biofilms of Salmonella Minnesota of poultry origin

Brasão, Silvia Cassimiro; Melo, Roberta Torres de; Prado, Renata Rezende; Monteiro, Guilherme Paz; Santos, Fernanda Aparecida Longato dos; Braz, Raquelline Figueiredo; Rossi, Daise Aparecida

doi:10.1016/j.fbio.2020.100811

Cited by 4 publications

(4 citation statements)

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“…The avr A, sod C, inv A, and lux S genes and resistance to sodium hypochlorite and peracetic acid in biofilms were common. These data indicate that the maintenance of these microorganisms in sessile form and contamination of the final product may be caused by cross-contamination [ 11 ].…”

Section: Resultsmentioning

confidence: 99%

“…Minnesota has been prominent among serovars due to the increasing number of isolations in the poultry production chain, prompting research to discover its danger as an agent of human salmonellosis. In Brazil, this serovar was of the second highest incidence between 2007 and 2011 in an investigation of 12,582 strains obtained from chicken carcasses and poultry products [3,11,12].…”

Section: Introductionmentioning

confidence: 99%

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Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

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The presence of virulence genes, phylogenetic relationships, biofilm formation index (BFI), and ultrastructure in S. Minnesota at different temperatures (4, 25, and 36 °C) were analyzed. In addition, the ability of biocidal agents (chlorhexidine1%, sodium hypochlorite 1%, and peracetic acid 0.8%) to inhibit biofilms formed by 20 strains isolated from broiler slaughter plants from two Brazilian companies in 2009, 2010, and 2014 was determined. The presence of specific genes was evaluated by PCR and phylogeny between strains by pulsed-field gel electrophoresis. The BFI was determined using tryptone soy broth with 5% of chicken juice, and its structure was observed by scanning electron microscopy. The presence of specific genes indicated that S. Minnesota has the potential to cause disease in humans, adapting to adverse conditions. Temperatures of 25 and 36 °C favored biofilm formation, although at 4 °C, there was still biomass that could contaminate the final product. Tolerance to all biocides was identified in 12/20 (60%), representing a real risk of adaptation mechanisms development, especially regarding to resistance to sodium hypochlorite. Phylogenetic analysis indicated cross-contamination and spread among companies, which was probably related to biofilms formation. Results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite, including the need for rigorous control, adopting low temperatures to prevent biofilms formation in the poultry industry.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

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“…(2019) in Salmonella . Especially for polyurethane, some studies have demonstrated its anti-biofilm effect on Salmonella , S. aureus and P. aeruginosa ( Nazli et al., 2019 ; Brasão et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…As the cell surface can be modulated by extrinsic characteristics and intrinsic factors, it is possible that exposure to the different conditions tested promoted a variation in hydrophobicity, altering the bacterial adhesion capacity, as identified by Moraes et al (2019) in Salmonella. Especially for polyurethane, some studies have demonstrated its anti-biofilm effect on Salmonella, S. aureus and P. aeruginosa (Nazli et al, 2019;Brasão et al, 2021).…”

Section: Effect Of Antimicrobials On Biomassmentioning

confidence: 99%

Antibiotic Resistance in the Alternative Lifestyles of Campylobacter jejuni

Rossi

Dumont

Santos

et al. 2021

Front. Cell. Infect. Microbiol.

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Campylobacter jejuni is the main pathogen identified in cases of foodborne gastroenteritis worldwide. Its importance in poultry production and public health is highlighted due to the growing antimicrobial resistance. Our study comparatively investigated the effect of five different classes of antimicrobials on the planktonic and biofilm forms of 35 strains of C. jejuni with high phylogenetic distinction in 30 of them. In the planktonic form, the existence of susceptible strains to colistin (7/35 – 20%) and resistance to meropenem (3/35 – 8.6%) represent a novelty in strains evaluated in Brazil. In biofilms formed with the addition of chicken juice, the number of resistant strains was significantly higher for colistin, erythromycin and meropenem (100%), but the susceptibility to tetracycline was shown as a control strategy for specific cases. High concentrations (1,060 ± 172.1mg/L) of antibiotics were necessary to control the biofilm structure in susceptible strains in the planktonic form, which is consistent with the high biomass produced in these strains. Stainless steel and polyurethane were the most (BFI=2.1) and least (BFI=1.6) favorable surfaces for the production of biomass treated with antimicrobials. It is concluded that the antimicrobial action was detected for all tested drugs in planktonic form. In sessile forms, the biomass production was intensified, except for tetracycline, which showed an antibiofilm effect.

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Genotype-phenotype evaluation of the heterogeneity in biofilm formation by diverse Bacillus licheniformis strains isolated from dairy products

Fan,

Liu,

Dai

et al. 2024

International Journal of Food Microbiology

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Characterization and control of biofilms of Salmonella Minnesota of poultry origin

Cited by 4 publications

References 71 publications

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Antibiotic Resistance in the Alternative Lifestyles of Campylobacter jejuni

Genotype-phenotype evaluation of the heterogeneity in biofilm formation by diverse Bacillus licheniformis strains isolated from dairy products

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