Characterization and Antibacterial Activity Test of Hydroxyapatite (HAp) from Chicken Eggshell against Lactobacillus acidophilus Bacteria

Abstract

ABSTRAKTujuan penelitian ini adalah mengkarakterisasi biokeramik Hidroksiapatit (HAp) yang disintesa dari kerabang telur ayam kampung dan kerabang telur ayam broiler menggunakan FTIR. Selain itu juga menentukan aktivitas antibakteri HAp dengan kandungan kalsium dan fosfat tertinggi (HAp dari kerabang telur ayam broiler) menggunakan metode cakram kertas. Hasil karakterisasi HAp dari kerabang telur ayam kampung menggunakan FTIR menunjukkan puncak untuk gugus fungsi PO4 3-pada panjang gelombang 1040,08 cm -1 , 608,46 cm -1 dan 568,67 cm -1 ; gugus OH -pada panjang gelombang 3426,39 cm -1 dan 2880,46 cm -1 ; gugus CO2 pada panjang gelombang 2142,91 cm -1 ; dan gugus CO3 2-pada panjang gelombang 1653,64 cm -1 . Sedangkan untuk HAp dari kerabang telur ayam broiler adalah gugus PO4 3-pada panjang gelombang 1044,40 cm -1 , 604,13 cm -1 dan 568,66 cm -1 ; gugus OH -pada panjang gelombang 3419,61 cm -1 dan 2838,35 cm -1 ; gugus CO2 pada panjang gelombang 2086,69 cm -1 ; dan gugus CO3 2-pada panjang gelombang 1653,64 cm -1 . Antibakteri HAp terhadap bakteri karies L. acidophilus memberikan hasil yang positif baik pada dosis uji terendah (5μg/μL) maupun dosis uji tertinggi (75μg/μL), ditandai dengan terbentuknya daerah daya hambat sebesar 22,93±0,066 mm dan 33,08±0,031 mm (kategori kuat yaitu >20 mm). Kesimpulan dari penelitian ini adalah hasil karakterisasi baik HAp dari kerabang telur ayam kampung maupun kerabang telur ayam broiler adalah benar senyawa HAp, dan hasil uji antibakteri dari HAp hasil sintesa kerabang telur ayam broiler menunjukkan daya antibakteri yang positif dan kuat. ABSTRACTThe purposes of this study were to characterize Hydroxyapatite (HAp) bioceramics which synthesized from local chicken and broiler eggshell using FTIR, and to determine HAp antibacterial activity with the highest content of calcium-phosphate (HAp from broiler chicken eggshell) using paper disc method. Characterization of HAp from local chicken eggshell using FTIR showed peaks for PO4 3-functional group on the wavelength 1040.08, 608.46 and 568.67 cm -1 ; OH -functional group on the wavelength 3426.39 and 2880.46 cm -1 ; CO2 functional group on the wavelength 2142.91 cm -1 ; and CO3 2-functional group on the wavelength 1653.64 cm -1 . Whereas broiler chicken eggshell HAp showed peaks for PO4 3-functional group on the

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

“…The MIC and MBC values in both the treatment group and the positive control group showed significant differences from the negative control group because the treatment given was only the basic formula without the addition of nano-hydroxyapatite. The results are in accordance with the results of Nambiar, et al and Wadu et al, 26 which indicates that the basic formula does not have antibacterial ability. 18 The limitation of this research was that it was carried out in vitro under controlled conditions.…”

Synthesis of porous Hydroxyapatite (HAp) from Laying Chicken Waste Eggshells

“…Based on equation 1 and 2 can conclude that the Ca contains in the substrates (raw materials / eggshells) [35][36][37][38][39][40][41] necessary in the HAp synthesis process to build the HAp structure [24,26,30,34]. Along the synthetic process of HAp, the main minerals from eggshells reduced [12,[42][43][44][45][46], the Ca get 66,8% reduction and leaving ±0,73-1,82% and PO4 get 2,41% reduction then leaving ±1,09-2,42% containing in HAp. The reduction of minerals is mainly caused by the incineration process using the temperatures 900-1100 o C along 3 hours.…”

“…The next steps of characterization was the FTIR scanning to the eggshells and the HAp, the chemical structure change can be described by the FTIR sketch [42,43,46,47]. The FTIR sketch of the HAp and the shells showed the differences in mineral positions mainly PO4 and CO3.…”

Hydroxiapatite (HAp) Bioceramics Made from The Caletaiya presclupta Snail Shells from Poso Lake

“…The C. persclupta shells' chemical characterization included water contains, ash, organics, carbons, and silicon by gravimetric [19]. The Phosphor has measurements by UV-Vis Spectrophotometer and HACH photometer, calcium measured twice by titrimetric [19] and UV-Vis Spectrophotometer [16], also the potassium and sulfur by HACH photometer.…”

“…The Chemical Structure of HAp [6,7] The hydroxyapatite was a product of the modification structure of calcium carbonate (CaCO3), so it can be synthesized from the rich calcium contain materials [1,4,5]. Another synthesis research before in Indonesia used several kinds of calcium source as cow bones [3,8], Molluscan shells [9,10], eggshells [11,12,13,14], Ca and P ions in bacterial cellulosic membranes [15], and calcite [16].…”