Characteristics of functionalized nano-hydroxyapatite and internalization by human epithelial cell

Abstract

Hydroxyapatite is the main inorganic component of biological bone and tooth enamel, and synthetic hydroxyapatite has been widely used as biomaterials. In this study, a facile method has been developed for the fabrication of arginine-functionalized and europium-doped hydroxyapatite nanoparticles (Arg-Eu-HAP). The synthesized nanoparticles characterized by transmission electron microscopy, X-ray diffractometry, Fourier transform infrared, and Zeta potential analyzer. Its biological properties with DNA binding, cell toxicity, cell binding and intracellular distribution were tested by agarose gel electrophoresis assay, flow cytometry, and fluorescence microscope and laser scanning confocal microscope. The synthesized Arg-Eu-HAP could effectively bind DNA without any cytotoxicity and be internalized into the cytoplasm and perinuclear of human lung epithelial cells.

A novel method for genetic transformation of C. albicans using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle

“…The bands of NH 2 and amide I associated with arginine were observed at $1448 and $1644 cm À1 , respectively. 26,32,33,39,40 The bands corresponding to -CH of glucose and C-O-C stretching bands of PEG were observed at $2943 cm À1 and $1100 cm À1 respectively (Fig. 1c).…”

“…The increase in the zeta value noted aer the functionalization of HAp could be attributed to the binding of the a-carboxylate group of arginine and OH À ions of PEG to calcium ions of HAp such that the positively charged amino groups of arginine and H + ions of PEG are exposed at the NP/solvent interface thereby increasing the zeta potential values. 26,40 A decrease in the negative surface charge aer functionalization with arginine/PEG has been observed in many NPs such as gold, silica, Fe 3 O 4 .etc. [42][43][44][45]…”

“…Hydroxyapatite nanoparticles (HAp NPs) being biocompatible are good candidates for DNA delivery and have been explored as carriers in animal and plant cells. [23][24][25][26] HAp also has advantages as it exhibits high affinity to nucleic acids and can be easily transported through cell membranes. [23][24][25][26] In this work, we have synthesized arginine (R), glucose (G) and polyethylene glycol (PEG) functionalized thin-rod HAp NPs (referred to as M-HAp NPs) which form stable complexes with pDNA.…”

Calcium orthophosphate bioceramics

“…Additionally, other investigators indicated to mechanical properties increasing for other CaPO 4 scaffolds after induced osteogenesis[589,592].To finalize this section, one should mention on the recent developments to influence the cellular response. First, to facilitate interactions with cells, the CaPO 4 surfaces could be functionalized[757][758][759][760][761]. Second, it appears that crystals of biological apatite of calcified tissues exhibit different orientations depending on the tissue.…”

Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model

“…Sun et al 28,29 successfully used nHAP to deliver the NT-3 gene into the cochlear neurons of guinea pigs both in vitro and in vivo, and further demonstrated that surface modification of nHAP with polyethylenimine carrying specific genetic materials could pass through the intact round window membrane of the chinchilla with high transfection efficiency and low toxicity. Yan-Zhong et al 30 used arginine-modified nanohydroxyapatite to change the surface charge of nHAP, thereby improving adsorption capacity in human epithelial cells. These studies demonstrate that nHAP may be a potentially effective and safe gene vector with possible clinical application.…”