Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model

Chen, Yan; Yang, Lifang; Huang, Suping; Li, Zhi; Zhang, Lu; Jiang, He; Xu, Zhijie; Liu, Liyu; Cao, Yang; Sun, Lun‐Quan

doi:10.2147/ijn.s48321

Cited by 7 publications

(2 citation statements)

References 44 publications

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“…Recently, nano-sized HA (nHA) particles have also been tested as a potential biomaterial for drug delivery. An arginine-modified nHA delivery system has previously been developed and demonstrated to successfully deliver intracellular DNAzymes in both in vitro cell lines and in vivo mouse xenograft (5–7).…”

Section: Introductionmentioning

confidence: 99%

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway

Guo

Tian

et al. 2019

Exp Ther Med

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Nano-sized hydroxyapatite (nHA) particles have been demonstrated to exert anti-cancer effects on multiple cancer cell lines and animal models of cancer biology. However, the molecular mechanism underlying the effects of nHA particles on glioma cells remains unclear. The present study aimed to examine the effects of nHA on the behavior of glioma cells and investigate its underlying molecular mechanism. Rat glioma C6 cells and human glioma U87MG ATCC cells were exposed to nHA (20–100 µg/ml), and its effects on cell morphology, viability, apoptosis, cell cycle, invasion and nuclear factor (NF)-κB signaling were analyzed. Exposure of C6 and U87MG ATCC cells to 20 µg/ml nHA for 24 h caused cell detachment. Viability of C6 and U87MG ATCC cells were significantly reduced by nHA in a dose-dependent manner (P<0.05). Nuclear staining with Hoechst 33258 exhibited clear chromatin condensation in C6 cells following 24 h exposure to ≥25 µg/ml nHA. Flow cytometry revealed that nHA (20–100 µg/ml) significantly induced apoptosis and cell cycle G2/M arrest in C6 and U87MG ATCC cells (P<0.05). Transwell invasion assay demonstrated that nHA (20–60 µg/ml) significantly inhibited invasion of U87MG ATCC cells (P<0.05). Furthermore, western blotting and confocal immunofluorescence microscopy revealed that nHA (20–100 µg/ml) decreased NF-κB p65 protein expression and blocked NF-κB p65 nuclear translocation in C6 cells. The protein expression of NF-κB target molecules, such as B cell lymphoma 2, cyclooxygenase-2 and survivin, were also significantly reduced by nHA in a dose-dependent manner in both C6 and U87MG ATCC cells (P<0.05). In conclusion, it was demonstrated that the inhibitory effect of nHA on glioma cells is likely associated with the downregulation of NF-κB signaling.

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Section: Introductionmentioning

confidence: 99%

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway

Guo

Tian

et al. 2019

Exp Ther Med

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“…In addition to iron oxide rice particles, arginine-modified nano-hydroxyapatite particles (Arg-nHAP) had been confirmed to be used to deliver Dz1 in the CNE1-LMP1 xenotransplantation model of mice. Arg-nHAP and Dz1 had high transfection efficiency under in vitro conditions, and the use of specific inhibitors confirmed that the cell uptake of Arg-nHAP and Dz1 complex was mediated by energy-dependent endocytosis, The confocal microscope observed effective intracellular delivery and nuclear localization of the complex and also significantly downregulated the expression of LMP1 in nasopharyngeal carcinoma cells and inhibited tumor growth in mice ( Chen et al, 2013 ). In addition, Levina et al ( Levina et al, 2014 ) coupled DNAzymes with titanium oxide nanoparticles.…”

Section: Delivery Strategy Of Dnazymesmentioning

confidence: 90%

A promising nucleic acid therapy drug: DNAzymes and its delivery system

Xiao,

Zhao,

Yang

et al. 2023

Front. Mol. Biosci.

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Based on the development of nucleic acid therapeutic drugs, DNAzymes obtained through in vitro selection technology in 1994 are gradually being sought. DNAzymes are single-stranded DNA molecules with catalytic function, which specifically cleave RNA under the action of metal ions. Various in vivo and in vitro models have recently demonstrated that DNAzymes can target related genes in cancer, cardiovascular disease, bacterial and viral infection, and central nervous system disease. Compared with other nucleic acid therapy drugs, DNAzymes have gained more attention due to their excellent cutting efficiency, high stability, and low cost. Here, We first briefly reviewed the development and characteristics of DNAzymes, then discussed disease-targeting inhibition model of DNAzymes, hoping to provide new insights and ways for disease treatment. Finally, DNAzymes were still subject to some restrictions in practical applications, including low cell uptake efficiency, nuclease degradation and interference from other biological matrices. We discussed the latest delivery strategy of DNAzymes, among which lipid nanoparticles have recently received widespread attention due to the successful delivery of the COVID-19 mRNA vaccine, which provides the possibility for the subsequent clinical application of DNAzymes. In addition, the future development of DNAzymes was prospected.

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Co-infection of Epstein-Barr virus and human papillomavirus in human tumorigenesis

et al. 2016

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Viral infections contribute to approximately 12% of cancers worldwide, with the vast majority occurring in developing countries and areas. Two DNA viruses, Epstein-Barr virus (EBV) and human papillomavirus (HPV), are associated with 38% of all virus-associated cancers. The probability of one patient infected with these two distinct types of viruses is increasing. Here, we summarize the co-infection of EBV and HPV in human malignancies and address the possible mechanisms for the co-infection of EBV and HPV during tumorigenesis.

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Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model

Cited by 7 publications

References 44 publications

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway

A promising nucleic acid therapy drug: DNAzymes and its delivery system

Co-infection of Epstein-Barr virus and human papillomavirus in human tumorigenesis

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