Characteristics and Epidemiologic Genotyping of Staphylococcus aureus Isolates from Bovine Mastitic Milk in Hokkaido, Japan

Hata, Enjô; Katsuda, Ken; Kobayashi, Hideki; Ogawa, Torata; Endô, Takayuki; Eguchi, Masashi

doi:10.1292/jvms.68.165

Cited by 35 publications

(35 citation statements)

References 28 publications

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“…Though the 88 S. aureus isolated between 2010 and 2012 belong to 14 different pulsotypes, 3 of them (A, B, D) group together 72 % and 5 of them (A, B, D, N, Z), 83 % of the isolates, confirming, like in other studies, that a restricted number of S. aureus clones are present within a herd or a farm (Hata et al 2006;Aires-de-Sousa et al 2007;Rabello et al 2007;Anderson et al 2012). Nevertheless, apart from the A and B pulsotypes (>90 % similarity), the other pulsotypes are not closely related and represent distinct lineages (Fig.…”

Section: Discussionsupporting

confidence: 81%

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

Issa

Duprez

Alambédji

et al. 2015

Trop Anim Health Prod

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Staphylococcus (S.) aureus is one of the most important pathogens causing bovine mastitis. The aim of the present work was to follow in three herds and during the 3 years the clonality of S. aureus isolated from California Mastitis Test (CMT)-positive cows at the experimental station of Toukounous (Niger) by (i) comparing their pulsed field gel electrophoresis (PFGE) fingerprints, (ii) identifying their virulotypes by PCR amplification and (iii) assessing the production of capsule and the formation of biofilm. The 88 S. aureus isolates belonged to 14 different pulsotypes, 3 of them being predominant: A (30 %), D (27 %), B (15 %). A and B pulsotypes had the highest profile similarity coefficient (94 %), while others had similarity coefficients under 60 %. Seventy-five S. aureus isolates were further studied for their virulotypes, capsular antigens and biofilm production. Most surface factor-, leukocidin-and haemolysin-, but not the enterotoxin-encoding genes were detected in the majority (>75 %) of the isolates and were evenly distributed between the A, B and D pulsotype isolates. The majority of the 72 S. aureus positive with the cap5H or cap8H PCR produced the CP5 (82 %) or the CP8 (88 %) capsular antigen, respectively. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates, moderate for 31 isolates but weak for 18 isolates, implying that the icaA gene may not be expressed in vitro by one third of the positive isolates. Similar to other studies, those results confirm that a restricted number of S. aureus clones circulate within the three herds at Toukounous and that their specific virulence-associated properties must still be further studied.

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Section: Discussionsupporting

confidence: 81%

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

Issa

Duprez

Alambédji

et al. 2015

Trop Anim Health Prod

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“…1 and Table 2). The majority of seg and sei distribution studies show that these genes are always detected together in S. aureus because they coexist on a common genetic element, a so-called enterotoxin gene cluster (Jarraud et al, 2001;Rosec and Gigaud, 2002;Stephan et al, 2002;Scherrer et al, 2004;Katsuda et al, 2005;Bania et al, 2006;Boerema et al, 2006;Hata et al, 2006;Kérouanton et al, 2007). It should also be noted that none of our isolates possessed the see, seh, sej, or tst genes, and on 15 out of 20 farms tested, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk (Table 2).…”

Section: Pcr Amplification Of Genes Encoding Staphylococcal Enterotoxinsmentioning

confidence: 99%

“…Traditionally, five classical antigenic SE types (SEA, SEB, SEC, SED, and SEE) were recognized. However, in recent years, the existence of new types of SEs, including enterotoxin-like (SEl) toxins (SEG, SEH, SEI, SElJ, SElK, SElL, SElM, SElN, SElO, SElP, SElQ, SElR, SElU, SElU2, and SElV) has been reported and their genes described (Letertre et al, 2003;Lina et al, 2004;Omoe et al, 2004;Jørgensen et al, 2005a;Bania et al, 2006;Boerema et al, 2006;Hata et al, 2006;Thomas et al, 2006). A distantly related protein, toxic shock syndrome toxin 1 (TSST-1), also produced by S. aureus, was the first toxin shown to be involved in the toxic shock syndrome of humans and animals (Akineden et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary

Peles

Wagner

Varga

et al. 2007

International Journal of Food Microbiology

132

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Staphylococcus aureus is a major foodborne pathogen due to its capability to produce a wide range of heat-stable enterotoxins. The primary purpose of this research was to characterize S. aureus isolates recovered from mammary quarter milk of mastitic cows and from bulk tank milk produced on Hungarian dairy farms of different sizes. Macrorestriction analysis of chromosomal DNA from S. aureus isolates was performed using the restriction enzyme SmaI followed by pulsed-field gel electrophoresis (PFGE). The prevalence rates of nine S. aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and of the toxic shock syndrome toxin 1 gene (tst) were determined by multiplex polymerase chain reaction (PCR). The bulk tank milks of 14 out of 20 farms were contaminated with S. aureus at levels of up to 6.0 × 10 3 CFU/ml. Farm size had no significant effect (P N 0.05) on the S. aureus counts in bulk milk. The prevalence rates of penicillin resistance were 88.9% and 20.0% among the S. aureus recovered from mastitic quarter milk and bulk tank milk, respectively. After phenotypic characterization, a total of 59 S. aureus isolates were selected for genotyping. PFGE analysis revealed 22 distinct pulsotypes, including 14 main types and 8 subtypes, at a similarity level of 86%. Only one or two main types were observed on each of the farms tested, indicating a lack of genetic diversity among S. aureus isolates within farms, and there were only two pulsotypes which occurred on more than one farm. The PFGE patterns showed genetic relatedness between the S. aureus strains recovered from quarter milk and bulk milk on two large farms, implying that on farms having a high number of mastitic cows, S. aureus from infected udders may contaminate bulk milk and, subsequently, raw milk products. Sixteen (27.1%) of the S. aureus isolates tested by multiplex PCR were found to be positive for enterotoxin genes, with 15 of them carrying just one gene and one strain carrying two genes (seg and sei). The most commonly detected toxin genes were seb, sea, and sec, whereas none of our isolates possessed the see, seh, sej, or tst genes. On 75% of the dairy farms surveyed, no enterotoxigenic staphylococci were recovered from either mastitic quarter milk or bulk tank milk.

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“…A limited number of molecular studies have been published that explored the population structure and genetic relationships of S. aureus causing bovine mastitis (de Sousa et al, 2007;Hata et al, 2006;Jørgensen et al, 2005;Katsuda et al, 2005;Reinoso et al, 2008). The results of our recent study demonstrated that a Multiple Locus Variable Number Tandem Repeats (VNTR) Analysis (MLVA) could be used as a fast, inexpensive, highly discriminatory, reproducible, stable and portable typing method for epidemiological tracing of human S. aureus (Ikawaty et al, 2008).…”

Section: Introductionmentioning

confidence: 95%

Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

Ikawaty

Brouwer

Jansen

et al. 2009

Veterinary Microbiology

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Characteristics and Epidemiologic Genotyping of Staphylococcus aureus Isolates from Bovine Mastitic Milk in Hokkaido, Japan

Cited by 35 publications

References 28 publications

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary

Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

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