Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

Ikawaty, Risma; Brouwer, Eric; Jansen, Marc; Duijkeren, Engeline van; Mevius, Dik; Verhoef, J.; Fluit, Ad C.

doi:10.1016/j.vetmic.2008.10.034

Cited by 46 publications

(47 citation statements)

References 23 publications

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“…aureus strain identified in Ireland and the United States (Fitzgerald et al, 1997), later identified to be CC151 (Herron-Olson et al, 2007), was observed in our study and another study from the Netherlands (Ikawaty et al, 2009). Clonal complex CC479 was also common in that study (Ikawaty et al, 2009), as well as in our study. We identified ST50 in a single herd with high Staph.…”

Section: Discussionsupporting

confidence: 82%

“…Thirteen ST were observed, belonging to 8 CC, with 3 new ST identified. Approximately 20% of the isolates belonged to CC (CC1, CC8, CC9, and CC398) not previously associated with bovine mastitis (Smith et al, 2005b;Aires-de-Sousa et al, 2007;Rabello et al, 2007;Hasman et al, 2009;Ikawaty et al, 2009). All 34 ST50 isolates were identified in a single herd and this ST was widespread within the herd: 34 out of 40 quarters from which isolates were genotyped were positive for ST50 Staph.…”

Section: Mlstmentioning

confidence: 89%

“…Alleles, ST, and clonal complexes (CC; groups of closely related ST) were assigned using the MLST database. Bovine-associated CC were distinguished from CC associated with other hosts based on the MLST database and literature (ArmandLefevre et al, 2005;Smith et al, 2005b;Aires-de-Sousa et al, 2007;Rabello et al, 2007;Cuny et al, 2008;Deurenberg and Stobberingh, 2008;Ikawaty et al, 2009;Walther et al, 2009;Hasman et al, 2010;Weese and van Duijkeren, 2010). Clonal complexes were defined to be bovine adapted if isolates belonging to those CC were frequently found in milk samples, and included CC50, CC97, CC151, and CC479.…”

Section: Genotypingmentioning

confidence: 99%

“…Some sequence types (ST) such as ST398 are found in many host species, including humans and animals (Weese and van Duijkeren, 2010), whereas other types of Staph. aureus are specifically associated with animals (Sung et al, 2008;Ikawaty et al, 2009;Lowder et al, 2009). Bovineassociated Staph.…”

Section: Introductionmentioning

confidence: 99%

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Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Borne

Nielen

Schaik

et al. 2010

Journal of Dairy Science

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Staphylococcus aureus causes a wide range of diseases in multiple species. Some sequence types (ST) are observed in a variety of hosts, whereas other strains are mainly associated with bovine mastitis, suggesting host adaptation. We propose that host adaptation of Staph. aureus may influence bacteriological cure of bovine subclinical mastitis after antimicrobial treatment. To test this hypothesis, multilocus sequence typing was performed on Staph. aureus isolates from 60 treated and 79 untreated control quarters that were obtained from well-defined cohorts of dairy cows from a recently conducted randomized field trial on early treatment of subclinical mastitis. Bovine-associated ST were distinguished from non-bovine-associated ST based on the literature and public databases. The association between host adaptation and bacteriological cure was investigated using population-averaged logistic regression models. Thirteen ST were identified, with approximately 80% of isolates belonging to bovine-associated ST. The odds for cure were around 2.5 times as high for non-bovine-associated ST as for bovine ST in treated quarters, whereas no difference in spontaneous cure was observed in untreated control quarters. In addition, host adaptation was related to known predictors of cure, such as penicillin susceptibility and somatic cell count. All isolates belonging to non-bovine-associated ST were resistant to penicillin, whereas the majority of isolates belonging to bovine-associated ST were penicillin susceptible. Penicillin-resistant bovine-associated strains were associated with high somatic cell counts compared with other strains. The correlation between penicillin resistance, cell counts, and host adaptation may affect the association between host adaptation and cure. For diagnostic purposes, a simple and fast alternative to multilocus sequence typing of Staph. aureus to determine host adaptation may be valuable.

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Section: Discussionsupporting

confidence: 82%

Section: Mlstmentioning

confidence: 89%

Section: Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Borne

Nielen

Schaik

et al. 2010

Journal of Dairy Science

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“…All obtained isolates were screened by duplex PCR using primers specific for mecA gene and clonal complex 398 (28). Strain MRSA CC398 S0358, kindly provided by the University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary Disease and Biology, served as a positive control (14). The detection of enterotoxin genes sea, seb, sec, sed, and see was performed by the method described by Becker et al (2).…”

Section: Methodsmentioning

confidence: 99%

Characterisation of Staphylococcus aureus isolated from meat processing plants – a preliminary study

Kizerwetter-Świda

Chrobak-Chmiel

Rzewuska

et al. 2016

Journal of Veterinary Research

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Introduction: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) belonging to the clonal complex 398 (CC398) emerged recently in livestock as a new type of MRSA, which may cause zoonotic infections. This study presents data on the characterisation of S. aureus isolated from the meat processing plants. Material and Methods: S. aureus was isolated from 90 samples collected in the raw meat warehouse, from devices and surfaces of meat processing plants, and from finished meat products. The isolates were subjected to molecular analysis in order to investigate the presence of enterotoxin genes, the mecA gene, and to verify whether they belong to the clonal complex 398. The genetic relatedness of the isolates was determined using pulsed-field electrophoresis. Likewise, antimicrobial susceptibility was tested. Results: From 21 S. aureus strains isolated, five belonged to the CC398, two of which were recognised as MRSA and three as methicillin-sensitive Staphylococcus aureus (MSSA). The most prevalent enterotoxin genes were seg and sei. Two MRSA CC398 isolates, three MSSA CC398, and one MSSA were classified as multidrug-resistant. Conclusion: The first isolation of MSSA CC398 from beef in Poland indicates contamination of beef by strains belonging to this clonal complex. The occurrence of multidrug-resistant enterotoxigenic S. aureus isolates in the finished meat products constitutes a potential risk for the consumers.

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Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

Zadoks

Middleton

McDougall³

et al. 2011

J Mammary Gland Biol Neoplasia

335

275

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Mastitis, inflammation of the mammary gland, can be caused by a wide range of organisms, including gram-negative and gram-positive bacteria, mycoplasmas and algae. Many microbial species that are common causes of bovine mastitis, such as Escherichia coli, Klebsiella pneumoniae, Streptococcus agalactiae and Staphylococcus aureus also occur as commensals or pathogens of humans whereas other causative species, such as Streptococcus uberis, Streptococcus dysgalactiae subsp. dysgalactiae or Staphylococcus chromogenes, are almost exclusively found in animals. A wide range of molecular typing methods have been used in the past two decades to investigate the epidemiology of bovine mastitis at the subspecies level. These include comparative typing methods that are based on electrophoretic banding patterns, library typing methods that are based on the sequence of selected genes, virulence gene arrays and whole genome sequencing projects. The strain distribution of mastitis pathogens has been investigated within individual animals and across animals, herds, countries and host species, with consideration of the mammary gland, other animal or human body sites, and environmental sources. Molecular epidemiological studies have contributed considerably to our understanding of sources, transmission routes, and prognosis for many bovine mastitis pathogens and to our understanding of mechanisms of host-adaptation and disease causation. In this review, we summarize knowledge gleaned from two decades of molecular epidemiological studies of mastitis pathogens in dairy cattle and discuss aspects of comparative relevance to human medicine.

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Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

Cited by 46 publications

References 23 publications

Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Host adaptation of bovine Staphylococcus aureus seems associated with bacteriological cure after lactational antimicrobial treatment

Characterisation of Staphylococcus aureus isolated from meat processing plants – a preliminary study

Molecular Epidemiology of Mastitis Pathogens of Dairy Cattle and Comparative Relevance to Humans

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