Characterisation of volatile compounds in Tunisian fenugreek seeds

Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change

“…Despite the widespread use of these herbs, little is known about their safety and efficacy (Hu et al, 2009). Considering this, the GC-MS data of methanolic extract of Fenugreek has been reported that there are major classes of compounds such as aldehydes, ketones, acids, alcohols, sulfur compounds, furans, monoterpenes, sesquiterpenes, and aromatic hydrocarbons are found, which can be used as a phototherapy or chemotherapy (Mebazaa et al, 2009). The unique amino acid, 4-hydroxyisoleucine stimulates the discharge of insulin thereby controlling blood sugar levels (Gupta et al, 2001;Broca et al, 2004;Haeri et al, 2009).…”

“…It is one of the oldest medicinal plants known and has long been recognized as a traditional medicine in Asia, Africa and Mediterranean countries (Mebazaa et al, 2009;Naidu et al, 2011). Current research on Fenugreek has shown that it contains active beneficial chemical constituents, including steroidal sapogenins (Taylor et al, 1997), dietary fiber (Naidu et al, 2011), galactomannans (Wu et al, 2009), antioxidants (Naidu et al, 2011), and amino acids such as 4-hydroxyisoleucine which possess anti-diabetic (Kumar et al, 2005), hypocholesterolemic and hypoglycemic properties (Meghwal et al, 2012) which have potential to be used in the treatment of antileukemic (Acharya et al, 2011), antipyretic (Bhatia et al, 2006), antinociceptive, antifertility activity, cure leprosy galactogogue (Bhalke et al, 2009;Khoja et al, 2011), obesity, diabetes and cancer (Thomas et al, 2011).…”

Analysis of human male armpit sweat after fenugreek ingestion: Instrumental and sensory optimisation of the extraction method

“…Each gauze pad piece was soaked in 30 ml of methanol under stirring for 2 h at 25°C; the choice of methanol was based on our previous work, showing its efficiency in extracting high and medium volatile compounds (in particular sotolon) from fenugreek seeds (Mebazaa et al, 2009). The organic phase was further recovered and dried over anhydrous sodium sulphate; after filtration, the organic extract was finally reduced to a volume of 1 ml by concentration with a Vigreux column.…”

“…Then, HS-SPME under optimum conditions and solvent procedure were applied to extract compounds from the armpit sweat of the two volunteer subjects after fenugreek ingestion. Finally, the representativeness of HS-SPME and methanol extracts was sensorially evaluated by eight trained assessors, using, respectively, direct gas chromatography-olfactometry and direct olfaction as tools to evaluate the odour of the armpit sweat extracts, following a methodology largely applied in flavour analysis (Mebazaa et al, 2009;Rega, Fournier, & Guichard, 2003).…”

Applications of In Vivo and In Vitro Solid-Phase Microextraction Techniques in Plant Analysis

“…For static HS-SPME protocol, the seeds were ground and two kinds of fibers were compared. DVB/carboxen/PDMS fiber was confirmed to possess high extraction efficiency to the odorous compounds of fenugreek seeds [104]. Hashemi et al functionalized SBA-15 with 3-[bis (2-hydroxyethyl)amino]propyl-triethoxysilane and then glued the functionalized particles to copper wire to develop a new SPME fiber to study the essential oil composition of cumin seeds collected from different climates.…”