Applications of In Vivo and In Vitro Solid-Phase Microextraction Techniques in Plant Analysis

Abstract

Plant tissues usually consist of various complex matrices. Therefore, there is a clear and unmet need for the development of suitable systems for the collection and analysis of targeted analytes in plant, particularly for the sample pretreatment methods. Solid-phase microextraction (SPME) combines sampling, isolation, concentration, and enrichment in one step and is a simple and effective sample preparation technique. Due to the high-efficiency, cost-saving, solvent-free, and easy-to-realize automation properties, SPME is among the more promising and new, green sample preparation techniques for the determination of traces of different compounds of plants in the last decade, such as volatile organic compounds (VOCs) emitted from different organs of plants and a variety of environmental contaminants in plants. In this chapter, we summarize the recent applications of in vitro and in vivo SPME in different organs of plant and also discuss the advantages of the state-of-the-art SPME technology applied in the fields of plant analysis.

Analytical artefacts: H2 carrier gas hydrogenation of plant volatiles during headspace solid‐phase microextraction gas chromatography

“…7 Head space (HS) SPME is a fast sampling technique which avoids potential solvent-related artefacts such as products from solventinduced reactions, 9 and is therefore very attractive for GC analyses of plant volatiles. [10][11][12] We now report hydrogenation during HS-SPME-GC analyses of the common plant volatile classes of phenylpropanoids, monoterpenes, and green leaf volatiles (GLVs), 1 and the effects of different fibre coatings.…”