Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change

Alshatwi, Ali A.; Shafi, Gowhar; Hasan, Tarique N.; Syed, Naveed Ahmed; Khoja, Kholoud K.

doi:10.7314/apjcp.2013.14.10.5783

Cited by 36 publications

(20 citation statements)

References 40 publications

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“…In due course our group already has demonstrated the anti-preoperative potential of organic extracts from root bark of Juglans Regia against MDA-MB-231 human breast cancer cells (Hasan et al, 2011). In addition of that, our group also demonstrated that methanolic extract of fenugreek whole plant (without root) has potential to induce apoptosis in MCF-7 human breast cancer cells through the involvement of Fas receptor and Caspase-8 (Alshatwi et al, 2013). In present study we are focusing on the anti cancer and apotosis inducing ability of NS seed extract in MCF-7 human breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Maintenance and culture procedures were exactly similar to that of reported in our earlier study (Alshatwi et al, 2013). Briefly, The cell line was maintained and propagated in 90% Dulbecco's Modified Eagle's Medium (HyClone, Thermo Scientific, MA, USA) containing 10% fetal bovine serum (HyClone, Thermo Scientific) and 1% penicillin/streptomycin (HyClone, Thermo Scientific).…”

Section: Maintenance Of Mcf-7 Cellsmentioning

confidence: 99%

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Roles of p53 and Caspases in Induction of Apoptosis in MCF-7 Breast Cancer Cells Treated with a Methanolic Extract of Nigella Sativa Seeds

Alhazmi

Hasan²,

Shafi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Nigella Sativa (NS) is an herb from the Ranunculaceae family that exhibits numerous medicinal properties and has been used as important constituent of many complementary and alternative medicines (CAMs). The ability of NS to kill cancer cells such as PC3, HeLa and hepatoma cells is well established. However, our understanding of the mode of death caused by NS remains nebulous. The objective of this study was to gain further insight into the mode and mechanism of death caused by NS in breast cancer MCF-7 cells. Materials and Methods: Human breast cancer cells (MCF-7) were treated with a methanolic extract of NS, and a dose-and time-dependent study was performed. The IC 50 was calculated using a Cell Titer Blue ® viability assay assay, and evidence for DNA fragmentation was obtained by fluorescence microscopy TUNEL assay. Gene expression was also profiled for a number of apoptosis-related genes (Caspase-3, -8, -9 and p53 genes) through qPCR. Results: The IC 50 of MCF-7 cells was 62.8µL/mL. When MCF-7 cells were exposed to 50 µL/mL and 100 µL/mL NS for 24h, 48h and 72h, microscopic examination (TUNEL assay) revealed a dose-and time-dependent increase in apoptosis. Similarly, the expression of the Caspase-3, -8, -9 and p53 genes increased significantly according to the dose and time. Conclusions: NS induced apoptosis in MCF-7 cells through both the p53 and caspase pathways. NS could potentially represent an alternative source of medicine for breast cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Maintenance Of Mcf-7 Cellsmentioning

confidence: 99%

Roles of p53 and Caspases in Induction of Apoptosis in MCF-7 Breast Cancer Cells Treated with a Methanolic Extract of Nigella Sativa Seeds

Alhazmi

Hasan²,

Shafi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Crude extracts of fenugreek decreased the cell viability toward both drug-sensitive and drug-resistant cancer cell lines (Saeed et al, 2015). Methanol extracts from the whole plant induced cytotoxicity in MCF-7 breast cancer cells (IC 50 = 65 μg/mL; Alshatwi et al, 2013). In the same cell model, a chloroform extract also induced cytotoxicity (IC 50 = 41.6 μg/mL; Khoja et al, 2011).…”

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

North African Medicinal Plants Traditionally Used in Cancer Therapy

et al. 2017

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Background: Cancer is a major cause of mortality worldwide with increasing numbers by the years. In North Africa, the number of cancer patients is alarming. Also shocking is that a huge number of cancer patients only have access to traditional medicines due to several factors, e.g., economic difficulties. In fact, medicinal plants are widely used for the treatment of several pathologies, including cancer. Truthfully, herbalists and botanists in North African countries prescribe several plants for cancer treatment. Despite the popularity and the potential of medicinal plants for the treatment of cancer, scientific evidence on their anticancer effects are still scarce for most of the described plants.Objective: Bearing in mind the lack of comprehensive and systematic studies, the aim of this review is to give an overview of studies, namely ethnobotanical surveys and experimental evidence of anticancer effects regarding medicinal plants used in North Africa for cancer therapy.Method: The research was conducted on several popular search engines including PubMed, Science Direct, Scopus and Web of Science. The research focused primarily on English written papers published between the years 2000 and 2016.Results: This review on plants traditionally used by herbalists in North Africa highlights that Morocco and Algeria are the countries with most surveys on the use of medicinal plants in folk medicine. Among the plethora of plants used, Nigella sativa and Trigonella foenum-graecum are the most referred ones by herbalists for the treatment of cancer. Moreover, a plethora of scientific evidence qualifies them as candidates for further drug development. Furthermore, we report on the underlying cellular and molecular mechanisms.Conclusion: Overall, this review highlights the therapeutic potential of some medicinal plants as anticancer agents. The North African flora offers a rich source of medicinal plants for a wide array of diseases, including cancer. The elucidation of their modes of action represents an indispensable condition for the rational development of new drugs for cancer treatment. Furthermore, testing the anticancer activity in vivo and in clinical trials are warranted to explore the full therapeutic potential of North African plants for cancer therapy.

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“…In the last several years, traditional methods used to fight cancer cells have been largely based on the use of plant natural products. Recent studies have reported that natural products have positive effects against cancer cells compared with chemotherapy (Kim et al, 2012;Wang et al, 2012;Alshatwi et al, 2013;Jung et al, 2014). Therefore, the present study was aimed to investigate the in vitro cytotoxic activity of two isolates of Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

El‐Rahman

El-Shafei²,

Иванова³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-

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Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change

Cited by 36 publications

References 40 publications

Roles of p53 and Caspases in Induction of Apoptosis in MCF-7 Breast Cancer Cells Treated with a Methanolic Extract of Nigella Sativa Seeds

Roles of p53 and Caspases in Induction of Apoptosis in MCF-7 Breast Cancer Cells Treated with a Methanolic Extract of Nigella Sativa Seeds

North African Medicinal Plants Traditionally Used in Cancer Therapy

Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

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