Characterisation of spontaneous and induced epithelial and mesenchymal tumours by their enzyme patterns

Wilson, Patricia D.

doi:10.1002/path.1711130304

Cited by 5 publications

(1 citation statement)

References 26 publications

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“…1972; Siliprandi et al, 1973;W ilson, 1972Bernhard, 1969;F rolkis and Bo-GATSKAYA, 1968;G lew et al, 1973;Martin et al, 1974;W ilson, 1974], but there are few detailed studies. In this preliminary paper we review the problem and describe age-associated changes in mitochondria in the mouse liver in our colony of ageing mice.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Age on Mitochondrial Ultrastructure

Wilson

Franks²

1975

Gerontology

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The ultrastructure of perfused livers and of mitochondrial fractions from 6-month-old and 30-month-old C57/BL mice were studied. In old mice the liver cell mitochondria were enlarged and rounded with a light ‘foamy’, vacuolated matrix, short cristae and a loss of dense granules. Quantitative studies showed a 60% increase in the mean size and an increased proportion of larger mitochondria in intact 30-month-old perfused livers. Endothelial and Kupffer cell mitochondria were smaller than those of the parenchymal cells. Mitochondria in pellets prepared from 6- and 30-month-old livers were rounded and condensed, although there were a few larger and ‘foamy’ mitochondria in the preparations from old mice. Up to 47% of large mitochondria in the old livers were lost during cell fractionation.

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Section: Introductionmentioning

confidence: 99%

The Effect of Age on Mitochondrial Ultrastructure

Wilson

Franks²

1975

Gerontology

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Alkaline phosphatase in mitochondria

Wilson¹,

Franks²,

Cottell³

et al. 1977

Cell Biology International Reports

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In electron microscope cytochemical studies alkaline phosphatase activity was present in the mitochondria of all liver cells and associated with the plasma membrane of the cells of bile canaliculi. The mitochondrial activity was partially inhibited by L-phenylalanine and Levamisole but the plasma membrane associated activity was completely inhibited by Levamisole. Biochemical assays have shown that a significant amount of the total mouse liver alkaline phosphatase activity was present in the mitochondria fraction. Starch gel electrophoresis showed that this mitochondrial alkaline phosphatase had a characteristic isoenzyme pattern, consisting of 3 distinct bands which were not retarded by neuraminidase treatment. The enzyme in the mitochondria-free supernatant showed one wide band which was retarded by neuraminidase.

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Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: Not an invariable marker for neoplastic transformation

Wilson¹,

Benham²,

Franks³

1977

Cell Biology International Reports

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The cytochemical localisation and presumed isoenzyme type (based on selective inhibition experiments) of alkaline phosphatase in 5 cell lines derived frrom normal human, rat, mouse and hamster tissues, 6 human lymphoblastoid lines and 6 human and mouse tumour-derived cell lines are described. Enzyme activity varied between the cell lines. An isoenzyme inhibited by L-phenylalanine was present in 3 normal lines, 3 lymphoblastoid lines and 2 tumour lines. The presence of this isoenzyme cannot be used as a marker of neoplastic transformation.

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Characterisation of spontaneous and induced epithelial and mesenchymal tumours by their enzyme patterns

Cited by 5 publications

References 26 publications

The Effect of Age on Mitochondrial Ultrastructure

The Effect of Age on Mitochondrial Ultrastructure

Alkaline phosphatase in mitochondria

Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: Not an invariable marker for neoplastic transformation

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