Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: Not an invariable marker for neoplastic transformation

Wilson, Patricia D.; Benham, Frances; Franks, L. M.

doi:10.1016/0309-1651(77)90047-9

Cited by 14 publications

(4 citation statements)

References 12 publications

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“…invariant marker of malignancy in this experimental system. A similar conclusion has been reached from the study of a variety of different human tumor-derived cell lines (34).…”

Section: Methodssupporting

confidence: 60%

Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.

Stanbridge¹,

Rosen²,

Sussman³

1982

Proc. Natl. Acad. Sci. U.S.A.

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The expression of HeLa parent phenotype protein markers, the alpha subunit of human chorionic gonadotropin and placental alkaline phosphatase isoenzymes, has been evaluated in paired tumorigenic and nontumorigenic HeLa-fibroblast human cell hybrids. Both of these proteins have been used clinically as markers of malignancy. The results showed that both are expressed in the hybrids. Expression of the gonadotropin subunit in the hybrids is specifically correlated with tumorigenicity; the placental alkaline phosphatase isoenzyme showed no such correlation and was expressed in both tumorigenic and nontumorigenic hybrids.

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“…invariant marker of malignancy in this experimental system. A similar conclusion has been reached from the study of a variety of different human tumor-derived cell lines (34).…”

Section: Methodssupporting

confidence: 60%

Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.

Stanbridge¹,

Rosen²,

Sussman³

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…In this study we present a detailed karyotypic analysis of four cell lines derived from human bladder urothelial carcinomas, in which chromosome markers which are consistently present have been identified. Previous studies on these four cell lines have included plating efficiency (Marshall et al, 1977), distribution of extramembraneous alkaline phosphatase and placental-type isoenzyme (Benham et al, 1977;Wilson et al, 1977), fibrinolytic activity (Pearlstein et al, 1976;Marshall et al, 1977), loss of density-dependent inhibition of growth and absence of LETS protein (Marshall et al, 1977). Although the chromosome numbers of some of the cell lines have been described previously [RT4 (Rigby and Franks, 1970), T24 (Malkovsky and Bubenfk, 1977;Kato et al, 1978) and EJ (Kato et al, 1978)], detailed banded karyotypes are presented here for the first time.…”

mentioning

confidence: 99%

Chromosome pattern, growth in agar and tumorigenicity in nude mice of four human bladder carcinoma cell lines

Hastings

Franks

1981

Intl Journal of Cancer

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Four human bladder carcinoma cell lines have been characterized by G-banding, and the chromosomal patterns correlated to growth in agar and tumorigenicity in nude mice. Each cell line was shown to be chromosomally unique and although numerical and structural anomalies were present, none were common to all four cell lines. However, one or more copies of a structurally altered chromosome 8 were present in all four cell lines and may be associated with tumorigenicity in nude mice. A combination of three marker chromosomes was found in the more anaplastic cell lines, but not in the two well-differentiated tumour cell lines. Growth in agar may be associated with the presence of the three marker chromosomes but was not correlated with tumorigenicity in nude mice.

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“…There was uniform distribution in young animals but enhanced periportal activity in the old animals. The reason for this is obscure as alkaline phosphatase is a heterogeneous group of plasma membrane-associated enzymes with unknown function, but it has been shown to be an enzyme particularly susceptible to change with age and in tissue culture (Wilson, 1973a, Wilson et al, 1977.…”

Section: Discussionmentioning

confidence: 99%

Differential Enzyme Distribution in Lobules of Livers from Young and Old Mice and Rats

Wilson¹

1978

Gerontology

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In the livers from young (3–6 month) and old (30 month) C57/BL mice and BN/Bi rats light microscope histochemistry has shown that enzyme activity is not always distributed evenly throughout the lobule. The mitochondrial enzyme succinic dehydro-genase, the plasma membrane enzyme 5’-nucleotidase and the endoplasmic reticulum enzyme glucose-6-phosphatase showed heavier reaction product in the periportal regions of the lobule compared with the centrilobular regions. Alkaline phosphatase showed an altered distribution pattern with age: in young livers this was uniform throughout the lobule while in old livers there was enhanced periportal activity. Electron microscope cytochemistry showed that this was due to increased numbers of bile canaliculi in this region containing reaction product and to the additional presence of reaction product associated with the microvilli lining the space of Disse.

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Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: Not an invariable marker for neoplastic transformation

Cited by 14 publications

References 12 publications

Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.

Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.

Chromosome pattern, growth in agar and tumorigenicity in nude mice of four human bladder carcinoma cell lines

Differential Enzyme Distribution in Lobules of Livers from Young and Old Mice and Rats

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