Alkaline phosphatase in mitochondria

Wilson, Patricia D.; Franks, L. M.; Cottell, David C.; Benham, Frances

doi:10.1016/0309-1651(77)90014-5

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Therefore, mitochondria contain the enzymes needed to catalyze these physiological processes, as well as enzymes that catalyze protein synthesis. It is reported that the activity of ALP in mitochondria is relatively high . ALP can catalyze the removal of 5′-phosphate groups from DNA, RNA, ribonucleoside triphosphate, and deoxyribonucleoside triphosphate and plays important roles in the synthesis of DNA and RNA in mitochondria .…”

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confidence: 99%

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Ratiometric Fluorescent Strategy for Localizing Alkaline Phosphatase Activity in Mitochondria Based on the ESIPT Process

Zhang

et al. 2019

Anal. Chem.

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Fluorescent probes are powerful tools for detecting and mapping the species of interest in vitro and in vivo. Although the probes always show high selectivity and sensitivity, they are usually affected by some factors, such as detecting conditions and the probe concentrations. Ratiometric fluorescent strategies, possessing advantage of low background noise, would solve the problem effectively and lead to a higher sensing performance. Thus, an ESIPT-based ratiometric probe (HBTP-mito) was developed on the basis of a phosphorylated 2-(2′-hydroxyphenyl)-benzothiazole derivative for the determination of ALP activity. HBTP-mito is water soluble and emits green fluorescence in TBS buffer due to the blockage of ESIPT. Upon the introduction of ALP, the phosphate ester of HBTP-mito was hydrolyzed and the ESIPT process was restored. Accordingly, the fluorescence at 514 nm decreases, while emission at 650 nm shows a “turn-on” response. The ratio of intensity (I 514nm/I 650nm) decreases linearly with ALP activity increasing from 0 to 60 mU/mL, obtained an LOD of 0.072 mU/mL. The favorable performance of the probe enables its application not only in the detection of ALP activity in biological samples, but also in the localization of the ALP levels in living cells and in vivo.

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confidence: 99%

“…It is reported that the activity of ALP in mitochondria is relatively high. 37 ALP can catalyze the removal of 5′-phosphate groups from DNA, RNA, ribonucleoside triphosphate, and deoxyribonucleoside triphosphate and plays important roles in the synthesis of DNA and RNA in mitochondria. 38 Therefore, it is necessary to detect ALP activity in mitochondria.…”

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confidence: 99%

Ratiometric Fluorescent Strategy for Localizing Alkaline Phosphatase Activity in Mitochondria Based on the ESIPT Process

Zhang

et al. 2019

Anal. Chem.

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“…WILSON and FRANKs [20] reported that ALPase was present in the cell membrane and in mitochondria which showed that they have the same isoenzyme pattern. But GRANDSTROM et al [21] ALPase reaction existed in the Golgi apparatus.…”

Section: Discussionmentioning

confidence: 98%

Ultrastructural localization of alkaline phosphatase in fish enamel organ (Oplegnathus fasciatus).

Inage¹,

Kasamo²,

Yoshida³

et al. 1984

The Journal of Nihon University School of Dentistry

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“…Expression of this enzyme is increased when the bile ducts become blocked. SGOT and SGPT are both enzymes that are mainly found in mitochondria of the liver (28). If liver damage is present, the enzymes are released into the bloodstream following liver cell death (29).…”

Section: Discussionmentioning

confidence: 99%

Effect of glabridin on collagen deposition in liver and amelioration of hepatocyte destruction in diabetes rats

et al. 2019

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Abnormalities in insulin hormone levels leads to a hyperglycemic condition of diabetic mellitus. Hyperglycemia seriously induces organ and system destructions. The excessive accumulation of collagen fiber deposits occurs in inflammatory and reorganization processes of chronic liver diseases in type I insulin-dependent diabetes. Regarding the research objective, glabridin (GLB), an active compound of licorice, was used as a daily supplement (40 mg/kg) in order to decrease hepatocyte destruction and collagen deposition in liver tissue of diabetic animals induced by streptozotocin. A total of 40 were randomly allocated to five groups (each, n=10), control, control treated with GLB (GLB), diabetic rats (DM) injected with single dose of streptozotocin (60 mg/kg) to induce a diabetic condition, diabetic rats receiving GLB (DM+GLB; 40 mg/kg) and diabetic rats treated with glibenclamide (DM+GL; 4 mg/kg). Characteristic histopathological changes in liver cells and tissues of rats were determined by Masson's trichrome staining and transmission electron microscopy (TEM). Western blotting was used to detect the expression of the key markers, collagen type I and fibronectin proteins. The histological investigation of liver tissue of the DM group revealed that the collagen fiber deposition was increased in the periportal, pericentral and perisinusoidal spaces compared with controls. Hepatocytes appeared as small and fragmented cells in TEM examination. Collagenization of the perisinusoidal space was recently demonstrated to represent a new aspect of the microvascular abnormalities and liver fibrosis. Healthy hepatocytes with round nucleus were observed following supplementation of glabridin. In addition, collagen fiber deposition was reduced in the area adjacent to the perisinusoidal space. The expression of collagen type I and fibronectin decreased strongly following glabridin supplementation in DM+GLB rats compared with DM rats, indicating that the hepatic tissue reorganization regained its normal morphology. These findings suggest that it may be beneficial to examine the role of glabridin as a therapeutic agent in diabetes treatment in future research.

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Alkaline phosphatase in mitochondria

Cited by 7 publications

References 12 publications

Ratiometric Fluorescent Strategy for Localizing Alkaline Phosphatase Activity in Mitochondria Based on the ESIPT Process

Ratiometric Fluorescent Strategy for Localizing Alkaline Phosphatase Activity in Mitochondria Based on the ESIPT Process

Ultrastructural localization of alkaline phosphatase in fish enamel organ (Oplegnathus fasciatus).

Effect of glabridin on collagen deposition in liver and amelioration of hepatocyte destruction in diabetes rats

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