Ultrastructural localization of alkaline phosphatase in fish enamel organ (Oplegnathus fasciatus).

Fine structural and cytochemical observations of dental epithelial cells during the enameloid formation stages in red stingrays Dasyatis akajei

“…ACP, ACPase; ALP, ALPase; CaATP, Ca-ATPase; CP, capillary; CR, crystal; D, demineralized; E, enameloid; G, glycogen particles; IDE, inner dental epithelium; ODE, outer dental epithelium; UD, undemineralized. and the early stage of enameloid maturation (Inage et al, 1984;Shimoda, 1989;Sasagawa, 1998). In mammals, it was reported that ALPase activity is localized at the stratum intermedium and proximal cell membrane of ameloblasts in the formation stage; in the maturation stage the activity was seen at the lateral cell membrane and distal ruffled border in RA, at the lateral cell membrane in smoothended ameloblasts (SA), and at the papillary layer (Kurahashi and Yoshiki, 1972;Deporter and Ten Cate, 1976;Orams and Snibson, 1982;Takano and Ozawa, 1980).…”

“…There are several reports on enzyme histochemistry during tooth development in fish, mainly teleosts (Yamada and Ozawa, 1978;Inage et al, 1984;Shi-moda, 1989;Sasagawa, 1993Sasagawa, , 1998. Two reports (Kerr, 1955;Sasagawa, 1997) examined elasmobranchs, but employed only light microscopy.…”

Activity of alkaline and acid phosphatases in dental epithelial cells and enameloid during odontogenesis in two teleost fish, Oreochromis niloticus and Tilapia buttikoferi

“…T he enameloid in teleosts is produced by both the odon toblasts and the dental epithelia l cell s, while matured enameloid is a high ly minera lized tiss ue covering tooth crowns as well as the ectod ermal enamel in mammals. The formation of the enameloid i a topic of interest to researchers who are stud ying comparative odontology and the basic mechanisms of minera li za tion durin g tooth development, and is expected to provide intriguing evidence concerning the hi sto ry of odontogenesis, such as the role of the dental epithelial cell s. A number of ultrastructural studie have been performed during the past three decades but a few studies examined enzyme hi stochemi stry during odontoge nesis in teleosts (1)(2)(3)(4), and only parts of the odontogenesis were in vestigated . An exception is the stud y of INAGE et a!.…”

“…An exception is the stud y of INAGE et a!. (2), in w hich the localiza tion of alkaline phosphatase (ALPa e) activity in the dental epitheli a l cell s during tooth de elopmen t was observed. However, th ey failed to find the acid phosphatase ( ACPase) activity, and their materials, Oplegnathus fasciatus, Oplegnathidae, do not possess isolated teeth but unique teeth constituting tooth plates in the jaws.…”