Fine structural and cytochemical mapping of enamel organ during the enameloid formation stages in gars, Lepisosteus oculatus, Actinopterygii

Sasagawa, Ichiro; Ishiyama, Mikio

doi:10.1016/j.archoralbio.2004.07.013

Cited by 19 publications

(20 citation statements)

References 42 publications

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“…In Lepisosteus, amelogenin-like proteins have also been detected in the granules of the ameloblasts facing the enameloid layer during the enameloid mineralization that occurs prior to enameloid maturation and collar enamel formation but they have not been found in or on the enameloid matrix (Ishiyama et al 2001). The appearance of amelogenin-like proteins in the ameloblasts occurs slightly earlier than the formation of collar enamel in Lepisosteus (Sasagawa and Ishiyama 2005a). The ameloblasts are suggested to have the ability to produce amelogenin-like proteins throughout odontogenesis but only release them during the collar enamel formation stage (Ishiyama et al 1994(Ishiyama et al , 2001.…”

Section: Enamel Matrix Covers the Cap Enameloid As A Terminal Productmentioning

confidence: 94%

Fine structural and immunohistochemical detection of collar enamel in the teeth of Polypterus senegalus, an actinopterygian fish

et al. 2012

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This is the first detailed report about the collar enamel of the teeth of Polypterus senegalus. We have examined the fine structure of the collar enamel and enamel organ of Polypterus during amelogenesis by light and transmission electron microscopy. An immunohistochemical analysis with an antibody against bovine amelogenin, an antiserum against porcine amelogenin and region-specific antibodies or antiserum against the C-terminus, middle region and N-terminus of porcine amelogenin has also been performed to examine the collar enamel matrix present in these teeth. Their ameloblasts contain fully developed Golgi apparatus, rough endoplasmic reticulum and secretory granules. During collar enamel formation, an amorphous fine enamel matrix containing no collagen fibrils is found between the dentin and ameloblast layers. In non-demineralized sections, the collar enamel (500 nm to 1 μm thick) is distinguishable from dentin, because of its higher density and differences in the arrangement of its crystals. The fine structural features of collar enamel in Polypterus are similar to those of tooth enamel in Lepisosteus (gars), coelacanths, lungfish and amphibians. The enamel matrix shows intense immunoreactivity to the antibody and antiserum against mammalian amelogenins and to the middleregion- and C-terminal-specific anti-amelogenin antibodies. These findings suggest that the proteins in the enamel of Polypterus contain domains that closely resemble those of bovine and porcine amelogenins. The enamel matrix, which exhibits positive immunoreactivity to mammalian amelogenins, extends to the cap enameloid surface, implying that amelogenin-like proteins are secreted by ameloblasts as a thin matrix layer that covers the cap enameloid after enameloid maturation.

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Section: Enamel Matrix Covers the Cap Enameloid As A Terminal Productmentioning

confidence: 94%

Fine structural and immunohistochemical detection of collar enamel in the teeth of Polypterus senegalus, an actinopterygian fish

et al. 2012

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“…Stratum intermedium was not recognized in the enamel organ facing the collar enamel, although these cells were marked in the cap enameloid area [7,46]. Stellate reticulum cells in the collar enamel area exhibit Ca-ATPase activity, although these cells hardly show marked activities of ALPase, ACPase, NPPase (Na/K-ATPase) (Sasagawa et al, unpublished data).…”

Section: Comparison With the Formation Of Cap Enameloid In Lepisosteusmentioning

confidence: 94%

“…The IDE cells having the ruffled border resemble the ruffle-ended ameloblasts (RA) found in mammals, suggesting their active function of material transportation during enameloid mineralization [7,8]. In Lepisosteus, the IDE cells possessing ruffled borders are present at the enameloid cap, but not in the collar enamel area.…”

Section: Comparison With the Formation Of Cap Enameloid In Lepisosteusmentioning

confidence: 98%

“…During early cap enameloid formation, procollagen granules containing cross-striations at intervals of approximately 60 nm has been found in the inner dental epithelial (IDE) cells (ameloblasts) [7]. However, there were no procollagen granules in the ameloblasts facing the collar enamel.…”

Section: Comparison With the Formation Of Cap Enameloid In Lepisosteusmentioning

confidence: 99%

“…There have seen only two studies concerning the collar enamel in Lepisosteus [1,2], in addition to several studies on their general tooth development [5][6][7][8]. The previous studies focused on the secretory stage.…”

Section: Introductionmentioning

confidence: 96%

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Fine structure and development of the collar enamel in gars, Lepisosteus oculatus, Actinopterygii

Sasagawa

Ishiyama

Yokosuka

et al. 2008

Front. Mater. Sci. China

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The fine structure of collar enamel and the cells constituting the enamel organ during amelogenesis in Lepisosteus oculatus was observed by light, scanning electron and transmission electron microscopy. In the enamel, slender crystals were arranged perpendicular to the surface and the stripes that were parallel to the surface were observed, suggesting that the enamel in Lepisosteus shares common morphological features with that in sarcopterygian fish and amphibians. Ameloblasts containing developed Golgi apparatus, rough endoplasmic reticulum (rER) and secretory granules were found in the secretory stage. In the maturation stage, a ruffled border was not seen at the distal end of the ameloblasts, while many mitochondria and lysosome-like granules were obvious in the distal cytoplasm. The enamel organ consisted of the outer dental epithelial cells, stratum reticulum cells and ameloblasts, but there was no stratum intermedium. It is likely that the ameloblasts have less absorptive function in comparison with the inner dental epithelial cells facing cap enameloid.

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Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium‐binding phosphoprotein family

2005

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Three principal mineralized tissues are present in teeth; a highly mineralized surface layer (enamel or enameloid), body dentin, and basal bone. Similar tissues have been identified in the dermal skeleton of Paleozoic jawless vertebrates, suggesting their ancient origin. These dental tissues form on protein matrix and their mineralization is controlled by distinctive proteins. We have shown that many secretory calcium-binding phosphoproteins (SCPPs) are involved in tetrapod tissue mineralization. These SCPPs all originated from the common ancestral gene SPARCL1 (secreted protein, acidic, cysteine-rich like 1) that initially arose from SPARC. The SCPP family also includes a bird eggshell matrix protein, mammalian milk casein, and salivary proteins. The eggshell SCPP plays crucial roles in rigid eggshell production, milk SCPPs in efficient lactation and in the evolution of complex dentition, and salivary SCPPs in maintaining tooth integrity. A comparative analysis of the mammalian, avian, and amphibian genomes revealed a tandem duplication history of the SCPP genes in tetrapods. Although these tetrapod SCPP genes are fewer in teleost genomes, independent parallel duplication has created distinct SCPP genes in this lineage. These teleost SCPPs are also used for enameloid and dentin mineralization, implying essential roles of SCPPs for dental tissue mineralization in osteichthyans. However, the SCPPs used for tetrapod enamel and teleost enameloid, as well as tetrapod dentin and teleost dentin, are all different. Thus, the evolution of vertebrate mineralized tissues seems to be explained by phenogenetic drift: while mineralized tissues are retained during vertebrate evolution, the underlying genetic basis has extensively drifted.

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Fine structural and cytochemical mapping of enamel organ during the enameloid formation stages in gars, Lepisosteus oculatus, Actinopterygii

Cited by 19 publications

References 42 publications

Fine structural and immunohistochemical detection of collar enamel in the teeth of Polypterus senegalus, an actinopterygian fish

Fine structural and immunohistochemical detection of collar enamel in the teeth of Polypterus senegalus, an actinopterygian fish

Fine structure and development of the collar enamel in gars, Lepisosteus oculatus, Actinopterygii

Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium‐binding phosphoprotein family

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