Characterisation of phenolic antioxidants in aqueous extract of Orthosiphon grandiflorus tea by LC–ESI-MS/MS coupled to DPPH assay

Nuengchamnong, Nitra; Krittasilp, Kamrai; Ingkaninan, Kornkanok

doi:10.1016/j.foodchem.2011.01.085

Cited by 53 publications

(31 citation statements)

References 30 publications

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“…MS data for RA showed a prominent [M-H] -product ion at m/z 358.8, which was assigned to the molecular ion (MW 360). The fragmentation pattern with prominent peaks at m/z 197.0, 179.2, 160.9, 134.9 in the negative ion spectrum was consistent with that reported in the literature (Nuengchamong et al 2011). The fragmentations at m/z 179.2 and m/z 197.0 indicate the presence of a caffeic acid moiety and 2-hydroxy derivative of hydrocaffeic acid, respectively.…”

Section: Resultssupporting

confidence: 88%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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Hairy roots of Dracocephalum moldavica L. were induced using Agrobacterium rhizogenes strain A4. Transformed roots were obtained from shoot explants with low transformation frequency of up to 3 %. The effects of different liquid media: Murashige and Skoog (MS), Gamborg et al. (B5) and Woody Plant (WP) with full-and half-strength ( MS, B5, WP), on biomass accumulation and rosmarinic acid (RA) content were investigated. The hairy roots were cultured in photoperiod (16 h light/8 h dark) and darkness. Biomass of D. moldavica hairy roots was the highest (7.23 g flask -1 of fresh weight and 0.89 g flask -1 of dry weight) in the cultures grown in WP medium under periodic light. Ultra performance liquid chromatography analysis revealed the highest RA content (78 mg g -1 dry wt) in roots cultured in B5 medium under photoperiod conditions. It was about tenfold higher compared to roots of field-grown mother plants. Antioxidant activities and total phenolic contents of methanolic extracts of D. moldavica hairy roots cultured in B5 and WP media under photoperiod and darkness and roots of field grown plants were compared. All extracts were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and phosphomolybdenum reduction assays. Total phenolic contents were estimated by the FolinCiocalteu method. The methanolic extract of D. moldavica hairy roots grown in B5 medium under photoperiod possessed the strongest effects on reducing Mo and DPPH radical scavenging. The activities were significantly higher (p B 0.05) than those of methanolic extract of roots of intact plants grown in the field. The most active methanolic extract of hairy roots was characterized by the highest level of rosmarinic acid and total content of phenolic compounds.

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Section: Resultssupporting

confidence: 88%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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“…The petroleum ether extract was found to contain the highest (p b 0.05) amounts of carnosic acid (531.3 mg/g) and carnosol (389.9 mg/g) among all extracts, and a very low amount of apigenin (1.3 mg/g), but to contain none of the other phenolic acids and flavonoids that are relatively more polar. The methanol extract was found to contain the highest amount of caffeic acid (255.6 mg/g, p b 0.05) and significant amount of rosmarinic acid (109.9 mg/g), both of which were reported to act as powerful antioxidants (Nuengchamnong, Krittasilp, & Ingkaninan, 2011). These two phenolic acids were also detected to exist in relatively high amounts in acetone extract compared to other phenolics (Table 1).…”

Section: Phenolic Compoundsmentioning

confidence: 95%

Phenolic compounds profile and antioxidant activity of Dorystoechas hastata L. Boiss et Heldr

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Akgonen

Ovat

et al. 2011

Food Research International

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“…Twenty compounds could be detected and identified, mostly corresponding to caffeic acid derivatives. Peak characteristics and tentative identifications based on their UV and mass spectra by comparing with the available literature [16][17][18][19][20][21][22] are presented in were tentatively identified as hydroxycinnamoyl tartaric esters, namely caftaric (caffeoyltartaric acid), fertaric (feruloyltartaric acid) and cichoric (dicaffeoyltartaric acid) acids, respectively, based on their mass spectra. 20 Compound…”

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confidence: 99%

Melissa officinalis L. decoctions as functional beverages: a bioactive approach and chemical characterization

et al. 2015

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Lemon balm (Melissa officinalis L.) is a member of the Lamiaceae family with a long story of human consumption. It has been consumed for decades, directly in food and as a decoction or an infusion for its medicinal purposes. In this manuscript, a detailed chemical characterization of the decoction of this plant is described, encompassing antimicrobial, antioxidant and antitumor activities. Rosmarinic acid and lithospermic acid A were the most abundant phenolic compounds. Quinic acid, fructose, glucose and γ-tocopherol were the most abundant within their groups of molecules. M. officinalis decoctions were active against a wide range of microorganisms, Pseudomonas aeruginosa and Salmonella typhimurium, and Penicillium funiculosum being the most sensitive bacteria and fungi, respectively. The growth inhibition of different human tumor cell lines (mainly MCF-7 and HepG2) was also observed, as also high free radical scavenging activity and reducing power. This manuscript highlights some beneficial effects of these functional beverages.

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Characterisation of phenolic antioxidants in aqueous extract of Orthosiphon grandiflorus tea by LC–ESI-MS/MS coupled to DPPH assay

Cited by 53 publications

References 30 publications

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Phenolic compounds profile and antioxidant activity of Dorystoechas hastata L. Boiss et Heldr

Melissa officinalis L. decoctions as functional beverages: a bioactive approach and chemical characterization

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