Characterisation of bacterial communities associated with early stages of intensively reared cod (Gadus morhua) using Denaturing Gradient Gel Electrophoresis (DGGE)

Brunvold, Laila; Sandaa, Ruth‐Anne; Mikkelsen, Helene; Welde, Eirik; Bleie, Hogne; Bergh, Øivind

doi:10.1016/j.aquaculture.2007.08.053

Cited by 66 publications

(47 citation statements)

References 39 publications

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“…During first feeding, yolk-sac larvae in general are subject to a massive inflow of bacteria from the live feed organisms Brachionus plicatilis and Artemia spp., resulting in a shift in intestinal bacterial flora (Brunvold et al 2007), and suspected pathogens such as V. anguillarum may accumulate in the live feed (Korsnes et al 2006). The mucosal surfaces of fish serve as a substrate for bacterial adhesion (Spangaard et al 2000) and thereby a potential portal of entry.…”

Section: Introductionmentioning

confidence: 99%

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Sandlund

Bergh²

2008

Dis. Aquat. Org.

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In intensive aquaculture systems, high concentrations of nutrients and high densities of fish larvae provide favorable conditions for opportunistic pathogenic bacteria to flourish. We screened potentially pathogenic bacterial strains isolated from moribund Atlantic cod Gadus morhua larvae, pollack Pollachius pollachius, coalfish Pollachius virens, Atlantic halibut Hippoglossus hippoglossus, rotifers, algae and water samples from different hatcheries. Three identical challenge experiments tested a total of 53 strains. A multidish system was used: cod eggs were placed in single wells, together with 2 ml of sterile seawater, and exposed to the bacterial cultures. Final bacterial concentrations in the wells were 10 6 and 10 4 CFU ml -1. Eggs and larvae not exposed to bacteria were used as unchallenged controls. Challenged controls were exposed to Vibrio anguillarum strain 610. Eggs were challenged approximately 48 h prior to hatching and mortality was recorded daily throughout the yolk-sac period. In spite of the high challenge dose of 10 6 CFU ml -1, only 5 bacterial strains tested caused higher mortality than the unchallenged controls. Four of these strains were identified by 16S rDNA and gyrase B gene (GyrB) sequencing as resembling V. anguillarum and 1 strain resembled Carnobacterium sp. Most of the larvae exposed to these strains died within 10 d of challenge. Serotyping of the strains resembling V. anguillarum gave inconclusive results. This indicates differences in serology compared to the serotypes O1, O2 and O3, associated with disease. Three bacterial strains seemed to have a slower infection rate, indicating a longer incubation period. The remaining 45 strains did not seem to have a negative effect on larval survival, suggesting that these are not primary pathogens.

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Section: Introductionmentioning

confidence: 99%

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Sandlund

Bergh²

2008

Dis. Aquat. Org.

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“…The antagonism of Phaeobacter 27-4 and the closely related Phaeobacter inhibens is due mainly to the sulfur-containing tropolone derivative tropodithietic acid (TDA) (2,5), which is also produced by other Phaeobacter strains and Ruegeria mobilis (28). Phaeobacter and Ruegeria strains or their DNA has been commonly found in marine larva-rearing sites (6,17,28).…”

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confidence: 99%

Inactivation of Vibrio anguillarum by Attached and Planktonic Roseobacter Cells

D’Alvise

Melchiorsen

Porsby

et al. 2010

Appl Environ Microbiol

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The purpose of the present study was to investigate the inhibition of Vibrio by Roseobacter in a combined liquid-surface system. Exposure of Vibrio anguillarum to surface-attached roseobacters (107 CFU/cm2) resulted in significant reduction or complete killing of the pathogen inoculated at 102 to 104 CFU/ml. The effect was likely associated with the production of tropodithietic acid (TDA), as a TDA-negative mutant did not affect survival or growth of V. anguillarum.

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“…Vibrio sp. was found as the dominating genus associated with moribund cod larvae by Brunvold et al (2007). Although, the majority of sequences in the present study could not be attributed to recognised species of the genus Vibrio, some of the sequences associated with the flowthrough systems showed 99% homology to Vibrio splendidus and Vibrio sinaloensis.…”

Section: Accepted M Manuscriptmentioning

confidence: 64%

“…Studies have reported the limitations of 16S rDNA-based analysis, due to low variation in the 16S rRNA gene that makes it difficult to discriminate between bacterial species (Case et al, 2007). Polymorphism between repeated 16S rRNA genes within genomes of Vibrio are known to exist (Moreno et al, 2002), and the use of DGGE may further add to this problem in obtaining reliable sequences for phylogenetic analysis (Sekiguchi et al, 2001, Brunvold et al, 2007.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Water quality and microbial community structure in juvenile Atlantic cod (Gadus morhua L.) cultures

et al. 2011

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Havforskningsinstituttets institusjonelle arkiv Brage IMR - Institutional repository of the Institute of Marine Research b r a g e i m rDette er forfatters siste versjon av den fagfellevurderte artikkelen, vanligvis omtalt som postprint. I Brage IMR er denne artikkelen ikke publisert med forlagets layout fordi forlaget ikke tillater dette. Du finner lenke til forlagets versjon i Brage-posten. Det anbefales at referanser til artikkelen hentes fra forlagets side. Ved lenking til artikkelen skal det lenkes til post i Brage IMR, ikke direkte til pdf-fil.This is the author's last version of the article after peer review and is not the publisher's version, usually referred to as postprint. You will find a link to the publisher's version in Brage IMR. It is recommended that you obtain the references from the publisher's site.Linking to the article should be to the Brage-record, not This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT AbstractThe effect of water treatment and flow rate on young Atlantic cod juveniles was investigated in a 36-days experiment. Four different flow rates (10, 20, 40, and 70 times the effective tank volume per day) were set up in triplicate tanks within each of three rigs with recirculated, UV-radiated, and untreated water, respectively. Each of the 36 tanks was stocked with 200 weaned cod juveniles at a mean weight of 0.048g. Fish mortality was recorded daily in all tanks, and growth (wet weight) was determined at the end of the experiment. The microflora in the rearing water was investigated by means of PCR-DGGE and flow cytometry. Observed mortality was significantly higher at low flow rates while otherwise unexplained mortality (presumed to be due to cannibalism) was lowest in the recirculation system. No correlation was found between survival and growth. Growth was significantly affected by both water exchange rate and treatment, as the juveniles from high flow rates and the UV-treatment showed elevated growth rates. Both growth and survival scaled in accordance with metabolic Runar Thyrhaug passed away January 7, 2011. The authors wish to dedicate this paper to his memory. * Corresponding author. Tel.: +47 56182262; fax: +47 56182222. E-mail address: Terje.van.der.Meeren@imr.no Deceased A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 factors like oxygen saturation and unionized ammonia. Bacterial concentrations increased in all tanks and treatment from the beginning of the experiment to the end. The UV-treated and untreated water started at typical seawater concentrations (0.5-1 x10 6 mL -1 ) and increased five t...

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Characterisation of bacterial communities associated with early stages of intensively reared cod (Gadus morhua) using Denaturing Gradient Gel Electrophoresis (DGGE)

Cited by 66 publications

References 39 publications

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Screening and characterisation of potentially pathogenic bacteria associated with Atlantic codGadus morhualarvae: bath challenge trials using a multidish system

Inactivation of Vibrio anguillarum by Attached and Planktonic Roseobacter Cells

Water quality and microbial community structure in juvenile Atlantic cod (Gadus morhua L.) cultures

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