Characterisation of acid-soluble collagen from skin of silver carp (Hypophthalmichthys molitrix)

Zhang, Junjie; Duan, Rui; Tian, Yuanyong; Konno, Kunihiko

doi:10.1016/j.foodchem.2009.02.053

Cited by 79 publications

(46 citation statements)

References 17 publications

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“…The imino acid contents of collagens derived from the skin byproducts of paddlefish and globefish were 194.1-197.9 and 186.4-189.7 residues/1000 residues, respectively, which were analogous to that of unicorn leatherjacket (187-190 residues/1000 residues) (Ahmad et al, 2010), whilst higher than those of cold water fish such as cod and deep-sea redfish (154 and 165 residues/1000 residues) (Wang et al, 2008;Zhang et al, 2009a). The less content of imino acid could lead to inferior thermal stability and lower melting point (Singh et al, 2011).…”

Section: Amino Acid Compositionmentioning

confidence: 91%

Physicochemical characteristics and fibril-forming properties of collagen from paddlefish (Polyodon spathula) and globefish (Fugu flavidus) skin byproducts

2017

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Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the skin byproducts of paddlefish (ASC-P and PSC-P) and globefish (ASC-G and PSC-G) were purified and characterized. The imino acid contents of ASC-P, PSC-P, 197.9, 186.4 and 189.7 residues/1000 residues, respectively. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) confirmed that all four samples composed of two different α1-and α2-chains with integrated triple-helical structure. Denaturation temperatures of ASC-P, PSC-P, ASC-G and PSC-G were 29.6, 28.2, 27.4 and 26.9 °C, respectively. Based on Transmission electron microscopy (TEM) observation, all four samples could assemble into fibrils in vitro with D-periodicity. However, the fibril-forming rate of ASC-P and PSC-P was more rapid than that of ASC-G and PSC-G. Scanning electron microscopy (SEM) analysis confirmed well-defined fibril morphologies,the diameter of fibrils from ASC-P and PSC-P was thicker than those of ASC-G and PSC-G after 24 h incubation. These results indicated that paddlefish and globefish skin collagens could be alternatives to terrestrial collagens for applications in food-packaging, nutraceutical and pharmaceutical industries.Keywords: paddlefish; globefish; collagen; physicochemical property; fibril-forming property; fibril morphology.Practical Application: Utilization of paddlefish and globefish skin byproducts by extracting collagen with good physicochemical and fibril-forming properties.

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Section: Amino Acid Compositionmentioning

confidence: 91%

Physicochemical characteristics and fibril-forming properties of collagen from paddlefish (Polyodon spathula) and globefish (Fugu flavidus) skin byproducts

2017

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“…The methods described by Zhang, Duan, et al (2009) and Liu, Zhong, et al (2010), with some slight modifications, were used to isolate and purify tilapia collagen.…”

Section: Isolation Of Acid-soluble Tilapia Collagenmentioning

confidence: 99%

“…The skin and meat of tilapia are known to contain a large quantity of collagen. Nowadays, tilapia is also a major source of collagen for industrial uses (Zhang, Duan, Tian, & Konno, 2009;Zhang, Liu, et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

IgE reactivity to type I collagen and its subunits from tilapia (Tilapia zillii)

Pan

Cao

et al. 2012

Food Chemistry

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“…They modify the functional properties such as emulsification, fat-binding, water-binding, foaming properties, gel strength, whipping properties (Sawant and Nagendran, 2014), as well as bioactive peptides synthesis (Zhong et al, 2011). Thus, proteases are using on a large scale in the food industry, where it is estimated to represent about 40-65% of the total commercial enzyme preparations in the global market (Zhang et al, 2009). In the industrial view, proteases can use as pharmaceutical agents (e.g.…”

mentioning

confidence: 99%

“…Huge amounts of silver carp by products (heads, skin, bone, scales and viscera) are produced during the fish processing. This became as resources of hazard environmental pollution resources wastes (Zhang et al, 2009). Viscera is one of the most important by products delivered digestive enzymes, especially proteases that have high activity over a wide range of pH and temperature conditions .…”

mentioning

confidence: 99%

Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish

Atta

El-Hamed

Keshk

2017

AJAS

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Proteases from viscera of Silver carp (Hypophthalmichthys molitrix) fish have been extracted and characterized. The alkaline proteases show optimum activity in 0.2M Tris-HCl buffer at pH 8.5 and 45°C using soluble milk casein as substrate. The crude alkaline protease lost about 35% or 51% of its specific activity if it was heated at 50C or kept at pH 9 for 60 min., respectively. Purification of proteases purified by ammonium sulfate precipitation, gel-filtration using Sephadex G 50 column and ultrafiltration via polyamide membrane (30 KDa) led to increase its specific activity up to 20, 24 and 99 fold, respectively.

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Characterisation of acid-soluble collagen from skin of silver carp (Hypophthalmichthys molitrix)

Cited by 79 publications

References 17 publications

Physicochemical characteristics and fibril-forming properties of collagen from paddlefish (Polyodon spathula) and globefish (Fugu flavidus) skin byproducts

Physicochemical characteristics and fibril-forming properties of collagen from paddlefish (Polyodon spathula) and globefish (Fugu flavidus) skin byproducts

IgE reactivity to type I collagen and its subunits from tilapia (Tilapia zillii)

Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish

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