Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish

Atta, Mohamed; El-Hamed, Shimaa El-Said Abd; Keshk, Samia A.

doi:10.21608/ajas.2017.7572

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The results presented in Table 2 revealed the highest EA at pH of 8 and temperature of 50°C. Previous studies also indicated that enzymes extracted from marine and fisheries substance with neutral buffers tend to have optimum EA at alkaline condition (pH of 8-9) and temperature range 45-60°C [3,18,19]. Vannabun et al (2014) reported higher EA of alkaline protease enzyme extracted from giant catfish viscera compared to acid protease [20].…”

Section: Enzyme Activity Of Crude Enzymementioning

confidence: 91%

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Tamaya,

Suwanto,

Hernawan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The goal of this study was to investigate the impact of UV-C irradiation on the activity of crude enzymes derived from African Catfish intestines as well the optimum conditions for subsequent autolysis processes. Three key variables were assessed: UV-C exposure time, pH and temperature. The experiments involved the homogenization of intestines in cold distilled water followed by subjecting the mixture into UV-C treatment for exposure times of 0, 10, 20 and 30 minutes. After centrifugation process, the collected supernatant was utilized as the crude enzyme. At various level of temperature (40, 50, 60°C) and pH (6, 7, 8) the activity of crude enzyme was assayed with casein as substrate. The findings revealed the increasing of EA as the UV-C exposure time decreased and pH increased, while the highest EA value was observed at temperature of 50°C. Consequently, the optimal conditions were identified as follows: 0 minutes of UV-C exposure time, pH of 8, and temperature of 50°C. Furthermore, UV-Vis absorption, FTIR, and fluorescence spectroscopy of the crude enzyme after UV-C irradiation induction was studied to investigate the its conformational changes. These additional analyses provided valuable insights into the structural alterations of the crude enzyme caused by the UV-C treatment.

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Section: Enzyme Activity Of Crude Enzymementioning

confidence: 91%

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Tamaya,

Suwanto,

Hernawan

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Proteases are the most widely used group of enzymes in industrial bioprocesses, which is why they represent about 40%-65% of the total commercial enzyme preparations (Atta et al, 2017). In this sense, fish viscera are one of the most important wastes because they contain digestive enzymes, especially aspartic proteinases (pepsin) in the stomach and serine proteinases (trypsin, chymotrypsin, collagenase and elastase) in the intestine and pyloric ceca (Klomklao et al, 2011;Vannabun et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Friedman

Behrens

Pereira

et al. 2021

Journal of Fish Biology

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Fish processing generates plenty of waste that is directly discarded in open-air dumps and water sources, or treated in the same way as urban solid waste, causing serious pollution problems. The waste represents a significant source of high-value bioproducts with potential applications in different industrial processes such as the production of feed, fertilizers, biodiesel and biogas, detergent additives and cosmetics. The objective of this study was to characterize and compare specific activities under different pH values and temperature conditions of acid and alkaline proteinases and viscera yield from the following fish species: Argentine hake Merluccius hubbsi, Brazilian flathead Percophis brasiliensis, Brazilian codling Urophycis brasiliensis and Stripped weakfish Cynoscion guatucupa. Individuals were fished off the coast of Mar del Plata (Argentina) by a commercial fleet and the viscera were immediately extracted and kept on ice until use. Stomach proteinases from four species had the highest activity at pH 2, with stability in the range of pH 2-4. The optimum pH was 11.5 from intestinal enzymes of C. guatucupa, M. hubbsi and P. brasiliensis and 9.5 from intestinal enzymes of U. brasiliensis. Alkaline proteinases from all species were highly stable in the range of 7-11.5. The optimum temperature of stomach proteinases from the four species studied were 30 and 50 C, with stability at 10 and 30 C during 150 min. The optimum temperature of intestinal enzymes from the tested species were 50 C with high stability at 10 and 30 C during 150 min. Alkaline proteinase from all species and acid proteinases from C. guatucupa were inactive at 70 C after 150 min, while there was a residual activity lower than 5% at 80 C on preincubated stomach enzymes of M.hubbsi, P. brasiliensis and U. brasiliensis after 5, 10 and 20 min, respectively. Digestive proteinases recovered in this study could be appropriate for technological usage, reducing manufacturing costs, obtaining revenue from fishery wastes, and contributing to the reduction of environmental pollution.

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“…Proteases are the most widely used group of enzymes in industrial bioprocesses so they represent about 40-65% of the total commercial enzyme preparations (Atta et al 2017). In this sense, sh viscera are one of the most important wastes because they contain digestive enzymes, especially aspartic proteases (pepsin) in stomach, and serine proteases (trypsin, chymotrypsin, collagenase, and elastase) in intestine and pyloric ceca (Klomklao et al 2011;Vannabun et al 2014;Välimaa et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Digestive Proteases From Fish Processing Wastes: Their Partial Characterization and Comparison

Friedman¹,

Behrens²,

Pereira³

et al. 2020

Preprint

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Fish processing generates a lot of wastes which are discarded resulting in environmental problems. However, this material represents a significant source of high-value bioproducts with potential biotechnological applications. The objective of this study was to characterize and to compare specific activities of acid and alkaline proteases recovered from the viscera of Merluccius hubbsi (Mh), Percophis brasiliensis (Pb), Urophyis brasiliensis (Ub), and Cynoscion guatucupa (Cg) under different pH and temperature conditions. Stomach proteinases from four species had a higher activity at pH 2, with stability in the range of pH 2-4. Optimum pH from intestinal enzymes of Cg was 11.5, while for the crude extract of Mh, Pb, and Ub catalytic activity was registered over a wide pH range range from 7 to 11.5. Stomach proteinases from four studied species had a higher activity at 30 °C and 50 °C, with stability at 10 °C and 30 °C. Optimum temperature from intestinal enzymes of the four tested species was 50 °C with high stability at 10 °C and 30 °C. Alkaline proteinase from all species and acid proteinases from Cg was inactivated at 70ºC, while stomach enzymes of Mh, Pb, and Ub had a residual activity lower than 5% at 80 °C after 5, 10 y 20 minutes of pre-incubation, respectively. Digestive proteinases recovered in this study could be used as biocatalysts in industrial processes, reducing costs, adding value to the fishery waste, and contributing to the reduction of environmental pollution.

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Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish

Cited by 4 publications

References 19 publications

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Ultraviolet irradiation effect on characteristics of protease enzyme from African catfish (Clarias sp.) intestines

Digestive proteinases from the marine fish processing wastes of the South‐West Atlantic Ocean: Their partial characterization and comparison

Digestive Proteases From Fish Processing Wastes: Their Partial Characterization and Comparison

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