IgE reactivity to type I collagen and its subunits from tilapia (Tilapia zillii)

Tropomyosin from tilapia (Oreochromis mossambicus) as an allergen

“…By far, most attention has been paid to the main allergens which are the small (approximately 12 kD) parvalbumins. In addition, both collagen from tila-pia (T. zillii) [31] and several other IgE-binding protein from tilapia (O. mossambicus) [32] are considered potential allergens. Parvalbumin from tilapia was previously described by Lee et al [30].…”

“…Ebo et al detected specific IgE binding to 18 kD and 45 kD proteins from tilapia in immunoblot analysis with serum from a patient which did not react to parvalbumin [9]. In addition, both collagen from tila-pia (T. zillii) [31] and several other IgE-binding protein from tilapia (O. mossambicus) [32] are considered potential allergens. The present results from immunoblots using individual sera from ten patients with confirmed tilapia allergy corroborate the finding that a number of different allergens with varying molecular weights are present in O. mossambicus.…”

“…Parvalbumin from tilapia was described by Lee et al [30]. Also, collagen from tilapia (Tilapia zillii) is considered a potential allergen [31]. Also, collagen from tilapia (Tilapia zillii) is considered a potential allergen [31].…”

“…Allergens other than parvalbumin were identified by Ebo et al [9]. Also, collagen from tilapia (Tilapia zillii) is considered a potential allergen [31]. Still other allergens have described by Liu et al, of which two were identified as fructose-bisphosphate aldolase and enolase 3 [32].…”

Investigation of the hazardous substance causing crayfish-induced rhabdomyolysis via a mouse model, a hemolysis assay, and a cytotoxicity assay

“…The enzyme-linked immunosorbent assay (ELISA) was used to measure the sera IgE levels of mice according to the method of Pan et al [8]. The dry muscle samples (0.15 g) and GIG samples of crayfish were ground and mixed with 25 ml PBS buffer (containing 3 % NaCl), respectively.…”