Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

Caro, Isabelle; Boulenc, Xavier; Rousset, Monique; Meunier, Viviane; Bourrié, Martine; Julian, B; Joyeux, H; Roques, Claude; Berger, Yves; Zweibaum, Alain; Fabre, Gérard

doi:10.1016/0378-5173(94)00280-i

Cited by 70 publications

(45 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were monitored as mycoplasma free by polymerase chain reaction (PCR). 12) Caco2/TC7 cells were cultured as described by Caro et al 9) Briefly, 0.5 10 6 cells were seeded on 75 cm 2 plastic flasks (Corning Costar) and the medium was completely changed every other day with Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% of fetal calf serum and 1% of nonessential aminoacids. Caco2/TC7 cells were incubated in a controlled atmosphere at 37°C, 95% relative humidity (RH), with 10% CO 2 medium containing DMEM supplemented with 10% fetal calf serum, 1% nonessential aminoacids, penicillin (110 UI/ml), and streptomycin (110 mg/ml).…”

Section: )mentioning

confidence: 99%

“…Caco2/TC7 cells were incubated in a controlled atmosphere at 37°C, 95% relative humidity (RH), with 10% CO 2 medium containing DMEM supplemented with 10% fetal calf serum, 1% nonessential aminoacids, penicillin (110 UI/ml), and streptomycin (110 mg/ml). Caco2/TC7 cells were incubated in a controlled atmosphere at 37°C, 95% RH, and 10% CO 2 and cultured for 21 to 28 d. 9) For transport studies, 1.5 10 6 cells were seeded on 24-mm diameter TranswellClear ® dishes. The cells were differentiated for 21 to 28 d in the complete medium supplemented with penicillin (110 UI/ml) and streptomycin (110 mg/ml).…”

Section: )mentioning

confidence: 99%

“…In intestinal permeation assays with the enterocyte-like Caco-2 cell line, the maximal DMSO concentration is 1% final concentration. [7][8][9] However, there is little information on the nontoxic and maximal usable concentration in Caco2 cell monolayers in transport studies (incubation time from 2 to 3 h, at 37°C, 10% CO 2 , and 95% relative humidity). Moreover, a previous investigation showed that DMSO could also affect the apparent permeability of lipophilic compounds by underor overestimating their permeability across artificial membranes.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Violante¹,

Zerrouk²,

Richard³

et al. 2002

Biological & Pharmaceutical Bulletin

208

118

View full text Add to dashboard Cite

Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Violante¹,

Zerrouk²,

Richard³

et al. 2002

Biological & Pharmaceutical Bulletin

208

118

View full text Add to dashboard Cite

“…26 The culture conditions for growing Caco-2 cells were the same as described previously. [27][28][29][30] Briefly, after aspirating the culture medium, the cell monolayer was washed thrice with blank HBSS (at pH 7.4 and 37°C).…”

Section: Release Of Ppd From Cubic Nanoparticlesmentioning

confidence: 99%

Enhanced oral absorption of 20(S)-protopanaxadiol by self-assembled liquid crystalline nanoparticles containing piperine: in vitro and in vivo studies

Jin¹,

Zhang²,

Sun³

et al. 2013

IJN

View full text Add to dashboard Cite

Background: 20(S)-protopanaxadiol (PPD), similar to several other anticancer agents, has low oral absorption and is extensively metabolized. These factors limit the use of PPD for treatment of human diseases. Methods: In this study, we used cubic nanoparticles containing piperine to improve the oral bioavailability of PPD and to enhance its absorption and inhibit its metabolism. Cubic nanoparticles loaded with PPD and piperine were prepared by fragmentation of glyceryl monoolein (GMO)/poloxamer 407 bulk cubic gel and verified using transmission electron microscopy and differential scanning calorimetry. We evaluated the in vitro release of PPD from these nanoparticles and its absorption across the Caco-2 cell monolayer model, and subsequently, we examined the bioavailability and metabolism of PPD and its nanoparticles in vivo. Results: The in vitro release of PPD from these nanoparticles was less than 5% at 12 hours. PPDcubosome and PPD-cubosome loaded with piperine (molar ratio PPD/piperine, 1:3) increased the apical to basolateral permeability values of PPD across the Caco-2 cell monolayer from 53% to 64%, respectively. In addition, the results of a pharmacokinetic study in rats showed that the relative bioavailabilities of PPD-cubosome [area under concentration-time curve (AUC) 0-∞ ] and PPD-cubosome containing piperine (AUC 0-∞ ) compared to that of raw PPD (AUC 0-∞ ) were 166% and 248%, respectively. Conclusion: The increased bioavailability of PPD-cubosome loaded with piperine is due to an increase in absorption and inhibition of metabolism of PPD by cubic nanoparticles containing piperine rather than because of improved release of PPD. The cubic nanoparticles containing piperine may be a promising oral carrier for anticancer drugs with poor oral absorption and that undergo extensive metabolism by cytochrome P450.

show abstract

“…Caco-2 cells (TC7 clone), gifted to the Sharp lab by Monique Rousset and Edith BrotLaroche [20], were used from cell passages 46-49. The TC7 clone has been validated for use in studies on iron metabolism [21], and has been used in our previous published work on iron bioavailability [22].…”

Section: Cell Culturementioning

confidence: 99%

Iron bioavailability from commercially available iron supplements

et al. 2014

View full text Add to dashboard Cite

PurposeIron deficiency anaemia (IDA) is a global public health problem. Treatment with the standard of care ferrous iron salts may be poorly tolerated leading to noncompliance and ineffective correction of IDA. Employing supplements with higher bioavailability might permit lower doses of iron to be used with fewer side effects, thus improving treatment efficacy. Here we compared the iron bioavailability of ferrous sulphate tablets with alternative commercial iron products, including three liquid based supplements. MethodsIron bioavailability was measured using Caco-2 cells with ferritin formation as a surrogate marker for iron uptake. Statistical analysis was performed using one-way ANOVA followed by either Dunnett's or Tukey's multiple comparisons tests. Results Spatone Apple® (a naturally iron-rich mineral water with added ascorbate) and IronVital F ® (a synthetic liquid iron supplement) had the highest iron bioavailability.There was no statistical difference between iron uptake from ferrous sulphate tablets, Spatone ® (naturally iron-rich mineral water alone) and Pregnacare Original ® (a multimineral/vitamin tablet). ConclusionIn our in vitro model naturally iron-rich mineral waters and synthetic liquid iron formulations have equivalent or better bioavailability compared with ferrous iron sulphate tablets. If these results are confirmed in vivo, this would mean that at risk groups for IDA could be offered a greater choice of more bioavailable and potentially better tolerated iron preparations.

show abstract

Characterisation of a newly isolated Caco-2 clone (TC-7), as a model of transport processes and biotransformation of drugs

Cited by 70 publications

References 35 publications

Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Enhanced oral absorption of 20(S)-protopanaxadiol by self-assembled liquid crystalline nanoparticles containing piperine: in vitro and in vivo studies

Iron bioavailability from commercially available iron supplements

Contact Info

Product

Resources

About