Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Violante, Georges Da; Zerrouk, Naïma; Richard, Isabelle; Provot, Gérard; Chaumeil, Jean‐Claude; Arnaúd, Philippe

doi:10.1248/bpb.25.1600

Cited by 204 publications

(127 citation statements)

References 16 publications

(23 reference statements)

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“…Although similar studies have been published for Caco-2 cells (Da Violante et al, 2002), the results of such studies may not be extrapolated to the respiratory cells because of differences in cell lineage, morphology, epithelial modeling, rate of cell turnover, and the expression of transporters capable of altering toxicity via efflux mechanisms (Hidalgo et al, 1989;Grainger et al, 2006). Therefore, the aim of this study was to identify the concentration range within which to use the selected solubilizers for in vitro drug delivery studies with respiratory epithelial cells (Calu-3 cells) without alteration of cell membrane integrity and viability.…”

Section: Introductionsupporting

confidence: 71%

Water-soluble organic solubilizers for in vitro drug delivery studies with respiratory epithelial cells: Selection based on various toxicity indicators

et al. 2010

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Section: Introductionsupporting

confidence: 71%

Water-soluble organic solubilizers for in vitro drug delivery studies with respiratory epithelial cells: Selection based on various toxicity indicators

et al. 2010

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“…Other cell types appear to be more resistant to the cytotoxic effects of DMSO than hair cells. For example, DMSO concentration of 10% had no cytotoxic effects on Caco2/TC7 cells (Da Violante et al, 2002).…”

Section: Discussionmentioning

confidence: 95%

Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures

2008

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The amphipathic molecule dimethyl sulphoxide (DMSO) is a solvent often used to dissolve compounds applied to the inner ear; however, little is known about its potential cytotoxic side effects. To address this question, we applied 0.1 to 6% DMSO for 24 h to cochlear organotypic cultures from postnatal day 3 rats and examined its cytotoxic effects. DMSO concentrations of 0.1% and 0.25% caused little or no damage. However, concentrations between 0.5 and 6% resulted in stereocilia damage, hair cell swelling and a dose-dependent loss of hair cells. Hair cell damage began in the basal turn of the cochlea and spread towards the apex with increasing concentration. Surprisingly, DMSO-induced damage was greater for inner hair cells than outer hair cell whereas nearby supporting cells were largely unaffected. Most hair cell death was associated with nuclear shrinkage and fragmentation, morphological features consistent with apoptosis. DMSO treatment induced TUNEL positive staining in many hair cells and activated both initiator caspase-9 and caspase-8 and executioner caspase-3; this suggests that apoptosis is initiated by both intrinsic mitochondrial and extrinsic membrane cell death signaling pathways.

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“…However, little is known about its potential cytotoxic side effects on different cell lines. In addition, the effects of DMSO on cellular function are studied in a large number of cell types with variable results (34)(35)(36)(37). Several studies indicated that proliferation of various cells was obviously inhibited by DMSO at different concentrations (38).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

Jamalzadeh¹,

Ghafoori²,

Sariri³

et al. 2016

Avicenna J Med Biochem

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Background: Organic solvents are widely used in cell biology experiments. Despite increasing the solubility, they have some moderate toxic effects. Therefore, selecting the appropriate solvent along with the use of suitable concentration insures the accuracy and reliability of experimental results. Objectives: The current study aimed to examine the cytotoxic effects of some organic solvents on various cell models including MCF-7, RAW-264.7 and human umbilical vein endothelial cells (HUVEC). Materials and Methods:To evaluate the cytotoxicity effect of common organic solvents on the MCF-7, RAW-264.7 and HUVEC cells, multi-table tournament (MTT) colorimetric assay, the widely used and validated cytotoxicity test was applied. For this purpose, the selected cells were treated with different concentrations (0, 0.1%, 0.5%, 1%, 1.5%, 2%, 3% and 5% v/v) of four most commonly used organic solvents (acetone, ethanol, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) and then subjected to MTT experiment. Results: According to the obtained results, the cytotoxicity increased significantly with increasing the concentration of all four solvents compared to that of the control group. Studies with MCF-7, RAW-264.7 and HUVEC suggested that acetone, ethanol and DMSO at concentrations of 0.1% and 0.5%, had little or no toxicity, whereas higher concentrations inhibited the growth of all three cells. Compared with other three solvents, DMF displayed rather greater toxicity. Based on the results, proliferation of MCF-7, RAW-264.7 and HUVEC cells were inhibited by all used organic solvents, dose dependently. Conclusions: Thus, the background experimental error can be reduced remarkably by maximal concentration of 0.5% ethanol, acetone and DMSO and 0.1% DMF in the final treatment medium.

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Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures.

Cited by 204 publications

References 16 publications

Water-soluble organic solubilizers for in vitro drug delivery studies with respiratory epithelial cells: Selection based on various toxicity indicators

Water-soluble organic solubilizers for in vitro drug delivery studies with respiratory epithelial cells: Selection based on various toxicity indicators

Cytotoxic effects of dimethyl sulphoxide (DMSO) on cochlear organotypic cultures

Cytotoxic Effects of Some Common Organic Solvents on MCF-7, RAW-264.7 and Human Umbilical Vein Endothelial Cells

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