Characterisation and risk factor profiling of Pseudomonas aeruginosa urinary tract infections: pinpointing those likely to be caused by multidrug-resistant strains

Tumbarello, Mario; Raffaelli, Francesca; Peghin, Maddalena; Losito, Angela Raffaella; Chirico, Luigi; Giuliano, Gabriele; Spanu, Teresa; Sartor, Assunta; Fiori, Barbara; Bassetti, Matteo

doi:10.1016/j.ijantimicag.2020.105900

Cited by 13 publications

(17 citation statements)

References 27 publications

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“…Nevertheless, our findings were lower than that found by Karve et al (15% and 18.6%) [ 22 ]. MDR bacteria were not associated with mortality, neither in-hospital mortality nor 30-day mortality, similarly to that found in other studies with younger adults patients [ 25 , 26 ]. Studies on the effects of antibiotic resistance on fitness often document fitness costs of varying severity [ 30 ], and this could explain the no difference in mortality between resistant and non-resistant infections in our study, although there was a difference in appropriate and inappropriate antibiotic treatment.…”

Section: Discussionsupporting

confidence: 89%

“…Lee et al [ 25 ], comparing MDR and non-MDR Enterobacteriaceae causing bacteraemic UTI, described similarly an effectiveness of empirical therapy of 43.5% vs. 93.9%, p < 0.001, and an increase in hospital stay (11.2 ± 6–62 vs. 9.27 ± 5.09 days, p < 0.001). In the same way, Tumbarello et al [ 26 ] described an IEAT of 53.8% vs. 23.3% in a study with MDR and non-MDR P. aeruginosa UTI, as well as a longer hospital stay (48 vs. 22 days, p < 0.001).…”

Section: Discussionmentioning

confidence: 74%

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Clinical impact of multidrug-resistant bacteria in older hospitalized patients with community-acquired urinary tract infection

et al. 2021

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Introduction Previous studies have described some risk factors for multidrug-resistant (MDR) bacteria in urinary tract infection (UTI). However, the clinical impact of MDR bacteria on older hospitalized patients with community-acquired UTI has not been broadly analyzed. We conducted a study in older adults with community-acquired UTI in order to identify risk factors for MDR bacteria and to know their clinical impact. Methods Cohort prospective observational study of patients of 65 years or older, consecutively admitted to a university hospital, diagnosed with community-acquired UTI. We compared epidemiological and clinical variables and outcomes, from UTI due to MDR and non-MDR bacteria. Independent risk factors for MDR bacteria were analyzed using logistic regression. Results 348 patients were included, 41.4% of them with UTI due to MDR bacteria. Median age was 81 years. Hospital mortality was 8.6%, with no difference between the MDR and non-MDR bacteria groups. Median length of stay was 5 [4–8] days, with a longer stay in the MDR group (6 [4–8] vs. 5 [4–7] days, p = 0.029). Inadequate empirical antimicrobial therapy (IEAT) was 23.3%, with statistically significant differences between groups (33.3% vs. 16.2%, p < 0.001). Healthcare-associated UTI variables, in particular previous antimicrobial therapy and residence in a nursing home, were found to be independent risk factors for MDR bacteria. Conclusions The clinical impact of MDR bacteria was moderate. MDR bacteria cases had higher IEAT and longer hospital stay, although mortality was not higher. Previous antimicrobial therapy and residence in a nursing home were independent risk factors for MDR bacteria.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 74%

Clinical impact of multidrug-resistant bacteria in older hospitalized patients with community-acquired urinary tract infection

et al. 2021

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“…(Ronald, 2002, Amdekar et al, 2011, Schaffer & Pearson 2015, Stefaniuk et al, 2016, Staphylococcus spp. (Ronald, 2002, Beyene & Tsegaye, 2011, Amdekar et al, 2011 and Pseudomonas aeruginosa (Ronald, 2002, Todar, 2006, Tumbarello et al, 2020. The study refer to the majority of isolates were Escherichia coli, followed by Klebsiella spp.…”

Section: Cases Distribution With Regard To Age Stagesmentioning

confidence: 90%

“…Klebsiella pneumonia is the species of aerobic Gram-negative bacteria, it is the most relevant human pathogen within genus Klebsiella spp., and it is causing many infections worldwide including urinary tract and it is the most important microorganism causes urinary tract infection, next to Escherichia coli (Cristea et al, 2017) Peudomonas aeruginosa is an opportunist microbe and it is one of urinary tract infection microbes (Tumbarello et al, 2020). It is even the third most common pathogen-related with hospital-acquired catheter-associated UTIs (Jarvis & Martone, 1992).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Relation of Urinary Tract Infection Bacterial Pathogens to Sex and Ages Among Patients in Three Arab Countries

Azab

2021

Al-Azhar Journal of Pharmaceutical Sciences

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Background: urinary tract infections is one of the most prevalent bacterial infections worldwide. The present study aims to study and survey different isolates from urine specimens from different countries to assess their prevalence and their relation to urinary tract infections. One hundred and sixty-eight samples were randomly collected from three countries, Egypt, Sudan and Saudi Arabia, as follows: 43, 33 and 92, respectively. Samples were collected from private laboratories and from both sexes. The ages of the patients differed between three years and 89 years for females and from one year to 85 years for males between 4 / 2015-7 / 2016. All samples were inoculated on different selective and differential sterile culture media. After growth, isolated bacteria were identified by physiological and biochemical characteristic. Among all clinical samples, five bacterial genera were detected. The isolates were identified as Escherichia coli (93), Klebseilla spp. (32), Pseudomonas spp. (26), Proteus spp. (14), and Staphylococcus spp. (3). Regarding the sex of the patient, this study showed that females are more likely to be infected than males, with 53 (31.55%) of the patients being males and 115 (68.45%) of being females. The most common UTI bacterium was Escherichia coli, followed by Klebseilla spp. Majority of female infected cases were in menstruation age stage (14-44 years), while majority males were in old age stage (52-85 years). Surveys and studies of infectious factors are considered one of the most important epidemiological tools for tracking infectious diseases and predicting disease patterns, especially with regard to urinary tract infections due to their widespread prevalence and serious complications between the sexes at the global level.

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“…Its pathogenic characteristics cause secondary infections, occurring mostly when the body's immunity is reduced, such as after extensive burns (Karaky et al., 2020) and following surgery (Richards & Marshall, 2018). P. aeruginosa can also cause human Hospital‐Acquired Pneumonia (Goncalves‐De‐Albuquerque et al., 2016), urinary tract infections (Pachori et al., 2019; Tumbarello et al., 2020), and bacteremia (Callejas‐Díaz et al., 2019; Mccarthy & Paterson, 2017). There are approximately 51,000 medical‐related infections in the United States every year (Cdc, 2019).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Pseudomonas aeruginosa using a DNAzyme‐based sensor

Qin

Fan

et al. 2021

Food Science & Nutrition

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In the present study, a DNAzyme was screened in vitro through the use of a DNA library and crude extracellular mixture (CEM) of Pseudomonas aeruginosa. Following eight rounds of selection, a DNAzyme termed PAE‐1 was obtained, which displayed high rates of cleavage with strong specificity. A fluorescent biosensor was designed for the detection of P. aeruginosa in combination with the DNAzyme. A detection limit as low as 1.2 cfu/ml was observed. Using proteases and filtration, it was determined that the target was a protein with a molecular weight of 10 kDa–50 kDa. The DNAzyme was combined with a polystyrene board to construct a simple indicator plate sensor which produced a color that identified the target within 10 min. The results were reliable when tap water and food samples were tested. The present study provides a novel experimental strategy for the development of sensors based on a DNAzyme to rapidly detect P. aeruginosa in the field.

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Characterisation and risk factor profiling of Pseudomonas aeruginosa urinary tract infections: pinpointing those likely to be caused by multidrug-resistant strains

Cited by 13 publications

References 27 publications

Clinical impact of multidrug-resistant bacteria in older hospitalized patients with community-acquired urinary tract infection

Clinical impact of multidrug-resistant bacteria in older hospitalized patients with community-acquired urinary tract infection

Prevalence and Relation of Urinary Tract Infection Bacterial Pathogens to Sex and Ages Among Patients in Three Arab Countries

Rapid detection of Pseudomonas aeruginosa using a DNAzyme‐based sensor

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