Characterisation and Antifungal susceptibility pattern of Candida species isolated from various clinical samples at a tertiary care centre in South India

Rajeevan, Sumita; Thomas, Marina; Appalaraju, B

doi:10.5958/2394-5478.2016.00014.5

Cited by 10 publications

(13 citation statements)

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“…This makes Candida species identification and susceptibility testing of these isolates mandatory and important. In the present study, 35 isolates (58%) were from samples of IPD patients, 20 isolates (34%) from OPD and 5(8%) from ICU samples which was also seen in a study by Rajeevan et al in which more samples were from IPD as compared to OPD [1]. There was a female predominance among isolates as 42 were from females as compared to 18 from males similar to studies by Mukhia et al and Pawar et al [7,8].…”

Section: Discussionsupporting

confidence: 88%

“…Candida species are part of normal human flora and are opportunists capable of causing a wide spectrum of infections [5,1]. Colonisation of the mucocutaneous surfaces is the first step towards infection.…”

Section: Discussionmentioning

confidence: 99%

“…Alteration in this balance results in growth and subsequent invasion and is supported by various risk factors leading to immunosuppression [5]. Some of these include infection with HIV/AIDS, indiscriminate antibiotic use, use of intravenous catheters, urinary tract catheterisation, hepatic and renal failure, prolonged hospital stay, chemotherapy, organ transplant, leukaemia, diabetes mellitus and Chronic Obstructive Pulmonary Disease (COPD) [1,2,6]. Though infection with C. albicans is common, infection with drug resistant NAC are on the rise over the last few years [3].…”

Section: Discussionmentioning

confidence: 99%

“…Candida species are ubiquitously present as commensals in the human body. In immunocompromised and hospitalized patients, they can cause various types of infections ranging from cutaneous to bloodstream infections and hence are capable of causing morbidity and mortality in patients [1]. The genus comprises of heterogeneous group of organisms out of which 20 different Candida species are known to cause human infections [2].…”

Section: Introductionmentioning

confidence: 99%

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Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Jangla

Naidu

Patel

2018

Int Jour of Biomed Res

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Background: Over the last few years fungal infection rates have increased and a change is seen in their epidemiology and antifungal susceptibility pattern. Hence this study was conducted to learn the distribution of Candida species in various samples and their antifungal susceptibility pattern.Material and Methods: A total of 60Candida isolates were included in the study. Identification was done by colony morphology and Gram stain. Speciation was carried out by Germ-tube test, urease test, chlamydoconidia production test, colony characteristics on chromogenic agar medium, sugar assimilation test,sugar fermentation testand Vitek2 compact(Biomeriux, France) using ID-YST cards. Antifungal testing was done on Vitek2 compact using AST YS01 cards which included fluconazole, voriconazole, amphotericin b, caspofungin, micafungin and flucytosine.Results: 60 Candida isolates were included in this study. Samples from which Candida species were isolated were sputum (45%), urine (33.5%), pus (12%), vaginal swab (5%), endotracheal secretion (1.5%), blood (1.5%) and tissue (1.5%). Isolates from males and females were 30% and 70% respectively. Isolates from geriatric age group (>65 years) and adults (18-65 years) were 52% and 48% respectively. Isolates from samples received from IPD, OPD and ICU were 58%, 34% and 8% respectively. Out of all isolates, Candida albicans was 58%, Candida tropicalis 20%, Candida glabrata 10%, Candida parapsilosis 9% and Candida krusei3%. All Candida species (except Candidaglabrata) showed 100% sensitivity to amphotericin b and caspofungin. Sensitivity to azole group of drugs was 100% among NAC except C. glabrata and C. krusei and more than 90% among C. albicans.Conclusion: Candida albicans was the commonest isolate followed by C. tropicalis among the NAC. Overall also, C. Albicans were predominant as compared to Non albicans Candida (NAC) species. All Candidaisolates except (C. glabrata) showed good sensitivity to all antifungals.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Jangla

Naidu

Patel

2018

Int Jour of Biomed Res

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“…Candida albicans is an opportunistic fungus and a part of the normal gut flora (1,2). Although the microorganism is not normally harmful, overgrowth of the fungus can result in candidiasis and candidemia (3). The fungus is characterized by multiple virulence factors such as biofilm formation and protease production (4).…”

Section: Introductionmentioning

confidence: 99%

Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation

2019

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Background and Objectives: Candida albicans is one of the most common fungal pathogens that can form biofilm, particularly on surface of medical devices. In recent years, C. albicans has shown increased resistance to antifungal agents. In this experimental study, we aimed to study effects of superparamagnetic iron oxide nanoparticles (Fe3O4 nanoparticles or SPION) on biofilm formation by C. albicans. Methods: First, the SPION were synthesized by chemical co-precipitation. The formation of nanoparticles was confirmed by Fourier-transform infrared spectroscopy and Xray diffraction. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of SPION were determined. Then, antibiofilm effects of the nanoparticles were investigated by enzyme-linked immunosorbent assay. Finally, data were analyzed using SPSS 22.0 at significance level of 0.05. Results: According to the results of X-ray diffraction, the SPION had a mean diameter of about 70 nm. MIC and MFC values of SPION against C. albicans were 100 ppm and 200 ppm which reduced biofilm formation by 87.2% and 100%, respectively. SPION showed significant inhibitory effects on C. albicans growth and biofilm formation. Conclusion: Based on the findings, SPION may be considered as a novel family of fungicidal compounds. However, further studies are necessary to evaluate the safety of these nanoparticles for treatment of fungal infections in humans.

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A systematic review on distribution and antifungal resistance pattern of Candida species in the Indian population

et al. 2021

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The emergence of antifungal drug resistance in Candida species has led to increased morbidity and mortality in immunocompromised patients. Understanding species distribution and antifungal drug resistance patterns is an essential step for novel drug development. A systematic review was performed addressing this challenge in India with keywords inclusive of ‘Candida’, ‘Antifungal Drug Resistance’, ‘Candidemia’, ‘Candidiasis’ and ‘India’. A total of 106 studies (January 1978-March 2020) from 20 Indian states were included. Of over 11,429 isolates, Candida albicans was the major species accounting for 37.95% of total isolates followed by C. tropicalis (29.40%), C. glabrata (11.68%) and C. parapsilosis (8.36%). Rates of antifungal resistance were highest in non-albicans Candida (NAC) species - C. haemuloni (47.16%), C. krusei (28.99%), C. lipolytica (28.89%) and C. glabrata (20.69%). Approximately 10.34% isolates of C. albicans were observed to be drug-resistant. Candida species were frequently resistant to certain azoles (ketoconazole-22.2%, miconazole–22.1% and fluconazole–21.8%). In conclusion, the present systematic review illustrates the overall distribution and antifungal resistance pattern of Candida species among the Indian population that could be helpful in the future for the formation of treatment recommendations for the region but also elsewhere.

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Characterisation and Antifungal susceptibility pattern of Candida species isolated from various clinical samples at a tertiary care centre in South India

Cited by 10 publications

References 0 publications

Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

Investigating Effects of Superparamagnetic Iron Oxide Nanoparticles on Candida albicans Biofilm Formation

A systematic review on distribution and antifungal resistance pattern of Candida species in the Indian population

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