Chapter 7. Detection of Damage to DNA Using Electrochemical and Piezoelectric DNA-Based Biosensors

Labuda, Ján

doi:10.1039/9781849732697-00121

Cited by 5 publications

(1 citation statement)

References 82 publications

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“…With respect to electroactivity of the DNA bases, different solid electrodes have been used to study oxidation processes of the bases and damage to DNA [18][19][20][21]. Electrochemical DNA biosensors with immobilized DNA layers on the electrode surface are known as effective tools at the investigation of DNA interactions and damage [22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

et al. 2015

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A DNA-based biosensor is presented that can be applied to the detection of DNA damage caused by UV-C radiation (254 nm) in the presence of CdTe quantum dots (QDs). The sensor is composed of a glassy carbon electrode whose surface was modified with a layer of dsDNA and another layer of CdTe QDs. The response of this sensor is based on (a) the intrinsic anodic signal of the guanine moiety in the DNA that is measured by square-wave voltammetry, and (b) the cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II). Depending on the size of the QDs, they exert a significant effect on the rate of the degradation of dsDNA by UV-C light, and even by visible light. Timedependent structural changes of DNA include opening of the double helix (as indicated by an increase in the redox response of the guanine moiety due to easy electron exchange with the electrode when compared to the original helix state and by an increase in the voltammetric peak current of the hexacyanoferrate(III/II) anion after degradation of the negatively charged DNA backbone on the electrode). The effects of QDs were verified for salmon sperm DNA and calf thymus DNA, and further corroborated by experiments in which DNA solutions were irradiated in the presence of QDs.

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Section: Introductionmentioning

confidence: 99%

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

et al. 2015

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Biosensor with Protective Membrane for the Detection of DNA Damage and Antioxidant Properties of Fruit Juices

Ziyatdinova

Labuda

2012

Electroanalysis

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With the purpose to prepare a DNA biosensor protected with an outer‐sphere membrane against high molecular weight interferences, a carbon film electrode was layer‐by‐layer modified with dsDNA and chitosan. Using cyclic and square‐wave voltammetry and impedance spectroscopy, the oxidative damage of DNA by the hydroxyl and superoxide anion radicals was detected which consists of opening of the helix structure followed by deep DNA chain degradation. The biosensor has been applied to the detection of the antioxidant effect of apple and orange juices. The investigation of the novel biosensor with a protective membrane represents a significant contribution to the field of DNA biosensors utilization.

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An Electrochemical Nanogenosensor for Label Based and Label Free Detection of H. Pylori cagE Gene and Evaluation of DNA Damage Induced by UVC Radiation

Asadzadeh-Firouzabadi¹,

Zare²

2016

J. Electrochem. Soc.

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An electrochemical nanogenosensor was developed based on a gold nanoparticles/graphene -chitosan (AuNPs/GR-CS) ternary nanocomposite for detection of the target DNA of H. pylori cagE gene. The nanogenosensor construction processes were investigated by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) using [Fe(CN) 6 ] 3−/4− as a redox probe. The optimum conditions were found for the immobilization of thiolated probe on the nanogenosensor surface and its hybridization with the target DNA. The nanogenosensor is capable of detecting the target DNA from a one-nucleotide change in the target sequence. In optimum conditions, the two linear calibration ranges of 1.0 × 10 -15 to 1.0 × 10 -12 M and 1.0 × 10 -12 to 1.0 × 10 -6 M with a detection limit of 5.0 × 10 -16 were obtained for the target DNA determination using an EIS method. The EIS and differential pulse voltammetry (DPV) were applied to detect the DNA damage induced by UVC radiation. In the DPV method, the methylene blue (MB) signal was used to detect dsDNA damage which is due to UVC radiation.

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Chapter 7. Detection of Damage to DNA Using Electrochemical and Piezoelectric DNA-Based Biosensors

Cited by 5 publications

References 82 publications

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Biosensor with Protective Membrane for the Detection of DNA Damage and Antioxidant Properties of Fruit Juices

An Electrochemical Nanogenosensor for Label Based and Label Free Detection of H. Pylori cagE Gene and Evaluation of DNA Damage Induced by UVC Radiation

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