An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Hlavata, Lenka; Striesova, Ivana; Ignat, Teodora; Blaskovisova, Jana; Ruttkay-Nedecký, Branislav; Kopel, Pavel; Adam, Vojtěch; Kizek, René; Labuda, Ján

doi:10.1007/s00604-015-1502-z

Cited by 22 publications

(10 citation statements)

References 31 publications

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“…This order follows structural complexity of the dsDNA types. The same trend in calf thymus and salmon sperm dsDNA structural stability has been observed at UV-C irradiated CdTe QDs [31]. 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58…”

Section: Discussionsupporting

confidence: 65%

“…This order follows structural complexity of the dsDNA types. The same trend in calf thymus and salmon sperm dsDNA structural stability has been observed at UV‐C irradiated CdTe QDs .…”

Section: Discussionmentioning

confidence: 92%

“…In the case of PbSe QDs, the synergistic effects of UV irradiation itself, Pb 2+ ions liberated under the irradiation, and the ROS generated in the presence of the PbSe QDs under the UV irradiation were the factors found as inducing the dsDNA damage . In our laboratory we have tested an effect of thiol‐capped CdTe and UV‐C (254 nm) radiation using the DNA biosensors with the glassy carbon electrode as well as biosensing with the QDs and dsDNA in solution phase performing CV, EIS and electrophoretic experiments . Larger (appr.…”

Section: Introductionmentioning

confidence: 99%

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Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

et al. 2017

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Different sensitivity of various types of DNA to damage and mutation requires a study of effects of chemical and physical factors on integrity of individual DNA structures. Oxidation stress induced by photoactivated nanoparticles including quantum dots is considered to be the main mechanism of their potential risks. In this work, spectrophotometric indicators of reactive oxygen species (ROS) 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD−Cl) and α‐diphenyl‐β‐picrylhydrazyl (DPPH), electrochemical DNA‐based biosensors with the thiol‐capped CdS quantum dots (CdS QDs) immobilized over the chromosomal (ct DNA) and plasmid (pUC19) DNA biorecognition layers and agarose gel electrophoresis were used (i) to detect ROS generated at the UV‐C irradiation (λ=254 nm) of CdS QDs and (ii) to assess and evaluate their effect towards the chromosomal and plasmid dsDNA structure. Voltammetric and impedimetric measurements revealed a deep degradation of DNA attached to the glassy carbon electrode surface with a stepwise decrease of the portion of survived pUC19 and ct DNA after 60 s to 2400 s irradiation time up to 47 % and to 32 %, resp., comparing to the portion in the absence of CdS QDs reaching 58 % and 40 %, resp. Agarose gel electrophoresis supported the damage to plasmid and chromosomal DNA demonstrating double strand breaks. A decrease in the stability of DNA types towards degradation by ROS in order pUC19>calf thymus>salmon sperm dsDNA has been found.

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Section: Discussionsupporting

confidence: 65%

“…This order follows structural complexity of the dsDNA types. The same trend in calf thymus and salmon sperm dsDNA structural stability has been observed at UV‐C irradiated CdTe QDs .…”

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

et al. 2017

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“…119 Agarose gel electrophoresis has been used in the evaluation of QDs' stability, 114 in the separation of bare QDs from those conjugated to DNA molecules as well as in the confirmation of the QD-DNA interaction. 20,120,121 Agarose gel electrophoresis can also be used to analyze conjugation with Abs or their fragments and other biomolecules, such as peptides. 21,119 Using this technique, Borghei et al 20 distinguished complexes based on CdTe QDs and wild or mutated DNA sequences.…”

Section: Electrophoresismentioning

confidence: 99%

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

Pereira¹,

Monteiro²,

Albuquerque³

et al. 2019

J. Braz. Chem. Soc.

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Quantum dots (QDs) are semiconductor nanocrystals, which present unique photophysical properties, enabling their application as new fluorescent platforms for biomedical sciences. Colloidal QDs are end-capped with organic or inorganic compounds, not only to prevent their agglomeration but also to provide reaction sites for the attachment of targeting (bio)molecules, nanoparticles or other interfaces, for specific biological purposes. The (bio)conjugation can involve non-covalent or covalent interactions, which can be accomplished through different strategies. The final assembly needs to maintain its chemical and optical stability and biochemical functionality. Although a relative good comprehension of the experimental procedures has been established, the bioconjugation process is still a challenge. The present manuscript aims to review the main (bio)conjugation strategies successfully applied to QDs, describing the steps necessary to prepare stable targeting fluorescent nanoplatforms, as well as some usual methods used to evaluate and optimize this process.

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“…Thus, the oxidative DNA damage contributes to carcinogenesis 24, 25. The DNA biosensors can be used for the detection of reactive oxygen species 25, UV exposure 26, and mutagens 27–29.…”

Section: Introductionmentioning

confidence: 99%

Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

et al. 2016

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Electrochemical sensor has been proposed on the base of glassy carbon electrode (GCE) modified with carbon black (CB) and pillar[5]arene (P[5]A). The characteristics of the peak currents were found to be sensitive to the incubation of the sensor in organic acid and DNA solution. The detection of n×(10−8–10−5) M of organic acid and down to (1–5)×10−18 g of DNA was attributed to the effect of the analytes on the aggregation and relative stability of oxidized and reduced forms of P[5]A. No signal interference with the alkali and alkali‐earth metal ions was found. The electrochemical sensor was tested in the detection of specific DNA interactions, i.e. reactive oxygen species damage and intercalating pharmaceuticals detection.

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An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA

Cited by 22 publications

References 31 publications

Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

Detection of ROS Generated by UV‐C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

(Bio)conjugation Strategies Applied to Fluorescent Semiconductor Quantum Dots

Organic Acid and DNA Sensing with Electrochemical Sensor Based on Carbon Black and Pillar[5]arene

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