Biosensor with Protective Membrane for the Detection of DNA Damage and Antioxidant Properties of Fruit Juices

Abstract

With the purpose to prepare a DNA biosensor protected with an outer‐sphere membrane against high molecular weight interferences, a carbon film electrode was layer‐by‐layer modified with dsDNA and chitosan. Using cyclic and square‐wave voltammetry and impedance spectroscopy, the oxidative damage of DNA by the hydroxyl and superoxide anion radicals was detected which consists of opening of the helix structure followed by deep DNA chain degradation. The biosensor has been applied to the detection of the antioxidant effect of apple and orange juices. The investigation of the novel biosensor with a protective membrane represents a significant contribution to the field of DNA biosensors utilization.

Protective membranes at electrochemical biosensors

“…Recently, the concept of outer-sphere membranes have been used in our laboratory at DNA based biosensors (Ziyatdinova and Labuda 2012). We have prepared biosensors by covering the DNA modifi ed carbon electrode with the layer of Nafi on and chitosan.…”

Voltammetric Measurement of Antioxidant Activity by Prevention of Cu(II)-Induced Oxidative Damage on DNA Bases Using a Modified Electrode

“…Another biosensor prepared by modifying a carbon film electrode with dsDNA and chitosan layer-by-layer coverage could detect O 2 •– and • OH oxidative damage on DNA using CV, SWV, and electrochemical impedance spectroscopy (EIS) methods. The developed method was used to determine the AO effects of commercial apple and orange juices . In the study by Uzunboy et al, the DNA damage was electrochemically investigated using PGEs as a DNA sensor platform in combination with DPV; the interaction of the radical species with DNA in the absence/presence of AOs was detected according to the changes in the G oxidation signal.…”

“…The developed method was used to determine the AO effects of commercial apple and orange juices. 21 In the study by Uzunboy et al, 22 the DNA damage was electrochemically investigated using PGEs as a DNA sensor platform in combination with DPV; the interaction of the radical species with DNA in the absence/presence of AOs was detected according to the changes in the G oxidation signal. In the electrochemical method developed to measure the AO potential and protective effect of Acanthophora red macroalgae collected from the Persian Gulf coast of Bushehr, Iran, a biosensor was designed to prevent DNA damage caused by the Fenton reaction.…”

DNA-based biosensors with polyvinyl alcohol external membrane as a tool for the evaluation of antioxidant activity of white wines

“…It's biodegradable, easy to prepare and film forming (Kim et al, 2008;Srinivasa et al, 2003;Tripathi et al, 2010). Recently, DNA biosensors with external protective membranes have been developed in our laboratory and successfully used to evaluate the antioxidant properties of several beverages, such as fruit juices (Ziyatdinova and Labuda, 2012), coffee, tea and beer (Hlavata et al, 2014). The aim of the present paper is evaluation of the antioxidant activity of various white wines, using voltammetric analytical response of the DNA-based biosensor with external membranes of Nafion (NAF), chitosan (CHIT) and polyvinylalkohol (PVA).…”