Changes of plasma l-arginine levels in spontaneously hypertensive rats under induced hypotension

Methylated arginines in stable and acute patients with coronary artery disease before and after percutaneous revascularization

“…With L-arginine being the major substrate for NO another intriguing explanation of reduced concentrations in STEMI patients is the possibility that L-arginine is linked to the occurrence of myocardial infarction. This is supported by findings in hypercholesterolemic stroke-prone spontaneously hypertensive rats suggesting that NO deficiency induces myocardial infarction [14] and by another study in spontaneously hypertensive rats demonstrating a decrease in plasma L-arginine concentration following acute reduction in blood pressure [15]. In a human study of the vasodilatory response to coronary L-arginine infusion, dilation was more pronounced in complex stenoses than smooth stenoses in agreement with local deficiency of L-arginine at locations prone to myocardial Values are mean and (SEM).…”

A fully automated amino acid analyzer using NBD‐F as a fluorescent derivatization reagent

“…Quantitative analysis of amino acids is performed in many areas of science, such as biochemistry, clinical medicine and food science (Bergquist et al, 1994;Zarkadas et al, 1997;Fountoulakis and Lahm, 1998;Tucci et al, 1998;Prados et al, 1999). In these fields, a highly sensitive and simultaneous quantification method for various amino acids is required.…”

Extracellular Arginine Rapidly Dilates In Vivo Intestinal Arteries and Arterioles Through a Nitric Oxide Mechanism

“…The in vivo microvascular efficacy of low concentrations of arginine was shown in animal studies by Frame et al [ 23 , 24 ]. They reported skeletal muscle arterioles of the rat rapidly dilated to local application of 100 μ M L‐arginine, which is just slightly higher or very near plasma concentrations of 50–100 μ M generally reported for adult rats [ 32 , 33 , 42 , 53 , 57 , 58 ], and the dilation was suppressed by inhibition of eNOS. In the current study with the small intestinal vasculature of rats, 200 and 400 μ M L‐arginine increased blood flow within three minutes, and the increased [NO] and diameter of arterioles generally approached 90% of the new and larger steady state within about 60 seconds of adding 400 μ M L‐arginine to the topical bath (Figure 1).…”

“…Most studies [ 9 , 14 , 18 , 22 , 37 , 40 , 54 , 55 , 56 ] of the acute effects of arginine on endothelial production of nitric oxide in vivo or in vitro have used topical concentrations more than ten times the plasma concentration of 50–75 μ M [ 32 , 33 , 42 , 53 , 57 , 58 ]. Part of the rationale for using high concentrations of arginine for testing is that the intracellular L‐arginine concentration range is 100 μ M to 800 μ M [ 2 , 3 , 28 , 34 ] and even higher in freshly isolated endothelial cells [ 34 ].…”