A fully automated amino acid analyzer using NBD‐F as a ﬂuorescent derivatization reagent

Aoyama, Chiaki; Santa, Tomofumi; Tsunoda, Makoto; Fukushima, Takeshi; Kitada, Chieko; Imai, Kazuhiro

doi:10.1002/bmc.365

Cited by 72 publications

(56 citation statements)

References 31 publications

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“…The LODs and LOQs of NBD-amino acids were 0.6-17 fmol/injection and 1.9-57 fmol/injection, respectively. These values are similar with those reported in previous studies with monolithic silica columns [10], particle packed columns [14], and core-shell particle columns [15].…”

Section: Validation Datasupporting

confidence: 92%

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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In this study, the application of a new type of monolithic silica column to the analysis of amino acids in human plasma samples has been investigated. The monolithic silica column (3.0 × 150 mm, MonoTower C18) provided good separation of the amino acids derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). Although the separation ability of this column was not much improved in comparison with the previous monolithic silica columns, it was similar with that of a 3 μm particle packed column. The column with length 150 mm had the same separation property as a tandem connection of columns of lengths 50 mm and 100 mm. After 1000 injections of human plasma samples, the peak widths for the NBD-amino acids were increased by only 8%. Thus, the newly developed monolithic silica column can be potentially applied for faster analysis and/or more efficient separation of biological compounds.

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Section: Validation Datasupporting

confidence: 92%

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

2017

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“…Since the derivatives of NBD-F emit longer-wavelength fluorescence, NBD-F provides higher sensitivity than OPA in most cases. 21,22 Furthermore, we found out that the fluorescence intensity of NBD-Se-Met was enhanced by oxidation reaction.…”

Section: Introductionmentioning

confidence: 86%

Determination of Selenomethionine by High-Performance Liquid Chromatography–Fluorescence Detection Coupled with On-line Oxidation

Aoyama¹,

Tsunoda²,

Funatsu³

2009

ANAL. SCI.

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“…Derivatization was performed as described previously (Aoyama et al, 2004;Zhang et al, 2015), and HPLC analyses were done according to Tan et al (2010). Total amounts of soluble seed proteins were determined according to Zhang et al (2015).…”

Section: Collection Of Leaf Exudates and Xylem Sapmentioning

confidence: 99%

Improving Plant Nitrogen Use Efficiency through Alteration of Amino Acid Transport Processes

2017

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Improving the efficiency of nitrogen (N) uptake and utilization in plants could potentially increase crop yields while reducing N fertilization and, subsequently, environmental pollution. Within most plants, N is transported primarily as amino acids. In this study, pea (Pisum sativum) plants overexpressing AMINO ACID PERMEASE1 (AAP1) were used to determine if and how genetic manipulation of amino acid transport from source to sink affects plant N use efficiency. The modified plants were grown under low, moderate, or high N fertilization regimes. The results showed that, independent of the N nutrition, the engineered plants allocate more N via the vasculature to the shoot and seeds and produce more biomass and higher seed yields than wild-type plants. Dependent on the amount of N supplied, the AAP1-overexpressing plants displayed improved N uptake or utilization efficiency, or a combination of the two. They also showed significantly increased N use efficiency in N-deficient as well as in N-rich soils and, impressively, required half the amount of N to produce as many fruits and seeds as control plants. Together, these data support that engineering N allocation from source to sink presents an effective strategy to produce crop plants with improved productivity as well as N use efficiency in a range of N environments.Nitrogen (N) is an essential nutrient that plants require in large amounts for growth and development. In industrial countries, high N fertilization enables maximum crop yields, and in the last 50 years, the use of synthetic N fertilizers has increased dramatically to meet the agricultural demands of a growing population (FAO, 2012;Conant et al., 2013). However, depending on the crop species and soil conditions, plants take up less than half of the applied N fertilizer (Raun and Johnson, 1999;Hodge et al., 2000;Kumar and Goh, 2002;Yang et al., 2015;Zhu et al., 2016). The remaining soil N may contaminate aquatic systems through runoffs or leached water (Crews and Peoples, 2005;Gruber and Galloway, 2008) or it may undergo denitrification and be released into the atmosphere as nitrous oxide, a powerful greenhouse gas (Bouwman, 1996;Bouwman et al., 2002), altogether resulting in negative effects on the environment and human health. Oppositely, in developing countries, access to N fertilizer is limited, and insufficient N nutrition results in low crop productivity and, ultimately, in reduced food supply (Brown et al., 2009;Lal, 2009).One solution to these issues is the production of crop varieties that are highly efficient in using N and produce high yields with reduced N input (MasclauxDaubresse et al., 2010;McAllister et al., 2012;Garnett et al., 2015). Plant nitrogen use efficiency (NUE) is determined by plant seed yield relative to the amount of N applied and is generally composed of both N uptake and N utilization efficiency (Moll et al., 1982). Nitrogen uptake efficiency (NUpE) is defined as total shoot N relative to the amount of N supplied to the soil. The uptake of N by the root is influenced by th...

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A fully automated amino acid analyzer using NBD‐F as a ﬂuorescent derivatization reagent

Cited by 72 publications

References 31 publications

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Amino Acid Analysis Using a Cartridge-Type Monolithic Silica Column

Determination of Selenomethionine by High-Performance Liquid Chromatography–Fluorescence Detection Coupled with On-line Oxidation

Improving Plant Nitrogen Use Efficiency through Alteration of Amino Acid Transport Processes

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