Natural products have been used in various ways for the promotion of health and the treatment of diseases since ancient times. In particular, polyphenolic compounds contained in natural products offer various physiological activities such as antioxidant, cancer cell growth inhibition, and antimutation, and many studies are being conducted to examine the possibility of using them as therapeutic and preventive agents for cardiovascular diseases and cancer. Camellia japonica has been studied on various physiological activities of leaves, flowers, and seed extracts. However, there has been little research on the antibacterial activity of C. japonica extract, and the effect on antibacterial activity is different depending on the heat-treated process. Therefore, in this study, extracts of heat-treated C. japonica leaves were prepared using hot water, 70% EtOH, and 100% EtOH solvents, and each extract was compared through antioxidant activities (DPPH and ABTS), total polyphenol contents (TPC), and total flavonoid contents (TFC). The 70% EtOH extract showed the best activity, and DPPH and ABTS radical scavenging IC 50 were 262.99 µg/mL and 319.97 µg/mL. TPC also showed the highest content at 167.88 GAE mg/g. However, TFC con-firmed that the contents of each extract were similar. As a result of HPLC analysis, it was confirmed that polyphenols and vitamins showed the highest content in the 70% EtOH extract, and it is thought that there is a need for additional research through more subdivided separation and fractionation. As a result of the disk diffusion test, the activities of Staphylococcus epidermidis and Malasseiza pachydermaits were the best in the 70% EtOH extract. C. Japonica leaves are thought to be very suitable as a functional cosmetic raw material.