Antioxidant Activity and Bacterial Skin Infections Growth Inhibitory Effect of Heat-treated Camellia japonica Leaf Extract

Abstract

Natural products have been used in various ways for the promotion of health and the treatment of diseases since ancient times. In particular, polyphenolic compounds contained in natural products offer various physiological activities such as antioxidant, cancer cell growth inhibition, and antimutation, and many studies are being conducted to examine the possibility of using them as therapeutic and preventive agents for cardiovascular diseases and cancer. Camellia japonica has been studied on various physiological activities of leaves, flowers, and seed extracts. However, there has been little research on the antibacterial activity of C. japonica extract, and the effect on antibacterial activity is different depending on the heat-treated process. Therefore, in this study, extracts of heat-treated C. japonica leaves were prepared using hot water, 70% EtOH, and 100% EtOH solvents, and each extract was compared through antioxidant activities (DPPH and ABTS), total polyphenol contents (TPC), and total flavonoid contents (TFC). The 70% EtOH extract showed the best activity, and DPPH and ABTS radical scavenging IC 50 were 262.99 µg/mL and 319.97 µg/mL. TPC also showed the highest content at 167.88 GAE mg/g. However, TFC con-firmed that the contents of each extract were similar. As a result of HPLC analysis, it was confirmed that polyphenols and vitamins showed the highest content in the 70% EtOH extract, and it is thought that there is a need for additional research through more subdivided separation and fractionation. As a result of the disk diffusion test, the activities of Staphylococcus epidermidis and Malasseiza pachydermaits were the best in the 70% EtOH extract. C. Japonica leaves are thought to be very suitable as a functional cosmetic raw material.

Antioxidant and Anti-obesity Activities of Roasted Camellia japonica Leaves Extract

“…기존의 연구에서 Kim 등은 무처리군과 덖음 (3, 5, 7분) 무말랭이 차의 항산화 활성을 비 교하였는데 덖음 시간이 늘어날수록 높은 활성이 나타남을 보고하였다 [8]. 또한 추출 용매를 달리한 동백나무 덖음 잎 에 대해 70% EtOH 추출물이 항산화 및 피부질환 유발균에 비교적 높은 활성을 가진다고 보고하였다 [10]. 동백 겨우살 이에서 추출한 메탄올과 에탄올 추출물이 라이페이스 (lipase) 와 알파-글루코시데이스 (α-glucosidase)를 억제하는 활성을 보여, 항비만 효과가 있다는 연구 결과가 보고되었다 [11].…”