Changes in uterine RNA during early pregnancy in the rat

Heald, P. J.; O'grady, J. E.; O’Hare, Anne; Vass, Maureen

doi:10.1016/0005-2787(72)90219-5

Cited by 12 publications

(3 citation statements)

References 13 publications

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“…The template activity of uterine chromatin which is high on Day 5 of preg¬ nancy and at oestrus (O'Grady et al, 1975) is enhanced by oestradiol and progesterone (Teng & Hamilton, 1968;Glasser & Clark, 1975 , and specific nonhistone chromosomal proteins, which may be important for gene transcription, are detected in uterine cells (Cohen & Hamilton, 1975). New species of nuclear RNA have been found on Day 5 of pregnancy in the rat (Heald & O'Hare, 1973). Hitherto, consideration has mainly been of the early production of a few cytoplasmic proteins, such as the induced protein (Gorski, 1973;Somjen, Somjen, King, Kaye & Lindner, 1973) postulated to bind to the chromatin to bring about further changes in gene expression (see Cohen & Hamilton, 1975).…”

Section: Discussionmentioning

confidence: 97%

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Surani¹

1977

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Radiolabelled uterine luminal proteins were obtained 6 h after instillation of 5--10 muCi [35S]methionine into the lumen of each uterine horn of rats. The proteins were analysed electrophoretically on 6% polyacrylamide gels and incorporation of the precursor into the separated proteins was estimated in 0-18 cm gel slices by scintillation spectrometry. Major incorporation was obtained on Day 5 of pregnancy in a group of proteins of approximate mol. wt 3 X 10(4), 4-5 X 10(4) and 5-5 X 10(4) and in proteins of mol. wt 8--10 X 10(4). During pro-oestrus, substantial incorporation of the precursor occurred in a group of protein of mol, wt 13-5 X 10(4), 12-5 X 10(4) and 7-5--8-0 X 10(4) and slight incorporation in proteins of mol. wt 4-5 X 10(4). These changes were mimicked in ovariectomized females by injections of appropriate steroids: progesterone and oestradiol were necessary to induce the proteins detected during pregnancy, oestradiol alone was able to reproduce the changes observed at pro-oestrus. The proteins in the lumen appear to be of uterine origin because radiolabelled serum proteins were not detected in the lumen after an i.p. injection of the isotope. Also, labelled proteins were detected in the horn receiving the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope but not in the contralateral horn injected with saline. Cycloheximide, given i.p. simultaneously with the isotope, suppressed synthesis of uterine proteins.

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Section: Discussionmentioning

confidence: 97%

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Surani¹

1977

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“…The appearance of similar nucleolar changes in pregnant rats (Lundkvist andNilsson, 1982,1984) and a period of enhanced uridine incorporation (Heald et al, 1975) are temporally correlated. Implantation and decidual induction by oil are also accompanied by enhanced synthesis of ribosomal RNA (Heald et al, 1972;Miller, 1973). Lundkvist and Nilsson (1984) have suggested that this alteration reflects a n early stage in stromal cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effect of indomethacin and prostaglandin E₂ on structural differentiation of rat endometrium during artificially induced decidualization

Keys

Kennedy

1990

Am. J. Anat.

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Morphological differentiation of uterine stromal and luminal epithelial cells was studied in steroid-injected ovariectomized rats following unilateral intrauterine instillation of sesame oil, phosphate-buffered saline containing gelatin (PBSG), PBSG + indomethacin (IM; an inhibitor of prostaglandin synthesis), or PBSG + IM + prostaglandin E2 (PGE2). The latter two treatments were preceded by a subcutaneous injection of IM. Uteri were examined by light and electron microscopy at intervals between 8 and 120 hr (n = 4/treatment/time). Differentiation began in the periluminal antimesometrial region and progressed peripherally and towards the mesometrial aspect in all groups. Structural features and timing of differentiation were similar for oil-injected and PBSG-infused uteri. Administration of IM inhibited the onset of the decidual cell reaction and had deleterious effects on the luminal epithelium. Inclusion of PGE2 in the instillate accelerated stromal cell differentiation and overcame the inhibitory effect of IM. The results implicate prostaglandins, particularly PGE2, in endometrial transformation during decidualization.

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“…By 10.00 hours on Day 5 of pregnancy there is an increased synthesis of 130 P. J. Heald et al uterine RNA (Heald & O'Grady, 1970), accompanied by an increased rate of processing of nuclear RNA (Heald et al, 1972a) and an increased synthesis of ribosomal RNA (Heald et al, 1972b) which almost ceases by 10.00 hours on Day 6 if implantation and decidualization do not occur. Further, the uterus on Day 5 by 09.00 hours contains a new species of RNA not detectable in decidualizing tissue on Day 7 or in the uterus on Day 2 (Heald & O'Hare, 1973).…”

Section: Introductionmentioning

confidence: 94%

Nucleic Acid Metabolism of Cells of the Luminal Epithelium and Stroma of the Rat Uterus During Early Pregnancy

Heald¹,

O'grady²,

O’Hare³

et al. 1975

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The uptake of [5-3H]uridine into RNA and DNA of the cells of the uterine luminal epithelium, stroma and myometrium of the rat has been studied in early pregnancy using a technique for separation of the cell fractions before quantitative analysis. Comparisons of the metabolism between the pregnant and pseudopregnant horn of the unilaterally ovariectomized rat has shown that RNA and DNA synthesis are markedly increased by 04.00 hours on the morning of Day 5 in the pregnant horn. This increased metabolism occurs in all cell fractions and before the zona pellucida is shed. The results are discussed in relation to the onset of the decidual response.

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Changes in uterine RNA during early pregnancy in the rat

Cited by 12 publications

References 13 publications

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Effect of indomethacin and prostaglandin E₂ on structural differentiation of rat endometrium during artificially induced decidualization

Nucleic Acid Metabolism of Cells of the Luminal Epithelium and Stroma of the Rat Uterus During Early Pregnancy

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Changes in uterine RNA during early pregnancy in the rat

Cited by 12 publications

References 13 publications

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Radiolabelled rat uterine luminal proteins and their regulation by oestradiol and progesterone

Effect of indomethacin and prostaglandin E2 on structural differentiation of rat endometrium during artificially induced decidualization

Nucleic Acid Metabolism of Cells of the Luminal Epithelium and Stroma of the Rat Uterus During Early Pregnancy

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Effect of indomethacin and prostaglandin E₂ on structural differentiation of rat endometrium during artificially induced decidualization