Changes in Type I and Type II Collagen Expression in Rat Mandibular Condylar Cartilage Associated with Aging and Dietary Loading

Orajärvi, Marko; Laaksonen, Sakari; Hauru, Riikka; Mursu, Eerika; Jonaviciute, Egle; Voipio, Hanna‐Marja; Raustia, Aune; Pirttiniemi, Pertti

doi:10.11607/ofph.1581

Cited by 9 publications

(13 citation statements)

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“…Osteoarthritis can affect several joints, including the TMJ. The mandibular condylar cartilage (MCC) of the TMJ is a fibrocartilaginous tissue which contains both type I and type II collagen (Orajarvi et al 2018;Wadhwa and Kapila 2008). The major role of the MCC is to support and distribute functional loads, allowing a frictionless motion and avoiding the breakdown of the cartilage.…”

Section: Introductionmentioning

confidence: 99%

Age-related changes in the cartilage of the temporomandibular joint

et al. 2020

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Osteoarthritis (OA) of the knee is closely associated with aging; however, little is known about the age-related degeneration in the mandibular condylar cartilage (MCC) of the TMJ. Our objective was to examine whether a correlation exists between aging and degeneration of the MCC of the TMJ. Thirty-two male C57BL/6J wild-type mice were aged to 2, 12, 18, and 25 months old. The mice were euthanized by CO 2 inhalation and were dissected and examined by micro-CT and histology. Sagittal sections of the condyles were stained for tartrate-resistant alkaline phosphatase, alkaline phosphatase, safranin O, picrosirius red, and toluidine blue. In addition, immunostaining for BMP2, BMP4, BMP7, PRG4, and MMP13 was performed. Bone volume fraction and tissue density significantly increased with the age of the animals. There was a significant increase in the Osteoarthritis Research Society International histopathological score and mineralization of the noncalcified cartilage in the aged animals. There was a decrease in cartilage thickness, proteoglycan distribution, and cellularity in the aged animals. Additionally, we noted increased picrosirius red staining with the increase in the age of the animals. Our protein expression showed increased BMP2, BMP4, BMP7, and MMP13, whereas there was a decrease in PRG4 expression in the aged animals. As the animal ages, there is decreased proteoglycan secretion, decreased cellularity, decreased cartilage thickness, increased fibrillation, and increased proteolytic activity. A better understanding of the basic mechanisms underlying the degeneration of the MCC in the older animals could provide novel ways to slow the development of OA.

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Section: Introductionmentioning

confidence: 99%

Age-related changes in the cartilage of the temporomandibular joint

et al. 2020

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“…Other models of reduced masticatory activity have been established by changing only the consistency of the diet, i.e. use of a powdery, paste or liquid diet [11,[13][14][15][16][17][18][19]. Bruxism is a normal activity for rodents, in order to keep the incisors sharp.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, few investigations have examined the relationship between food consistency and the TMJ response in models involving older animals. Orajärvi et al [18] reported that the characteristics of the TMJ condylar cartilage were correlated with loading, aging, and sex in older rats. In contrast, no studies have investigated the relationship between condylar bony change and food consistency in models involving older animals.…”

Section: Introductionmentioning

confidence: 99%

Newly developed mastication activity reduction procedure rapidly induces abnormal atrophic change of the mandibular condyle in young and elder experimental animal models

et al. 2020

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This study was performed to develop a new rat model of reduced masticatory activity in order to assess the effect of this reduction on the morphology of the temporomandibular joint (TMJ) over time. Female rats were used, and ovariectomy was performed to simulate aged/ postmenopausal status. Twenty-four SD rats aged 6 weeks were divided into four groups: ovariectomy/sham procedure (Ov/S); ovariectomy/ reduced masticatory activity (Ov/RMA); non-Ov/S (NO/S); and non-Ov/ RMA (NO/RMA). The RMA procedure involved grinding down the edges of the upper and mandibular incisors by about 3 mm and supplying the rats with a powdered diet. The bilateral TMJ was examined by microcomputed tomography at 0, 1, 2, 4, 6, and 8 weeks after the start of RMA. Condylar width was greater in the NO/S group than in the Ov/S group after the 2nd week, showing that ovariectomy reduced the width of the condyle. After the 2nd week, significant differences in condylar width were apparent between the NO/S and NO/RMA groups, and between the Ov/S and Ov/RMA groups. This RMA procedure appeared to provide a good model of reduced masticatory activity. The present findings in female rats suggest that reduction of appropriate mastication activity in the growth period results in poor growth of the mandibular condyle and immediately induces atrophy of the mandibular condyle under conditions simulating aged/postmenopausal status.

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“…Three different measurements for the MCC thickness were made, one in the middle of the thickest portion of the middle third, and one 200 µm anterior and one 200 µm posterior to this line. The number of cartilage cells was measured by counting the cells that intersected these three lines [28]. The result of three measurements per section of condyle 100× magnification resulted in a total of 6 measurements per animal, and the mean value was then calculated.…”

Section: Group 4 (C/g)mentioning

confidence: 99%

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

Khaleel¹,

Shaari²,

Nawi³

et al. 2021

Arch Med Sci Civil Dis

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Introduction: Ciprofloxacin is a broad-spectrum antibiotic which can cause a chondrotoxic effect in the growing condyle. Researchers found that green tea showed significant health benefits for a large number of disorders. The objective of the present research was to investigate the preventive potential of green tea extract against mandibular chondrotoxicity induced by ciprofloxacin in juvenile Wistar rats. Material and methods: Twenty juvenile male rats were used and divided into four equal groups, the saline/water (S/W), saline/green tea (S/G), ciprofloxacin/water (C/W) and ciprofloxacin/green tea (C/G) treated groups. On day 32 of age, all the animals in C/W and C/G treated groups were subcutaneously injected with ciprofloxacin as two subcutaneous injections of 600 mg/kg of body weight, eight hours apart, while the S/W and S/G groups were subcutaneously injected with saline. The S/G and C/G groups were intragastrically gavaged by green tea extract in an oral dose of 300 mg/kg/ day, 8 days before the subcutaneous injection of saline or ciprofloxacin. On day 34, all the animals were anaesthetized, and the mandibular condyle samples were taken immediately and processed. Results: In comparison with the C/W treated group, the C/G treated group showed a significant increase in the thickness of the mandibular condylar cartilage, cartilage cell number, glycosaminoglycan content, and Bcl-2 immune expression (p < 0.05). Conclusions: This study provides the first evidence that green tea extract can significantly decrease the chondrotoxic effects of ciprofloxacin in a rat mandibular condyle cartilage model.

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Changes in Type I and Type II Collagen Expression in Rat Mandibular Condylar Cartilage Associated with Aging and Dietary Loading

Abstract: Condylar cartilage is sensitive to the interplay between loading, aging, and sex of middle-aged and older rats. High loading of condylar cartilage increased the thickness of cartilage in younger rats.

Cited by 9 publications

References 0 publications

Age-related changes in the cartilage of the temporomandibular joint

Age-related changes in the cartilage of the temporomandibular joint

Newly developed mastication activity reduction procedure rapidly induces abnormal atrophic change of the mandibular condyle in young and elder experimental animal models

Assessment of the protective effect of green tea extract against ciprofloxacin-induced chondrotoxicity in albino rats (histochemical, and immunohistochemical study)

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