Changes in plasma miR-9, miR-16, miR-205 and miR-486 levels after non-small cell lung cancer resection

Sromek, Maria; Głogowski, Maciej; Chechlińska, Magdalena; Kulińczak, Mariusz; Szafron, Lukasz; Zakrzewska, Klara; Owczarek, Joanna; Wisniewski, Piotr; Wlodarczyk, Robert A.; Talarek, Lukasz; Turski, Maciej; Siwicki, Jan Konrad

doi:10.1007/s13402-017-0334-8

Cited by 57 publications

(45 citation statements)

References 37 publications

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“…This result was similar to some studies which monitored different microRNAs in serum samples [29,30]. The stimulatory of miR-9 in cancer progression is not only restricted to gastric cancer, since other studies also suggested that the expression of this miRNA could be altered in other human malignancies, including squamous cell carcinoma [31], breast cancer [32] and non-small-cell lung cancer [33]. Wan-Cheng Xiong et al has demonstrated that the up-regulation of miR-9 in colon cancer cells is coupled with the suppression of tumor growth and cancer progression [23].…”

Section: Discussionsupporting

confidence: 89%

Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

2020

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Objective: It has been indicated that there is a tight association between cancer and different factors, such as environment and genetics, including aberrantly expressed microRNAs. The crucial role of microRNAs in the regulation of diverse signaling pathways in gastrointestinal cancer has been established in several studies. In this study, we aimed to evaluate the expression of microRNA-9 and -192 in colon and gastric cancers. After extracting the RNA from tissues and serum samples of patients, suffering from colon and gastric cancer, cDNA was synthesized. Then by performing quantitative real-time PCR, we evaluated the expression level of miR-9-5p and miR-192-5p in collected samples.Results: Unlike to colon cancer in which the expression level of miR-9-5p remained unchanged, the relative expression of this miRNA decreased remarkably in gastric cancer (with P value < 0.05), in comparison with normal adjacent tissues. In agreement with this finding, we also found that the expression level of miR-192-5p was decreased in gastric cancer tissues, compared to normal gastric tissue. Given the reduction in the expression level of miR-9-5p and miR-192-5p in gastric cancer, it could be postulated to consider these miRNAs as promising diagnostic biomarkers.

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Section: Discussionsupporting

confidence: 89%

Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

2020

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“…Increased expression of miR-223 in NSCLC sputum samples was identified as a non-invasive diagnostic biomarker for NSCLC detection (29). The ectopic expression of miR-9, miR-16, miR-205 and miR-486 in serum specimens collected from patients with NSCLC served as useful diagnostic biomarkers for NSCLC (30). Upregulated expression of miR-411 was shown to have relatively high diagnostic and prognostic value for patients with NSCLC (31).…”

Section: Discussionmentioning

confidence: 99%

Ultrasound‑targeted microbubble destruction‑mediated miR‑767 inhibition suppresses tumor progression of non‑small cell lung cancer

et al. 2020

Exp Ther Med

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MicroRNAs (miRNAs/miRs) have important roles in tumor progression in various human cancers. Ultrasound-targeted microbubble destruction (UTMD)-mediated gene transfection has been considered a useful tool for improving cancer treatment. The present study aimed to investigate the role of miR-767 in non-small cell lung cancer (NSCLC) and further analyze the effects of UTMD-mediated miR-767 inhibition on tumor progression. The expression of miR-767 was measured by reverse transcription-quantitative PCR. UTMD-mediated miR-767 inhibition was achieved by the co-transfection of microbubbles and miR-767 inhibitor in NSCLC cells. Cell proliferation was assessed by a CCK-8 assay and cell migration and invasion were examined by a Transwell assay. The expression of miR-767 was increased in NSCLC serum, tissues and cells compared with controls. The reduction of miR-767 in NSCLC cells led to the inhibition of cell proliferation, migration and invasion. UTMD increased the transfection efficiency of the miR-767 inhibitor in NSCLC cells, and UTMD-mediated miR-767 inhibition resulted in a more significant suppressive effect on tumor cell proliferation, migration and invasion. Taken together, the results indicated that miR-767 expression is upregulated in both NSCLC clinical samples and cells. The downregulation of miR-767 can inhibit tumor cell proliferation, migration and invasion, and these effects are further promoted by UTMD-mediated miR-767 inhibition, which indicated the potential of a UTMD-mediated miR-767 inhibition as a novel therapeutic strategy for NSCLC treatment.

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“…Li and their team found that miR-9 promotes cell growth and metastasis in LUAD through the repression of TGFBR2 [42], and may serve as biomarker and predicted the prognosis of LUAD. Increase in plasma miR-9 and decrease in miR-486 levels after NSCLC resection may indicate a good prognosis [43]. These data supported that miR-9 and miR-486 may be key therapeutic targets that were related to LUAD malignancy and outcome, while in-depth studies of their operating mechanism are still await for further exploration.…”

Section: Discussionmentioning

confidence: 63%

Identification of Stage-associated MicroRNAs in Lung Adenocarcinoma Based on Microarray Data

Wang

Gao

et al. 2020

Preprint

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Background: Lung adenocarcinoma (LUAD) is the most aggressive and most frequently seen histological variant of lung cancers, comprising nearly 45% of the overall cases of lung carcinoma and its incidence has been increased significantly worldwide in the last several decades. However, there was limited available evidence with respect to the signalling pathways and miRNAs related to the LUAD.Methods: To identify the differentially-expressed miRNAs (DE-miRNAs) in different stages of LUAD, we performed a microarray on 514 LUAD and 54 normal tissues. At the mean time, the potentially targeted genes as well as the highly-enriched signaling pathways and the relevant protein–protein interaction (PPI) network were analyzed and constructed by using a series of bioinformatic methods. Moreover, the identified DE-miRNAs in different stages of the LUAD were verified by using the TCGA dataset.Results : Overall 41 down-regulated -and 82 up-regulated DE-miRNAs were identified, of which 1,716 potential targeted genes were selected. Moreover, the enriched pathways of the above genes were screened by using the GO term and KEGG pathway analyses, including the AMPK signalling pathway, FoxO signalling pathway, MAPK signalling pathway, PI3K-Akt signalling pathway and hippo signalling pathway.Conclusions: Our study could provide additional understanding of the underlying molecular events and increase the precision of prognostic prediction.

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Changes in plasma miR-9, miR-16, miR-205 and miR-486 levels after non-small cell lung cancer resection

Abstract: Our data indicate that miR-9, miR-16, miR-205 and miR-486 may serve as NSCLC biomarkers. The observed cancer-related pre- and post-operative changes in their plasma levels may not only reflect the presence of a primary cancer, but also of a systemic response to cancer.

Cited by 57 publications

References 37 publications

Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

Ultrasound‑targeted microbubble destruction‑mediated miR‑767 inhibition suppresses tumor progression of non‑small cell lung cancer

Identification of Stage-associated MicroRNAs in Lung Adenocarcinoma Based on Microarray Data

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