Ultrasound‑targeted microbubble destruction‑mediated miR‑767 inhibition suppresses tumor progression of non‑small cell lung cancer

Abstract

MicroRNAs (miRNAs/miRs) have important roles in tumor progression in various human cancers. Ultrasound-targeted microbubble destruction (UTMD)-mediated gene transfection has been considered a useful tool for improving cancer treatment. The present study aimed to investigate the role of miR-767 in non-small cell lung cancer (NSCLC) and further analyze the effects of UTMD-mediated miR-767 inhibition on tumor progression. The expression of miR-767 was measured by reverse transcription-quantitative PCR. UTMD-mediated miR-767 inhibition was achieved by the co-transfection of microbubbles and miR-767 inhibitor in NSCLC cells. Cell proliferation was assessed by a CCK-8 assay and cell migration and invasion were examined by a Transwell assay. The expression of miR-767 was increased in NSCLC serum, tissues and cells compared with controls. The reduction of miR-767 in NSCLC cells led to the inhibition of cell proliferation, migration and invasion. UTMD increased the transfection efficiency of the miR-767 inhibitor in NSCLC cells, and UTMD-mediated miR-767 inhibition resulted in a more significant suppressive effect on tumor cell proliferation, migration and invasion. Taken together, the results indicated that miR-767 expression is upregulated in both NSCLC clinical samples and cells. The downregulation of miR-767 can inhibit tumor cell proliferation, migration and invasion, and these effects are further promoted by UTMD-mediated miR-767 inhibition, which indicated the potential of a UTMD-mediated miR-767 inhibition as a novel therapeutic strategy for NSCLC treatment.

Ultrasound‑targeted microbubble destruction enhances the anti‑tumor action of miR‑4284 inhibitor in non‑small cell lung cancer cells

“…In the study of Yang et al (34), miR-let-7b was transfected into ovarian cancer stem cells by flow cytometry, and the results indicated that miR-let-7b transfection efficiency using UTMD was significantly higher. In NSCLC, the expression level of miR-767 could be successfully downregulated by miR-767 inhibitor and UTMD further enhanced the transfection efficacy of miR-767 inhibitor, while downregulation of miR-767 was indicated to inhibit the proliferation, migration and invasion of tumor cells (15). In the present study, UTMD improved the transfection efficiency of a miR-4284 inhibitor in NSCLC cells.…”

“…The present study aimed to evaluate the expression of miR-4284 in NSCLC tissues and cell lines and further explored the biological functions of miR-4284 in NSCLC progression. Several studies have reported on the enhancing effects of UTMD on the functional role of miRNAs in disease progression, UTMD-mediated miR-767 inhibition has been indicated to result in a notable suppressive effect of tumor cell proliferation, migration and invasion, and UTMD has also been revealed to assist the exosome delivery of miR-21, serving a protective role in the heart ( 14 , 15 ). Therefore, the present study further compared the differences in biological functional changes between UTMD-mediated and conventional transfection of miR-4284 in NSCLC cells.…”

Ultrasound-targeted microbubble destruction mediated miR-492 inhibitor suppresses the tumorigenesis in non-small cell lung cancer

“…considered that UTMD enhanced the synergistic treatment of rheumatoid arthritis via targeting liposomes [ 9 ]. Additively, research revealed that UTMD-mediated miR-767 inhibition resulted in a more significant suppressive effect on NSCLC tumour proliferation, migration and invasion [ 10 ]. Moreover, in other cancers or malignancies, such as hepatocellular carcinoma [ 11 ], synergistic therapeutic effects of UTMD were also widely recognized.…”

Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2

“…Interestingly, USMB-miR-133a prevented tumor growth and improved survival of breast cancer in mice. USMB-mediated miR-767 silencing offered a novel therapeutic strategy for non-small-cell lung cancer treatment [ 22 , 23 ]. However, the mechanism of USMB-miR-505 in CC remains unclear.…”