Ultrasound-targeted microbubble destruction mediated miR-492 inhibitor suppresses the tumorigenesis in non-small cell lung cancer

Abstract

Background Ultrasound-targeted microbubble destruction (UTMD) is a novel adjuvant tumor therapeutic method by enhancing exogenous gene transfection to target tissues. This study aims to investigate the role of microRNA-492 (miR-492) in non-small cell lung cancer (NSCLC) and further analyze the effects of UTMD-mediated miR-492 inhibitor on tumorigenesis. Methods The expression of miR-492 was detected by qRT-PCR. Co-transfection of microbubbles and miR-492 inhibitor with Lipofectamine 3000 was performed to achieve UTMD-mediated miR-492 inhibition in NSCLC cells. CCK-8 and Transwell assay were used to determine NSCLC cell proliferation, and the migration and invasion. Result High expression of miR-492 was associated with poor prognosis in NSCLC patients. miR-492 inhibitor suppressed tumor cell proliferation, migration and invasion, and UTMD not only increased the transfection efficiency of miR-492 inhibitor, but also enhance the inhibitory effects on cell biological behaviors. Conclusion The results showed that the expression level of miR-492 was up-regulated in NSCLC tissue samples and cells. Silencing of miR-492 inhibited NSCLC cell proliferation, migration and invasion, and UTMD-mediated miR-492 inhibitor could promote more significant inhibition, which indicated that UTMD-mediated miR-492 inhibitor might provide a novel strategy for the treatment of NSCLC. KEY MESSAGES miR-492 inhibitor inhibited cell proliferation, migration and invasion. UTMD-mediated miR-492 inhibitor can promote more significant inhibition. UTMD-mediated miR-492 inhibitor provide a new strategy for NSCLC.

The diagnostic value of has_circ_0006423 in non-small cell lung cancer and its role as a tumor suppressor gene that sponges miR-492

“…Zhu et al found a correlation between the upregulation of circRNA 100,876 and NSCLC lymph node metastasis 30 , hsa_circ_0013958 is thought to be a miR-134 sponge that gives rise to the upregulation of cancer-causing cyclin D1 in lung adenocarcinoma 31 . For the sponge miR-492 of hsa_circ_0006423 in this study, it was found that miR-492 was highly expressed in NSCLC serum 32 ; and silencing miR-492 inhibited the proliferation, migration and invasion of NSCLC cells 33 . This provides a basis for further research on the inhibition of NSCLC occurrence and development after the hsa_circ_0006423 sponge absorbs miR-492.…”

CircFBXW7 Inhibits Proliferation, Migration, and Invasion of Nonsmall Cell Lung Cancer Cells by Regulating miR-492

“…To explore the potential mechanism of action of circFBXW7 in NSCLC, we predicted the potential miRNAs of circFBXW7 by miRanda software and miTarBase database and plotted circRNA-miRNA interaction using Cytoscape software ( Figure 3(a) ). Among the miRNAs, miR-492 has been reported to regulate NSCLC progression [ 18 – 20 ]. Then, qRT-PCR detection showed that miR-492 expression was significantly downregulated in the oe-circFBXW7 group ( Figure 3(b) ).…”

“…erefore, we screened potential target miRNAs as predicted by miRanda software and miTarBase database combined with the results from previous studies. MiR-942 was selected for further study [18][19][20]. Zhang et al reported that overexpression of hsa_circ_0005567 promoted polarization of M2-type macrophages through the miR-492/SOCS2 axis and inhibited the progression of osteoarthritis [28].…”

“…Potential miRNAs of circFBXW7 were predicted through miRanda software and miTarBase database, and Cytoscape software was performed to plot the circRNA-miRNA regulatory network. In addition, miR-492 was reported to regulate NSCLC progression [18][19][20].…”

“…Besides, it has been reported that high expression of miR-492 was associated with poor prognosis of NSCLC patients. MiR-492 inhibitor inhibited the proliferation, migration, and invasion of tumor cells [ 18 ].…”