Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

Tavakolian, Shaian; Goudarzi, Hossein; Faghihloo, Ebrahim

doi:10.1186/s13104-020-05071-9

Cited by 12 publications

(8 citation statements)

References 39 publications

(39 reference statements)

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“…Previous study showed that hsa-miR-192 was a tumor suppressor. It could suppress cell proliferation, metastasis, and stemness and induce apoptosis in different cancer types [ 26 – 32 ]. But its function in CC remained unknown.…”

Section: Discussionmentioning

confidence: 99%

RacGAP1 promotes the malignant progression of cervical cancer by regulating AP-1 via miR-192 and p-JNK

Zhang

Wang

et al. 2022

Cell Death Dis

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Cervical cancer (CC) is the most frequently diagnosed genital tract cancer in females worldwide. Rac GTPase-activating protein 1 (RacGAP1) is one of the specific GTPase-activating proteins. As a novel tumor protooncogene, overexpression of RacGAP1 was related to the occurrence of various tumors, but its function in CC is still unclear. In this study, bioinformatics analyses showed that RacGAP1 might be a key candidate gene in the progression of CC. RacGAP1 was significantly overexpressed in CC tissues. High RacGAP1 expression was positively associated with poor prognosis. Downregulating RacGAP1 significantly inhibited the proliferation, migration, and invasion of CC cells, while overexpressing RacGAP1 had the opposite effects. Further research showed that miR-192, which plays as a tumor suppressor in CC, was identified as a downstream target of RacGAP1 in CC cells. miR-192 inhibition could partially rescue the decrease in cell proliferation, migration, and invasion caused by RacGAP1 downregulation. In opposite, miR-192 overexpression could decrease the promotion of malignant progression caused by RacGAP1 upregulation. Mechanism studies revealed that RacGAP1 could regulate the expression and phosphorylation of c-Jun, which was the component of AP-1, via miR-192 and p-JNK separately. These findings suggested that RacGAP1 promoted tumorigenicity, migration, and invasion of CC. Therefore, it represented a potential novel prognostic marker in CC and may probably be a therapeutic target.

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Section: Discussionmentioning

confidence: 99%

RacGAP1 promotes the malignant progression of cervical cancer by regulating AP-1 via miR-192 and p-JNK

Zhang

Wang

et al. 2022

Cell Death Dis

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“…Intriguingly, the metabolites produced by intestinal microflora can stimulate the expression of miR-192-5p, which subsequently inhibits the proliferation of colon cancer cells via the RhoA-ROCK-LIMK2 pathway ( Huang et al, 2020 ). A recent study by Tavakolian and coworkers demonstrated that the expression of miR-192-5p is significantly reduced in tissues of human gastric cancer, suggesting that miR-192-5p may play an important role in gastric cancer ( Tavakolian et al, 2020 ). Consistently, vital studies have indicated that miR-192-5p plays a role in the resistance of EC and gastric cancer to chemotherapy, which reverses cisplatin resistance by targeting ERCC3 and ERCC4 in gastric cancer lines ( Hummel et al, 2014 ; Xie et al, 2019 ).…”

Section: Mir-192-5p In Human Diseasesmentioning

confidence: 99%

Emerging Role of MiR-192-5p in Human Diseases

et al. 2021

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MicroRNAs (miRNAs) are a type of small non-coding RNAs that play an essential role in numerous biological processes by regulating the post-transcriptional expression of target genes. Recent studies have demonstrated that miR-192-5p, a member of the miR-192 family, partakes in several human diseases, especially various cancers, including cancers of the lung, liver, and breast. Importantly, the levels of miR-192-5p are abundant in biofluids, including the serum and urine, and the exosomal levels of miR-192-5p in circulation can aid in the diagnosis and prognosis of various diseases, such as chronic hepatitis B (CHB) infection disease. Notably, recent studies suggest that miR-192-5p is regulated by long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). However, there are no comprehensive overviews on the role of miR-192-5p in human diseases. This review discusses the significant studies on the role of miR-192-5p in various human diseases, with special emphasis on the diseases of the respiratory and digestive systems.

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“…In addition, the rescue by miR-9-5p inhibitor of the effect of ZNRD1-AS1 knockdown and the inhibition by the miR-9-5p binding-site mutant of the effect of ZNRD1-AS1 overexpression further demonstrated that ZNRD1-AS1 may act as a buffer for miR-9-5p. This hypothesis is further supported by the suppressive effect of miR-9-5p on the proliferation, migration, and invasion of gastric cancer cells [ 12 , 13 ]. However, the ZNRD1-AS1 sequence has many miRNA response elements, suggesting that ZNRD1-AS1 may play a role as a ceRNA of other miRNAs.…”

Section: Discussionmentioning

confidence: 92%

Knockdown of lncRNA ZNRD1-AS1 suppresses gastric cancer cell proliferation and metastasis by targeting the miR-9-5p/HSP90AA1 axis

Gong

et al. 2021

Aging

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LncRNAs play an important role in a variety of biological processes, such as cancer pathogenesis. The lncRNA zinc ribbon domain containing 1 antisense RNA 1 (ZNRD1-AS1) is a natural antisense transcript of ZNRD1. In this study, we found that ZNRD1-AS1 levels were significantly upregulated in gastric cancer tissues compared to those in adjacent healthy gastric tissues. ZNRD1-AS1 levels were correlated with lymph node metastasis, distal metastasis, and TNM stage, but were not correlated with age and sex. ZNRD1-AS1 knockdown suppressed cell proliferation, migration, and invasion, and promoted apoptosis. ZNRD1-AS1 overexpression had the opposite effect. ZNRD1-AS1 knockdown suppressed tumor growth and pulmonary metastasis in a nude mouse model ZNRD1-AS1 can bind to miR-9-5p and ZNRD1-AS1 knockdown can decrease the protein level of heat shock protein 90 alpha family class A member 1 (HSP90AA1), which is the target of miR-9-5p. The miR-9-5p inhibitor rescued the effect of ZNRD1-AS1 knockdown, and the mutant of miR-9-5p binding site on ZNRD1-AS1 sequence blocked the effect of ZNRD1-AS1 overexpression. In conclusion, ZNRD1-AS1 levels were upregulated in gastric cancer tissues, and knockdown of ZNRD1-AS1 suppressed gastric cancer cell proliferation and metastasis by targeting the miR-9-5p/HSP90AA1 axis. Our findings provide novel insights into the mechanism underlying the role of ZNRD1-AS1 in gastric cancer.

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Evaluating the expression level of miR-9-5p and miR-192-5p in gastrointestinal cancer: introducing novel screening biomarkers for patients

Cited by 12 publications

References 39 publications

RacGAP1 promotes the malignant progression of cervical cancer by regulating AP-1 via miR-192 and p-JNK

RacGAP1 promotes the malignant progression of cervical cancer by regulating AP-1 via miR-192 and p-JNK

Emerging Role of MiR-192-5p in Human Diseases

Knockdown of lncRNA ZNRD1-AS1 suppresses gastric cancer cell proliferation and metastasis by targeting the miR-9-5p/HSP90AA1 axis

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