Changes in metabolism, growth and nutrient uptake of Ulva fasciata (Chlorophyta) in response to nitrogen source

Shahar, Ben; Shpigel, Muki; Barkan, Roy; Masasa, Matan; Neori, Amir; Chernov, Helena; Salomon, Eitan; Kiflawi, Moshe; Guttman, Louis

doi:10.1016/j.algal.2019.101781

Cited by 20 publications

(20 citation statements)

References 71 publications

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“…However, nutrient concentrations, biomass densities and the period of the day contributed to statistical differences. Distinct results were obtained with U. fasciata cultivated with N-NO 3 − and total ammonium nitrogen (TAN) in which F v /F m was affected by the nitrogen source (Shahar et al 2020). In that study, U. fasciata was described to present higher F v /F m when cultivated with N-NO 3 − , and the authors justify the lower photosynthetic activity in cultures supplied with TAN than N-NO 3 − due to a possible toxicity of the free NH 3 + .…”

Section: Discussionmentioning

confidence: 98%

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

Massocato

Robles-Carnero

Vega

et al. 2022

Preprint

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Short-term N-NH4+ and N-NO3- removal by U. pseudorotundata under different initial nutrient concentrations and biomass stocking densities, monitoring in parallel photosynthetic parameters obtained by Pulse-Amplitude-Modulation (PAM) Fluorometry of chlorophyll-a and evaluating biomass characteristics (CHN, proteins, carbohydrates) was investigated. The results showed that U. pseudorotundata cultivated under solar radiation in a land-based out-door pilot scale was efficient in removing N-NH4+ and N-NO3- even in concentrations as high as 500 µmol L⁻¹ ensuring complete water remediation in a period of 24 h and in all experimental conditions tested, although differences were observed depending on the stocking density and type of N-source. Treatments with N-NH4+ showed faster N removal than those with N-NO3-, however, treatments with N-NO3- demanded more photosynthetic energy. Part of the ammonium removed in the respective treatments may have been volatilized as a combined effect of the pH increase related to photosynthesis and aeration in the tanks. The results presented here establish the fundamental parameters for the use of U. pseudorotundata as a biofiltering organism for effluents with high concentrations of N-NH4+ and N-NO3-.

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Section: Discussionmentioning

confidence: 98%

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

Massocato

Robles-Carnero

Vega

et al. 2022

Preprint

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“…Moreover, dried Ulva spp. biomass can contain up to 40% protein (Shpigel et al, 2019;Shahar et al, 2020), and is a source of cell wall polysaccharides that are sought after for biotechnology applications (Kidgell et al, 2019). In this context, Ulva spp.…”

Section: Introductionmentioning

confidence: 99%

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

et al. 2022

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Anthropogenic impacts on water resources, especially by nutrient discharge, is a worldwide problem in marine coastal areas. In this context, seaweed cultivation in aquaculture wastewater can be considered as an alternative for effluent mitigation, where the biomass becomes a source of valuable compounds. The current study examined the potential use of the seaweeds Ulva pseudorotundata and Ulva rigida to remove nutrients to treat effluents from the culture of Chelon labrosus. Two experiments were conducted under pilot-scale conditions to evaluate the nutrient uptake, photosynthetic activity, and biomass production of the seaweed species cultivated under 50 and 100% effluent concentrations. Photosynthetic parameters were determined by in vivo chlorophyll a fluorescence associated to photosystem II 3 times a day to estimate photosynthetic performance and seaweed physiology throughout the experiment: optimal quantum yield (Fv/Fm), in situ and ex situ electron transport rate (ETR), photosynthetic efficiency (αETR), saturation irradiance (Ek), and the maximum non-photochemical quenching (NPQmax). To evaluate seaweed metabolism and biomass compounds, elemental and biochemical composition were analyzed in the beginning and end of each experiment. Results regarding the nutrient source showed that both species removed more than 65% of ammonium after 3 hours of experimentation. At the end of the experiments, up to 94.8% of the initial ammonium was sequestered from the effluent. Additionally, after 5 days of cultivation under 50% fish effluent both Ulva species were able to remove more than 85% of the nitrate. Although a decrease in uptake efficiency was observed in cultures with 100% fish effluent, at the end of the experiment more than 440 µmol L-1 of nitrate was removed, considering all treatment conditions. The biomass values showed that growth rates of seaweed cultivated in 100% effluent were higher than those obtained in 50% effluent. Moreover, when cultivated in the 100% effluent concentration, a significant increment in protein content was detected in both Ulva species. Our results contribute to the understanding of biofiltration and photosynthetic performance of two different Ulva species in order to improve growth optimization, enhancement of biofiltration capacity and also to boost management practices of seaweed cultivation in aquaculture effluent treatment systems.

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“…Waite & Michell (1972) suggest that TAN is toxic to U. lactuca at concentrations >65 uM, whereas other studies (e.g. Fujita 1985, Ji et al 2019, Shahar et al 2020) applied higher concentrations and did not report reduced growth or degenerating seaweed. It seems however unlikely that the high nitrogen concentrations (ammonium nor nitrate) in our study were toxic, since no mortality or debilitation was observed, as seen for the Ulva cultured under the high orthophosphate concentration.…”

Section: Effect Of N-source (Nitrate or Ammonium) Under High Nitrogen...mentioning

confidence: 97%

“…In that respect they seem opposite to the results obtained during our acclimatization week, which could be regarded as Vs, and where lower growth was observed for the ammonium based treatments. Both Neori (1996) and Shahar et al (2020) found better growth of U. lactuca with ammonium than with nitrate for non-starved Ulva, representing Vm, and attributed this difference to the different uptake and assimilation pathways that are involved for the two N-sources. Interestingly, even though growth rates were comparable between nitrate and ammonium treatments in our study, higher tissue nitrogen contents were achieved in the ammonium based treatments, suggesting an accumulation of nitrogen which is not used for growth.…”

Section: Effect Of N-source (Nitrate or Ammonium) Under High Nitrogen...mentioning

confidence: 98%

“…The differential nutrient uptake and growth response of Ulva spp. to these nitrogen sources are related to differences in uptake and assimilation pathways, which affects the energy requirements (Shahar et al 2020): uptake and assimilation of nitrate uses much metabolic energy (active) while assimilation of TAN is passive and requires less energy (Taylor et al 2006). This suggests that seaweed growth in bacterial driven RAS IMTA systems might be sub-optimal since nitrogen is mostly available as nitrate-N.…”

Section: Introductionmentioning

confidence: 99%

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Integrated multi-trophic aquaculture : Nutrient retention efficiency and valorisation of waste nutrients

Nederlof¹

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14 ± 1 13 ± 1 12 ± 0.4 ns 20:1 (n-9) 2.1 ±0.09 a,b 2.2 ± 0.04 a 1.97 ± 0.06 b ** 22:1 (n-11) 2.89 ± 0.06 a 2.91 ±0.05 a 2.73 ±0.03 b *** 24:1 (n-9) 1.17 ± 0.01 c 1.29 ±0.02 a 1.20 ±0.02 b *** PUFA 9 ± 0.4 a 5 ± 0.2 c 6 ± 0.2 b *** PUFA (n-6) 4.7 ± 0.3 a 2.3 ± 0.1 c 3.6± 0.1 b *** 18:2 (n-6) 1 4.3 ±0.2 a 2.0 ±0.2 c 3.3 ±0.1 b *** 20:4 (n-6) 2 0.07 ± 0.004 a 0.03 ± 0.005 c 0.04 ± 0.003 b *** PUFA (n-3)3.6 ± 0.1 a 2.3 ±0.1 b 2.4 ± 0.1 b *** 18:3 (n-3) 3 0.8 ± 0.08 a 0.3 ± 0.02 c 0.4 ± 0.02 b *** 20:5 (n-3) 4 0.6 ± 0.03 a 0.4 ± 0.01 b 0.4 ± 0.02 b *** 22:6 (n-3) 5 0.8 ± 0.01 a 0.4 ± 0.02 b 0.4 ± 0.02 b *** NMI 0.4 ± 0.02 a 0.4 ± 0.01 a 0.4 ± 0.03 b ** PUFA/SFA 0.13 ± 0.009 a 0.07 ± 0.004 c 0.09 ±0.004 b ***

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Changes in metabolism, growth and nutrient uptake of Ulva fasciata (Chlorophyta) in response to nitrogen source

Cited by 20 publications

References 71 publications

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

Integrated multi-trophic aquaculture : Nutrient retention efficiency and valorisation of waste nutrients

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