Changes in Membrane Integrity, Cytoskeletal Structure, and Developmental Potential of Murine Oocytes after Vitrification in Ethylene Glycol1

Hotamisligil, Selen; Toner, Mehmet; Powers, Robert D.

doi:10.1095/biolreprod55.1.161

Cited by 104 publications

(45 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to their large molecular size, sucrose could cause an osmotic gradient across the cell membrane, which enhances dehydration of the cell before freezing of extracellular water. In addition, it is suggested that sucrose is capable of preserving the structural and functional integrity of cell membranes at low water activities [29]. Sucrose, as nonpermeating cryoprotectants, has been used in the vitrification of ovarian tissues.…”

Section: Discussionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…The addition of carbohydrates to permeable cryoprotectants aids the dehydration of embryos (Kuleshova et al, 1999). It has been suggested that sugars are capable of preserving the structural and functional integrity of membranes at low water activity (Crowe, Carpenter, Crowe, & Anchordoguy, 1990;Hotamisligil, Toner, & Powers, 1996). The design of proper protocol for cryoprotectant incorporation is decisive for the success of cryopreservation, but adequate freezing/thawing rates are also necessary (Cabrita et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Untitled

Keivanloo¹,

Sudagar²,

Abasali³

2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The effects of vitrification on the embryo viability of the Persian sturgeon (Acipenser persicus) were investigated in this study. Vitrificant solutions (VS1-VS6) were prepared by combining propylene glycol (PG) as basic cryoprotectant plus other permeable and non-permeable cryoprotectants in different concentration. Embryos at neurulation stage were selected and incubated in six vitrificant solutions for 7 minutes using four-step incorporation protocol before transferring into liquid nitrogen. Following these treatments, the embryos were washed well and incubated until hatching. The results showed that PG-based vitrificant solutions seemed to be suitable for the vitrification of Persian sturgeon embryos except VS4. After vitrification (-196°C for 10 min), the embryos were thawed (water bath, 0 or 20°C), rehydrated and incubated to determine survival rate. Results demonstrated that the embryos treated with solutions VS1, VS2, VS3, VS5 and VS6 presented some degrees of viability after vitrification, whereas none survived after vitrification in VS4. There were no differences in the results obtained using either 0 or 20°C thawing temperatures. Among six vitrificant solutions, the combination of 6 M PG + 5 M dimethyl sulfoxide +6 M ethylene glycol +3 M acetamide (VS1) had the highest survival rate (59.09%).

show abstract

“…Variações térmicas muito abruptas podem interferir no transporte de água através da membrana celular e propiciar a formação de cristais de gelo e depósitos de sais no interior da célula 11 . Também as diferenças de pressão osmótica entre o ambiente intra e o extracelular podem levar à mudança de volume no oócito, com conseqüente dano na membrana plasmática e nas organelas 12 . Além disso, também tem sido demonstrado que o congelamento pode danifi car o citoesqueleto dos folículos antrais, com prejuízo no tráfego de moléculas e organelas no processo de divisão celular 13 .…”

Section: Criopreservaçãounclassified

Preservação de fertilidade

Silva

Sá²

2006

Rev. Bras. Ginecol. Obstet.

View full text Add to dashboard Cite

RESUMOCom a evolução dos recursos terapêuticos e o aumento das taxas de sobrevida dos pacientes oncológicos, as repercussões tardias destas terapias, que antes eram infreqüentes, hoje assumem um papel importante quando se fala em qualidade de vida. Dentre estas complicações tardias está a perda da função ovariana. Segundo as recomendações da Sociedade Americana de Oncologia Clínica (American Society of Clinical Oncology) recentemente publicadas, os métodos comprovadamente efi cazes para preservação da fertilidade feminina disponíveis hoje são: o congelamento de embriões, a cirurgia ginecológica conservadora e a ooforopexia para os casos de radioterapia localizada. Todas as demais técnicas existentes, tais como a supressão ovariana medicamentosa e a criopreservação de tecido ovariano e de oócitos, embora apresentem resultados promissores, ainda são consideradas experimentais. A escolha da melhor técnica para preservação de fertilidade aplicável em cada caso vai depender da idade da paciente, do tipo de tratamento, da existência ou não de parceiro com quem deseje constituir prole, do tempo disponível até o início da quimioterapia e do potencial do câncer em produzir metástase ovariana. Neste artigo as técnicas disponíveis e experimentais para a preservação da fertilidade são revisadas e discutidas. PALAVRAS-CHAVE:Falência ovariana prematura; Criopreservação; Quimioterapia; Radioterapia; Infertilidade ABSTRACTAs therapeutic approaches for oncologic diseases are being improved and an increase in the survival rates are being achieved, long-term complications of these therapies, initially infrequent, assume these days an important place when considering life quality. Among the long term repercussions appears the premature ovarian failure. According to the recommendations of the American Society of Clinical Oncology recently published, the only procedures available nowadays considered to be effective for female fertility preservation are: embryo cryopreservation, conservative gynecological surgery and oophoropexy in cases of local radiotherapy. All the other proposed techniques, surch as: ovarian suppression and oocyte and ovarian tissue cryopreservation, although present promising results, are still considered as experimental options. The best choice for fertility preservation in each specifi c case depends on patient's age, type of treatment, existence of a partner, time available until chemo-or radiotherapy beginning, and the ovarian metastatic potential of the tumor. In the present manuscript, the available and experimental techniques for fertility preservation are revised and discussed. KEYWORDS:

show abstract

Changes in Membrane Integrity, Cytoskeletal Structure, and Developmental Potential of Murine Oocytes after Vitrification in Ethylene Glycol1

Cited by 104 publications

References 29 publications

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Untitled

Preservação de fertilidade

Contact Info

Product

Resources

About