Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatids

Catena, Raffaella; Ronfani, Lorenza; Sassone–Corsi, Paolo; Davidson, Irwin

doi:10.1016/j.ydbio.2006.04.458

Cited by 32 publications

(36 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…H1T2 has a peculiar localization pattern, as it preferentially associates with chromatin located at the apical pole of both round and elongating spermatids (Martianov et al 2005). This localization depends on proteins involved in chromatin organization such as TRF2 and HMGB2 (Catena et al 2006). In Intron (Mandl et al 1997) Long UTRs and polyA tail SpH1…”

Section: Structure Conservation and Expression Patternmentioning

confidence: 99%

Germline-specific H1 variants: the “sexy” linker histones

2015

View full text Add to dashboard Cite

Section: Structure Conservation and Expression Patternmentioning

confidence: 99%

Germline-specific H1 variants: the “sexy” linker histones

2015

View full text Add to dashboard Cite

“…This is accompanied by the appearance of testis-specific linker histones (Catena et al, 2006;Martianov et al, 2005;Tanaka et al, 2005;Yan et al, 2003). So far, no linker histone variants have been identified in Drosophila, but variants of H2A (H2AvD) and H3 (H3.3) are known (Akhmanova et al, 1997;Swaminathan et al, 2005).…”

Section: Histone Degradation Correlates With High Level Of Ubiquitin mentioning

confidence: 99%

“…First, testisspecific linker histones appear (Catena et al, 2006;Martianov et al, 2005;Tanaka et al, 2005;Yan et al, 2003), and then histones are replaced by transition proteins (major types: TP1 and TP2), which in turn are replaced by protamines, leading to condensed chromatin with a doughnut structure (reviewed by Braun, 2001;Kimmins and Sassone-Corsi, 2005;SassoneCorsi, 2002). Recently, we have shown that Drosophila sperm also contain protamines as well as Mst77F, representing at least one further chromatin component, whereas transition proteinlike chromatin components have not been found in Drosophila so far (Jayaramaiah-Raja and Renkawitz-Pohl, 2005).…”

Section: Introductionmentioning

confidence: 99%

Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis inDrosophila

Rathke

Baarends

Jayaramaiah-Raja

et al. 2007

Journal of Cell Science

203

264

View full text Add to dashboard Cite

presence of the architectural protein CTCF, numerous DNA breaks, SUMO, UbcD6 and high content of ubiquitin, as well as testes-specific nuclear proteasomes at this time. Moreover, we report the first transition protein-like chromosomal protein, Tpl 94D, to be found in Drosophila. We propose that Tpl 94D -an HMG box protein -and the numerous DNA breaks facilitate chromatin unwinding as a prelude to protamine and Mst77F deposition. Finally, we show that histone modifications and removal are independent of protamine synthesis. ), the presence of CTCF and DNA breaks. We furthermore show that histone removal is independent of the presence of protamines. Both this histone removal and protamine accumulation are essential for transmission of the male genome to the oocyte, and therefore of fundamental importance for the persistence of species. ResultsCore histones and their variants are removed simultaneously from the DNA of spermatid nuclei prior to protamine accumulation In Drosophila melanogaster, sperm morphogenesis, i.e. from meiosis until sperm individualisation, lasts 3.5 days. After meiosis, the nucleus initially is round and then gradually changes its shape accompanied by reorganisation of the chromatin during the canoe stage (Jayaramaiah-Raja and Renkawitz-Pohl, 2005), resulting in sperm containing a slim nucleus in which the nuclear volume is decreased by a factor of 200 (for review see Fuller, 1993;Renkawitz-Pohl et al., 2005).In the work reported here we concentrated on post-meiotic sperm morphogenesis with particular focus on chromatin reorganisation from the nucleosomal-to the protamine-based structure, which is a dramatic switch. Previously, we have reported that the histone variant H2AvD-GFP vanishes at the canoe stage while protamines begin to accumulate simultaneously (Jayaramaiah-Raja and Renkawitz-Pohl, 2005). To analyse the timing of histone removal and protamine accumulation we brought protamine-eGFP and H2AvD-RFP (Clarkson and Saint, 1999) into one genetic background to enable a study in the same individual. We found that H2AvD-RFP disappeared before protamine-eGFP accumulation took place (data not shown). We then went on to immunostain testes of protamine-eGFP flies with an antibody recognising all histones. This antibody was raised against total histones of humans and detects all core histones and the linker histone H1 in mammals. As -in contrast to core histones -H1 is not well conserved between mammals and Drosophila, we presumably detect solely core histones with this antibody. Our findings show that core histones (Fig. 1A) are detectable up to the canoe stage whereas protamines start to be synthesised at the canoe stage (Fig. 1B) but with no apparent positional overlap. As the canoe stage is quite long, we defined the early canoe stage by the start of histone removal, and the late canoe stage by the start of protamine accumulation (see Fig. 1A-E, second and third columns). With histone H3.3, a further replacement variant is expressed in the testis and disappears in post-meiotic stages togethe...

show abstract

“…In another study, it was noted that H1t2 2/2 mice show greatly reduced fertility and delayed nuclear condensation, aberrant elongation, acrosomal detachment, and fragmented DNA (Martianov et al, 2005). It has been documented that proper acrosome development is not needed for the specific H1T2 localization, but that H1T2 is altered to a bipolar localization in the nucleus when proteins involved in intranuclear chromatin architecture are modified (Catena et al, 2006 (Govin et al, 2007).…”

Section: Microscopy Research and Techniquementioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 2: Changes in spermatid organelles associated with development of spermatozoa

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

View full text Add to dashboard Cite

Spermiogenesis is a long process whereby haploid spermatids derived from the meiotic divisions of spermatocytes undergo metamorphosis into spermatozoa. It is subdivided into distinct steps with 19 being identified in rats, 16 in mouse and 8 in humans. Spermiogenesis extends over 22.7 days in rats and 21.6 days in humans. In this part, we review several key events that take place during the development of spermatids from a structural and functional point of view. During early spermiogenesis, the Golgi apparatus forms the acrosome, a lysosome-like membrane bound organelle involved in fertilization. The endoplasmic reticulum undergoes several topographical and structural modifications including the formation of the radial body and annulate lamellae. The chromatoid body is fully developed and undergoes structural and functional modifications at this time. It is suspected to be involved in RNA storing and processing. The shape of the spermatid head undergoes extensive structural changes that are species-specific, and the nuclear chromatin becomes compacted to accommodate the stream-lined appearance of the sperm head. Microtubules become organized to form a curtain or manchette that associates with spermatids at specific steps of their development. It is involved in maintenance of the sperm head shape and trafficking of proteins in the spermatid cytoplasm. During spermiogenesis, many genes/proteins have been implicated in the diverse dynamic events occurring at this time of development of germ cells and the absence of some of these have been shown to result in subfertility or infertility.

show abstract

Changes in intranuclear chromatin architecture induce bipolar nuclear localization of histone variant H1T2 in male haploid spermatids

Cited by 32 publications

References 26 publications

Germline-specific H1 variants: the “sexy” linker histones

Germline-specific H1 variants: the “sexy” linker histones

Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis inDrosophila

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 2: Changes in spermatid organelles associated with development of spermatozoa

Contact Info

Product

Resources

About