Changes in inositol transport during DMSO-induced differentiation of HL60 cells towards neutrophils

Baxter, Michael A.; Bunce, Christopher M.; Lord, Janet M.; French, Philip J.; Michell, Robert H.; Brown, Geoffrey

doi:10.1016/0167-4889(91)90056-4

Cited by 13 publications

(14 citation statements)

References 24 publications

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“…The assay of inositol transport used in this study has been validated in reports that established that inositol transport in the human promyeloid cell line HL60 is active, specific, saturable, and Na + dependent (13,15). These studies have established that HL60 is a suitable model system for studies of the mechanisms that regulate inositol transport.…”

Section: Resultsmentioning

confidence: 85%

“…These studies have established that HL60 is a suitable model system for studies of the mechanisms that regulate inositol transport. Furthermore, the similarity in the K m for inositol transport in many cell and tissue systems indicates that a common transport protein, with potentially identical regulatory mechanisms, is expressed in all tissues that actively transport inositol (13,15).…”

Section: Resultsmentioning

confidence: 99%

“…These properties are characteristics of a carrier-mediated transport system. The similarity in the K m values for the inositol transporter in different cells suggests that a common carrier is expressed in all cells that actively transport inositol (13). The human promyeloid cell line HL60 expresses an inositol transporter that has a K m value identical to that in the human peripheral blood neutrophil (13,18).…”

Section: Discussionmentioning

confidence: 99%

“…Cell culture and the assay of inositol transport were performed as described previously (13). HL60 cells were maintained in long-term exponential growth in RPMI-1640 medium (Gibco, Paisley, Scotland, UK) supplemented with 10% fetal calf serum, L-glutamine (Gibco), and antibiotics (100 U/ml penicillin and 50 jig/ml streptomycin, Gibco).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we used the promyeloid cell line HL60, which has been validated as a model system (13). In addition, a recent study has shown that myeloid cells undergo metabolic changes that are characteristic of tissues involved in the development of diabetic complications (14).…”

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confidence: 99%

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Effect of Mg2+ on Na+-Dependent Inositol Transport: Role for Mg2+ in Etiology of Diabetic Complications

et al. 1992

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Diabetes mellitus is associated with a significant reduction in the serum concentration of Mg2+. Several studies have suggested that hypomagnesemia may be implicated in the etiology of diabetic complications; however, no mechanism has been proposed. This study demonstrates that Mg2+ is a positive effector of inositol transport and is capable of promoting a 2.5-fold increase in the affinity of the transporter for inositol. Analysis of the kinetics of inositol transport shows that, at physiological concentrations of inositol, the reductions in Mg2+ concentrations that occur in diabetic patients would result in a significant decline in the rate of inositol transport (1.5- to 2-fold). We suggest that hypomagnesemia may be linked to the development of diabetic complications via reduction in the rate of inositol transport and subsequent intracellular inositol depletion. This assertion allows hypomagnesemia and the polyol theory to be unified into one mechanistic model for the development of diabetic complications.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Mg2+ on Na+-Dependent Inositol Transport: Role for Mg2+ in Etiology of Diabetic Complications

et al. 1992

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Inositol Lipids and Phosphates in the Proliferation and Differentiation of Lymphocytes and Myeloid Cells

Michell

Finney

et al. 2007

Ciba Foundation Symposium 164 ‐ Interactions Among Cell Signalling Systems

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Sodium-independent carrier-mediated inositol transport in cultured renal epithelial (LLC-PK1) cells

Russo

Marano

Hagee

et al. 1995

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In addition to the concentrative, Na(+)-dependent inositol transport system demonstrated in many cell types, carrier-mediated, Na(+)-independent inositol transport is also shown to exist in LLC-PK1 renal epithelia. Inhibition of inositol uptake in Na(+)-free saline by 0.1 mM phloretin, and self-inhibition by net concentrations of inositol exceeding 10 mM, demonstrate the carrier-mediation of the Na(+)-independent uptake and distinguish it from flux through anion channels. The Na(+)-dependent uptake exhibits higher affinity for inositol, as seen by the stronger self-inhibition at lower inositol concentrations in Na+ saline. Kinetic analyses indicate a Km of 178 microM and a Vmax of 2447 pmol/min per microgram DNA for the Na(+)-dependent system, whereas the lower affinity, lower capacity Na(+)-independent system manifests a Km of 5.2 mM and a Vmax of 249 pmol/min per microgram DNA. the Na(+)-independent uptake further differs from the Na(+)-dependent transport by the lack of inhibitory effect of 10 microM glucose, and the greater relative inhibition of phloretin compared to that of phlorizin. Both types of uptake appear to localize predominantly to the basal-lateral cell surface. The Na(+)-independent transport is bidirectional, functioning in efflux as well as influx of inositol.

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Changes in inositol transport during DMSO-induced differentiation of HL60 cells towards neutrophils

Cited by 13 publications

References 24 publications

Effect of Mg2+ on Na+-Dependent Inositol Transport: Role for Mg2+ in Etiology of Diabetic Complications

Effect of Mg2+ on Na+-Dependent Inositol Transport: Role for Mg2+ in Etiology of Diabetic Complications

Inositol Lipids and Phosphates in the Proliferation and Differentiation of Lymphocytes and Myeloid Cells

Sodium-independent carrier-mediated inositol transport in cultured renal epithelial (LLC-PK1) cells

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