Inositol Lipids and Phosphates in the Proliferation and Differentiation of Lymphocytes and Myeloid Cells

Michell, Robert H.; La, Conroy; Finney, Michael; Pj, French; Cm, Bunce; Anderson, Kim L.; Ma, Baxter; Brown, Geoffrey; Gordon, John; Ej, Jenkinson

doi:10.1002/9780470514207.ch2

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…SphK1 and S1P have been implicated in mast cell calcium mobilization from intracellular stores (Choi et al, 1996;Melendez and Khaw, 2002). Considerable evidence argues for a dominant role of inositol (3,4,5)-trisphosphate (IP 3 ), rather than S1P, in mobilizing calcium in mast cells, as well as other immune cells (Michell et al, 1992). Conversely, other studies suggested that the Fc3RI principally utilized SphK to mobilize calcium (Choi et al, 1996;Melendez and Khaw, 2002).…”

Section: Sphk2-deficient Mast Cells Have Defective Calcium Influxmentioning

confidence: 93%

The Sphingosine Kinase-Sphingosine-1-Phosphate Axis Is a Determinant of Mast Cell Function and Anaphylaxis

et al. 2007

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Sphingosine-1-phosphate, a key mediator in immune cell trafficking, is elevated in the lungs of asthmatic patients and regulates pulmonary epithelium permeability. Stimulation of mast cells by allergens induces two mammalian sphingosine kinases (Sphk1 and Sphk2) to produce sphingosine-1-phosphate (S1P). Little is known about the individual role of these kinases in regulating immune cell function. Here we show that in mast cells, Sphk2 is required for production of S1P, for calcium influx, for activation of protein kinase C, and for cytokine production and degranulation. However, susceptibility to in vivo anaphylaxis is determined both by S1P within the mast cell compartment and by circulating S1P generated by Sphk1 predominantly from a non-mast cell source(s). Thus, sphingosine kinases are determinants of mast cell responsiveness, demonstrating a previously unrecognized relationship with anaphylaxis.

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Section: Sphk2-deficient Mast Cells Have Defective Calcium Influxmentioning

confidence: 93%

The Sphingosine Kinase-Sphingosine-1-Phosphate Axis Is a Determinant of Mast Cell Function and Anaphylaxis

et al. 2007

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“…Calcium release from intracellular stores by IgE/Ag involves PLCγ activation and inositol (3,4,5)-trisphosphate (IP 3 ) formation [49], although others have also implicated SphK1 and S1P in mast cell calcium mobilization [34,36]. However, release from intracellular stores cannot account for the overall calcium responses in mast cells, and instead, the extracellular calcium pool appears to be the major contributor for calcium and mast cell responses [31].…”

Section: 22-sphk2-induced Calcium Regulation-mentioning

confidence: 99%

Unraveling the complexities of sphingosine-1-phosphate function: The mast cell model

Olivera

2008

Prostaglandins & Other Lipid Mediators

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Sphingosine-1-phosphate (S1P) is a lipid mediator involved in diverse biological processes, from vascular and neural development to the regulation of lymphocyte trafficking. Many of its functions are regulated by five widely expressed S1P G-protein-coupled receptors (S1P(1-5)). S1P is produced mostly intracellularly, thus, much of its potential as an autocrine and paracrine mediator depends on how, when, and where it is generated or secreted out of the cells. However, S1P can also have intracellular activity independent of its receptors, adding to the complexity of S1P function. The mast cell, a major effector cell during an allergic response, has proven instrumental towards understanding the complex regulation and function of S1P. Antigen (Ag) engagement of the IgE receptor in mast cells stimulates sphingosine kinases, which generate S1P and are involved in the activation of calcium fluxes critical for mast cell responses. In addition, mast cells secrete considerable amounts of S1P upon activation, thus affecting the surrounding tissues and recruiting inflammatory cells. Export of S1P is also involved in the autocrine transactivation of S1P receptors present in mast cells. The in vivo response of mast cells, however, is not strictly dependent on their ability to generate S1P, but they are also affected by changes in S1P in the environment previous to Ag challenge. This review will discuss the recent advances towards understanding the intricacies of S1P generation, secretion and regulation in mast cells. In addition, how S1P receptors are activated and their involvement in mast cell functions will also be covered, including new insights on the role of S1P in the mast cell-mediated allergic response of systemic anaphylaxis.

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“…Recentemente, evidências têm sugerido funções na regulação da maturação e reciclagem endossomal, regulação do citoesqueleto de actina, tráfego entre membranas, migração e proliferação celular, autofagia, apoptose e expressão gênica (23,(26)(27)(28)(29)(30)(31)(32). Nas células hematopoiéticas, há evidências de que este fosfoinositídeo participe da resposta a estímulos extracelulares, como por exemplo a Interleucina 4 (IL-4) os quais resultam na ativação de genes específicos em linfócitos B, na ativação de linfócitos T, diferenciação de células HL-60 em neutrófilos e monócitos, indução da ativação plaquetária e na produção de espécies reativas de oxigênio em neutrófilos (33)(34)(35)(36).…”

Section: As Talassemias unclassified

Avaliação da síntese de globinas após a superexpressão do gene da fosfatidilinositol-4-fosfato-5-quinase-II-alfa ("PIP4K2A")

Takaki¹

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A ata de defesa com as respectivas assinaturas dos membros da banca examinadora encontra-se no processo de vida acadêmica do aluno. Data: DATA DA DEFESA 30/01/2020Não deixaremos de explorar e, ao término da nossa exploração, deveremos chegar ao ponto de partida e conhecer esse lugar pela primeira vez. T. S. Eliot DEDICATÓRIA Dedico este trabalho aos meus pais Carlos e Valéria, os maiores incentivadores dos meus sonhos. Esta pesquisa é a prova de que todo o investimento e dedicação que me proporcionaram geram frutos magníficos. Meu eterno amor e gratidão. AGRADECIMENTOS Meus maiores agradecimentos são direcionados à Dra. Susan Jorge, minha orientadora, e à Profa. Fátima Sonati. Pela oportunidade de trabalhar no Laboratório de Hemoglobinopatias e cujo conhecimento e amizade serviram de pilares de sustentação desse projeto, meu eterno agradecimento! Agradeço ao meu companheiro e amor, Rafael, cuja paciência, compreensão e conselhos fizeram-se presentes ao longo desta caminhada. Agradeço ao meu parceiro de projeto, Eliel, por toda a parceria, incentivo, troca de ideias e amizade. Agradeço a minha amiga e parceira, Paula Lima, cuja ajuda foi fundamental para o término deste trabalho. Agradeço às belíssimas amizades dos meus amigos Marcela, Bruna, Paula L., Gisele, Paula N, Francine, Vincent e Will. Por cada conversa, risadas, conselhos e ajuda que me deram durante esta jornada. Ao Prof. Magnun, pela amizade, conhecimento transmitido e oportunidade de aprendizado junto à docência.Aos colegas Amauri e Érika, cujos conselhos, ideias e ensinamentos também se fazem presentes neste trabalho.Às "meninas" do Hemocentro/Unicamp: Carol, Dul e Irene.Ao Prof. Julio e à Universidad de la República (UDELAR), por me receberem de braços abertos e pela incrível experiência e aprendizado.Este trabalho com realizado com apoio financeiro da Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), processo nº 2014/00984-3

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Inositol Lipids and Phosphates in the Proliferation and Differentiation of Lymphocytes and Myeloid Cells

Cited by 4 publications

References 29 publications

The Sphingosine Kinase-Sphingosine-1-Phosphate Axis Is a Determinant of Mast Cell Function and Anaphylaxis

The Sphingosine Kinase-Sphingosine-1-Phosphate Axis Is a Determinant of Mast Cell Function and Anaphylaxis

Unraveling the complexities of sphingosine-1-phosphate function: The mast cell model

Avaliação da síntese de globinas após a superexpressão do gene da fosfatidilinositol-4-fosfato-5-quinase-II-alfa ("PIP4K2A")

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